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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-71317617-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=71317617&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 71317617,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001370333.1",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "NM_000503.6",
          "protein_id": "NP_000494.2",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
          "cds_end": null,
          "cds_length": 1779,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000340726.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000503.6"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "ENST00000340726.8",
          "protein_id": "ENSP00000342626.3",
          "transcript_support_level": 1,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
          "cds_end": null,
          "cds_length": 1779,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000503.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000340726.8"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "ENST00000388742.8",
          "protein_id": "ENSP00000373394.4",
          "transcript_support_level": 1,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
          "cds_end": null,
          "cds_length": 1779,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000388742.8"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.476T>C",
          "hgvs_p": "p.Leu159Pro",
          "transcript": "ENST00000419131.6",
          "protein_id": "ENSP00000410176.1",
          "transcript_support_level": 1,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 557,
          "cds_start": 476,
          "cds_end": null,
          "cds_length": 1674,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000419131.6"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.578T>C",
          "hgvs_p": "p.Leu193Pro",
          "transcript": "NM_001370333.1",
          "protein_id": "NP_001357262.1",
          "transcript_support_level": null,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 578,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001370333.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.578T>C",
          "hgvs_p": "p.Leu193Pro",
          "transcript": "ENST00000643681.1",
          "protein_id": "ENSP00000495390.1",
          "transcript_support_level": null,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": 578,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000643681.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "NM_001370334.1",
          "protein_id": "NP_001357263.1",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
          "cds_end": null,
          "cds_length": 1779,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001370334.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "NM_001370335.1",
          "protein_id": "NP_001357264.1",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
          "cds_end": null,
          "cds_length": 1779,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001370335.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "NM_172058.4",
          "protein_id": "NP_742055.1",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
          "cds_end": null,
          "cds_length": 1779,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_172058.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "ENST00000645793.1",
          "protein_id": "ENSP00000496255.1",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
          "cds_end": null,
          "cds_length": 1779,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000645793.1"
        },
        {
          "aa_ref": "L",
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "ENST00000647540.1",
          "protein_id": "ENSP00000494438.1",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
          "cds_end": null,
          "cds_length": 1779,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000647540.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
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          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "ENST00000862515.1",
          "protein_id": "ENSP00000532574.1",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 592,
          "cds_start": 491,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "L",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.491T>C",
          "hgvs_p": "p.Leu164Pro",
          "transcript": "ENST00000862516.1",
          "protein_id": "ENSP00000532575.1",
          "transcript_support_level": null,
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          "cds_start": 491,
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          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "L",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.488T>C",
          "hgvs_p": "p.Leu163Pro",
          "transcript": "ENST00000388743.6",
          "protein_id": "ENSP00000373395.2",
          "transcript_support_level": 5,
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          "aa_end": null,
          "aa_length": 591,
          "cds_start": 488,
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          "cdna_start": null,
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        },
        {
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          "consequences": [
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          "intron_rank": null,
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          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.488T>C",
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          "transcript": "ENST00000862517.1",
          "protein_id": "ENSP00000532576.1",
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          "cds_start": 488,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000862517.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.488T>C",
          "hgvs_p": "p.Leu163Pro",
          "transcript": "ENST00000964980.1",
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          "transcript_support_level": null,
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          "cds_start": 488,
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        },
        {
          "aa_ref": "L",
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          "consequences": [
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          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.476T>C",
          "hgvs_p": "p.Leu159Pro",
          "transcript": "ENST00000862518.1",
          "protein_id": "ENSP00000532577.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 587,
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        {
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        {
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          ],
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          "gene_symbol": "EYA1",
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          "biotype": "protein_coding",
          "feature": "NM_001288574.2"
        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EYA1",
          "gene_hgnc_id": 3519,
          "hgvs_c": "c.563T>C",
          "hgvs_p": "p.Leu188Pro",
          "transcript": "NM_172059.5",
          "protein_id": "NP_742056.2",
          "transcript_support_level": null,
          "aa_start": 188,
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          "cds_start": 563,
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          "biotype": "protein_coding",
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        {
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      ],
      "gene_symbol": "EYA1",
      "gene_hgnc_id": 3519,
      "dbsnp": "rs1007684729",
      "frequency_reference_population": 0.000011771528,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 19,
      "gnomad_exomes_af": 0.0000123132,
      "gnomad_genomes_af": 0.00000656927,
      "gnomad_exomes_ac": 18,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7273845672607422,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.675,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.4512,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.49,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.325,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS2",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001370333.1",
          "gene_symbol": "EYA1",
          "hgnc_id": 3519,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.578T>C",
          "hgvs_p": "p.Leu193Pro"
        }
      ],
      "clinvar_disease": "Branchiootic syndrome 1,Branchiootorenal syndrome 1,Melnick-Fraser syndrome,Otofaciocervical syndrome 1,not provided,not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:4",
      "phenotype_combined": "not specified|not provided|Melnick-Fraser syndrome|Branchiootic syndrome 1;Branchiootorenal syndrome 1;Otofaciocervical syndrome 1",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}