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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-144512268-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=144512268&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 144512268,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000617875.6",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3112C>T",
          "hgvs_p": "p.Arg1038Cys",
          "transcript": "NM_004260.4",
          "protein_id": "NP_004251.4",
          "transcript_support_level": null,
          "aa_start": 1038,
          "aa_end": null,
          "aa_length": 1208,
          "cds_start": 3112,
          "cds_end": null,
          "cds_length": 3627,
          "cdna_start": 3161,
          "cdna_end": null,
          "cdna_length": 3819,
          "mane_select": "ENST00000617875.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3112C>T",
          "hgvs_p": "p.Arg1038Cys",
          "transcript": "ENST00000617875.6",
          "protein_id": "ENSP00000482313.2",
          "transcript_support_level": 1,
          "aa_start": 1038,
          "aa_end": null,
          "aa_length": 1208,
          "cds_start": 3112,
          "cds_end": null,
          "cds_length": 3627,
          "cdna_start": 3161,
          "cdna_end": null,
          "cdna_length": 3819,
          "mane_select": "NM_004260.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2041C>T",
          "hgvs_p": "p.Arg681Cys",
          "transcript": "ENST00000621189.4",
          "protein_id": "ENSP00000483145.1",
          "transcript_support_level": 1,
          "aa_start": 681,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": 2041,
          "cds_end": null,
          "cds_length": 2556,
          "cdna_start": 3238,
          "cdna_end": null,
          "cdna_length": 3896,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3187C>T",
          "hgvs_p": "p.Arg1063Cys",
          "transcript": "NM_001413019.1",
          "protein_id": "NP_001399948.1",
          "transcript_support_level": null,
          "aa_start": 1063,
          "aa_end": null,
          "aa_length": 1233,
          "cds_start": 3187,
          "cds_end": null,
          "cds_length": 3702,
          "cdna_start": 3236,
          "cdna_end": null,
          "cdna_length": 3894,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3112C>T",
          "hgvs_p": "p.Arg1038Cys",
          "transcript": "NM_001413036.1",
          "protein_id": "NP_001399965.1",
          "transcript_support_level": null,
          "aa_start": 1038,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 3112,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": 3161,
          "cdna_end": null,
          "cdna_length": 3828,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3082C>T",
          "hgvs_p": "p.Arg1028Cys",
          "transcript": "NM_001413039.1",
          "protein_id": "NP_001399968.1",
          "transcript_support_level": null,
          "aa_start": 1028,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 3082,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": 3131,
          "cdna_end": null,
          "cdna_length": 3789,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3016C>T",
          "hgvs_p": "p.Arg1006Cys",
          "transcript": "NM_001413020.1",
          "protein_id": "NP_001399949.1",
          "transcript_support_level": null,
          "aa_start": 1006,
          "aa_end": null,
          "aa_length": 1176,
          "cds_start": 3016,
          "cds_end": null,
          "cds_length": 3531,
          "cdna_start": 3065,
          "cdna_end": null,
          "cdna_length": 3723,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2983C>T",
          "hgvs_p": "p.Arg995Cys",
          "transcript": "NM_001413025.1",
          "protein_id": "NP_001399954.1",
          "transcript_support_level": null,
          "aa_start": 995,
          "aa_end": null,
          "aa_length": 1165,
          "cds_start": 2983,
          "cds_end": null,
          "cds_length": 3498,
          "cdna_start": 3032,
          "cdna_end": null,
          "cdna_length": 3690,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2980C>T",
          "hgvs_p": "p.Arg994Cys",
          "transcript": "NM_001413033.1",
          "protein_id": "NP_001399962.1",
          "transcript_support_level": null,
          "aa_start": 994,
          "aa_end": null,
          "aa_length": 1164,
          "cds_start": 2980,
          "cds_end": null,
          "cds_length": 3495,
          "cdna_start": 3029,
          "cdna_end": null,
          "cdna_length": 3687,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2914C>T",
          "hgvs_p": "p.Arg972Cys",
          "transcript": "NM_001413018.1",
          "protein_id": "NP_001399947.1",
          "transcript_support_level": null,
          "aa_start": 972,
          "aa_end": null,
          "aa_length": 1142,
          "cds_start": 2914,
          "cds_end": null,
          "cds_length": 3429,
          "cdna_start": 2963,
          "cdna_end": null,
          "cdna_length": 3621,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2818C>T",
          "hgvs_p": "p.Arg940Cys",
          "transcript": "NM_001413017.1",
          "protein_id": "NP_001399946.1",
          "transcript_support_level": null,
          "aa_start": 940,
          "aa_end": null,
          "aa_length": 1110,
          "cds_start": 2818,
          "cds_end": null,
          "cds_length": 3333,
          "cdna_start": 2867,
          "cdna_end": null,
          "cdna_length": 3525,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2761C>T",
          "hgvs_p": "p.Arg921Cys",
          "transcript": "NM_001413029.1",
          "protein_id": "NP_001399958.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 1091,
          "cds_start": 2761,
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          "cds_length": 3276,
          "cdna_start": 2810,
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          "cdna_length": 3468,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2116C>T",
          "hgvs_p": "p.Arg706Cys",
          "transcript": "NM_001413023.1",
          "protein_id": "NP_001399952.1",
          "transcript_support_level": null,
          "aa_start": 706,
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          "aa_length": 876,
          "cds_start": 2116,
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          "cds_length": 2631,
          "cdna_start": 3301,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2050C>T",
          "hgvs_p": "p.Arg684Cys",
          "transcript": "NM_001413041.1",
          "protein_id": "NP_001399970.1",
          "transcript_support_level": null,
          "aa_start": 684,
          "aa_end": null,
          "aa_length": 854,
          "cds_start": 2050,
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          "cdna_start": 3297,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          ],
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2041C>T",
          "hgvs_p": "p.Arg681Cys",
          "transcript": "NM_001413021.1",
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 17,
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          "exon_count": 20,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2041C>T",
          "hgvs_p": "p.Arg681Cys",
          "transcript": "NM_001413022.1",
          "protein_id": "NP_001399951.1",
          "transcript_support_level": null,
          "aa_start": 681,
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          "cds_start": 2041,
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2041C>T",
          "hgvs_p": "p.Arg681Cys",
          "transcript": "NM_001413024.1",
          "protein_id": "NP_001399953.1",
          "transcript_support_level": null,
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          "cdna_start": 3123,
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        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2041C>T",
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          "transcript": "NM_001413028.1",
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        },
        {
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          "strand": false,
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          ],
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          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
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          "hgvs_c": "c.2041C>T",
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          "transcript": "NM_001413034.1",
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        },
        {
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2041C>T",
          "hgvs_p": "p.Arg681Cys",
          "transcript": "NM_001413035.1",
          "protein_id": "NP_001399964.1",
          "transcript_support_level": null,
          "aa_start": 681,
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          "cds_length": 2556,
          "cdna_start": 3058,
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          "cdna_length": 3716,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "RECQL4",
      "gene_hgnc_id": 9949,
      "dbsnp": "rs763078132",
      "frequency_reference_population": 0.000047755257,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 77,
      "gnomad_exomes_af": 0.0000465704,
      "gnomad_genomes_af": 0.0000591195,
      "gnomad_exomes_ac": 68,
      "gnomad_genomes_ac": 9,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.09484028816223145,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.10000000149011612,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": 0.0977,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.24,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.634,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.1,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000617875.6",
          "gene_symbol": "RECQL4",
          "hgnc_id": 9949,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.3112C>T",
          "hgvs_p": "p.Arg1038Cys"
        }
      ],
      "clinvar_disease": "Baller-Gerold syndrome,Rapadilino syndrome,Rothmund-Thomson syndrome type 2",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:3",
      "phenotype_combined": "Baller-Gerold syndrome|Rapadilino syndrome;Baller-Gerold syndrome;Rothmund-Thomson syndrome type 2",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}