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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: M-14706-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=M&pos=14706&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "M",
      "pos": 14706,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000000000",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": null,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRNE",
          "gene_hgnc_id": 7479,
          "hgvs_c": "c.37T>C",
          "hgvs_p": "p.Ser13Pro",
          "transcript": "unassigned_transcript_4817",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": 13,
          "aa_end": null,
          "aa_length": 22,
          "cds_start": 37,
          "cds_end": null,
          "cds_length": 69,
          "cdna_start": 37,
          "cdna_end": null,
          "cdna_length": 69,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MT-TE",
          "gene_hgnc_id": 7479,
          "hgvs_c": "n.37T>C",
          "hgvs_p": null,
          "transcript": "ENST00000387459.1",
          "protein_id": null,
          "transcript_support_level": 6,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 69,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MT-CYB",
          "gene_hgnc_id": 7427,
          "hgvs_c": "c.-41A>G",
          "hgvs_p": null,
          "transcript": "ENST00000361789.2",
          "protein_id": "ENSP00000354554.2",
          "transcript_support_level": 6,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 379,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1141,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1141,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MT-ND6",
          "gene_hgnc_id": 7462,
          "hgvs_c": "c.-33T>C",
          "hgvs_p": null,
          "transcript": "ENST00000361681.2",
          "protein_id": "ENSP00000354665.2",
          "transcript_support_level": 6,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 174,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 525,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 525,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": null,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYTB",
          "gene_hgnc_id": 7427,
          "hgvs_c": "c.-41A>G",
          "hgvs_p": null,
          "transcript": "unassigned_transcript_4818",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 379,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1141,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1141,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": null,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ND6",
          "gene_hgnc_id": 7462,
          "hgvs_c": "c.-33T>C",
          "hgvs_p": null,
          "transcript": "unassigned_transcript_4816",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 174,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 525,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 525,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "TRNE",
      "gene_hgnc_id": 7479,
      "dbsnp": "rs1057516070",
      "frequency_reference_population": 0.0001,
      "hom_count_reference_population": 8,
      "allele_count_reference_population": 8,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": 7,
      "gnomad_mito_heteroplasmic": 2,
      "computational_score_selected": 1.8867499828338623,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "Mitotip",
      "splice_score_selected": null,
      "splice_prediction_selected": null,
      "splice_source_selected": null,
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": -0.312,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": null,
      "spliceai_max_prediction": null,
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": 1.8867499828338623,
      "mitotip_prediction": "Benign",
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP6_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000000000",
          "gene_symbol": "TRNE",
          "hgnc_id": 7479,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "Mitochondrial",
          "hgvs_c": "c.37T>C",
          "hgvs_p": "p.Ser13Pro"
        },
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000387459.1",
          "gene_symbol": "MT-TE",
          "hgnc_id": 7479,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "Mitochondrial",
          "hgvs_c": "n.37T>C",
          "hgvs_p": null
        },
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000361789.2",
          "gene_symbol": "MT-CYB",
          "hgnc_id": 7427,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "AR,Mitochondrial",
          "hgvs_c": "c.-41A>G",
          "hgvs_p": null
        },
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000361681.2",
          "gene_symbol": "MT-ND6",
          "hgnc_id": 7462,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "Mitochondrial",
          "hgvs_c": "c.-33T>C",
          "hgvs_p": null
        },
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000000000",
          "gene_symbol": "CYTB",
          "hgnc_id": 7427,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "AR,Mitochondrial",
          "hgvs_c": "c.-41A>G",
          "hgvs_p": null
        },
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000000000",
          "gene_symbol": "ND6",
          "hgnc_id": 7462,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "Mitochondrial",
          "hgvs_c": "c.-33T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Developmental delay,Episodic vomiting,MELAS syndrome,Mild liver congestion,Sudden death,not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "B:1 O:1",
      "phenotype_combined": "Mild liver congestion;Episodic vomiting;Sudden death|Developmental delay|not provided|MELAS syndrome",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}