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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 9-131085653-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=9&pos=131085653&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "9",
      "pos": 131085653,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_006059.4",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.4160C>T",
          "hgvs_p": "p.Ala1387Val",
          "transcript": "NM_006059.4",
          "protein_id": "NP_006050.3",
          "transcript_support_level": null,
          "aa_start": 1387,
          "aa_end": null,
          "aa_length": 1575,
          "cds_start": 4160,
          "cds_end": null,
          "cds_length": 4728,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000361069.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006059.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.4160C>T",
          "hgvs_p": "p.Ala1387Val",
          "transcript": "ENST00000361069.9",
          "protein_id": "ENSP00000354360.4",
          "transcript_support_level": 2,
          "aa_start": 1387,
          "aa_end": null,
          "aa_length": 1575,
          "cds_start": 4160,
          "cds_end": null,
          "cds_length": 4728,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_006059.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361069.9"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.203C>T",
          "hgvs_p": "p.Ala68Val",
          "transcript": "ENST00000355452.5",
          "protein_id": "ENSP00000347627.5",
          "transcript_support_level": 1,
          "aa_start": 68,
          "aa_end": null,
          "aa_length": 256,
          "cds_start": 203,
          "cds_end": null,
          "cds_length": 771,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000355452.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.4178C>T",
          "hgvs_p": "p.Ala1393Val",
          "transcript": "ENST00000868026.1",
          "protein_id": "ENSP00000538085.1",
          "transcript_support_level": null,
          "aa_start": 1393,
          "aa_end": null,
          "aa_length": 1581,
          "cds_start": 4178,
          "cds_end": null,
          "cds_length": 4746,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868026.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.4154C>T",
          "hgvs_p": "p.Ala1385Val",
          "transcript": "ENST00000955224.1",
          "protein_id": "ENSP00000625283.1",
          "transcript_support_level": null,
          "aa_start": 1385,
          "aa_end": null,
          "aa_length": 1573,
          "cds_start": 4154,
          "cds_end": null,
          "cds_length": 4722,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955224.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.4160C>T",
          "hgvs_p": "p.Ala1387Val",
          "transcript": "ENST00000955225.1",
          "protein_id": "ENSP00000625284.1",
          "transcript_support_level": null,
          "aa_start": 1387,
          "aa_end": null,
          "aa_length": 1567,
          "cds_start": 4160,
          "cds_end": null,
          "cds_length": 4704,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955225.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.4061C>T",
          "hgvs_p": "p.Ala1354Val",
          "transcript": "ENST00000955223.1",
          "protein_id": "ENSP00000625282.1",
          "transcript_support_level": null,
          "aa_start": 1354,
          "aa_end": null,
          "aa_length": 1542,
          "cds_start": 4061,
          "cds_end": null,
          "cds_length": 4629,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955223.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.3851C>T",
          "hgvs_p": "p.Ala1284Val",
          "transcript": "ENST00000940484.1",
          "protein_id": "ENSP00000610543.1",
          "transcript_support_level": null,
          "aa_start": 1284,
          "aa_end": null,
          "aa_length": 1472,
          "cds_start": 3851,
          "cds_end": null,
          "cds_length": 4419,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940484.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.320C>T",
          "hgvs_p": "p.Ala107Val",
          "transcript": "ENST00000678758.1",
          "protein_id": "ENSP00000503612.1",
          "transcript_support_level": null,
          "aa_start": 107,
          "aa_end": null,
          "aa_length": 287,
          "cds_start": 320,
          "cds_end": null,
          "cds_length": 864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000678758.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "c.4178C>T",
          "hgvs_p": "p.Ala1393Val",
          "transcript": "XM_011518121.2",
          "protein_id": "XP_011516423.1",
          "transcript_support_level": null,
          "aa_start": 1393,
          "aa_end": null,
          "aa_length": 1581,
          "cds_start": 4178,
          "cds_end": null,
          "cds_length": 4746,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011518121.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "n.1733C>T",
          "hgvs_p": null,
          "transcript": "ENST00000678544.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000678544.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMC3",
          "gene_hgnc_id": 6494,
          "hgvs_c": "n.-172C>T",
          "hgvs_p": null,
          "transcript": "ENST00000462567.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000462567.1"
        }
      ],
      "gene_symbol": "LAMC3",
      "gene_hgnc_id": 6494,
      "dbsnp": "rs141497885",
      "frequency_reference_population": 0.00071863114,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 1160,
      "gnomad_exomes_af": 0.000585563,
      "gnomad_genomes_af": 0.00199556,
      "gnomad_exomes_ac": 856,
      "gnomad_genomes_ac": 304,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.007573336362838745,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.229,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1074,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.3,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.973,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BP6",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_006059.4",
          "gene_symbol": "LAMC3",
          "hgnc_id": 6494,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.4160C>T",
          "hgvs_p": "p.Ala1387Val"
        }
      ],
      "clinvar_disease": "LAMC3-related disorder,Occipital pachygyria and polymicrogyria,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:3 LB:2",
      "phenotype_combined": "not specified|not provided|Occipital pachygyria and polymicrogyria|LAMC3-related disorder",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}