DNA Complement Generator
Generate the complementary DNA strand for any nucleotide sequence (A↔T, C↔G).
Complementary Sequence:
The result will appear here.
This tool generates the complementary DNA sequence by replacing each nucleotide with its Watson-Crick complement: A is replaced with T, T with A, G with C, and C with G.
Complementary sequences play a fundamental role in DNA replication, hybridization, and PCR primer design.
Paste your DNA sequence below to instantly compute the complementary strand.
FAQ
What is a DNA complement?
A DNA complement is the sequence formed by replacing each nucleotide with its base-pairing partner: A↔T and C↔G. This represents the antiparallel strand in a double-stranded DNA molecule.
What is the difference between complement and reverse complement?
The complement keeps the same 5′→3′ order but replaces each base. The reverse complement additionally reverses the sequence, representing the actual sequence of the opposing strand read 5′→3′.
When is the complement used?
DNA complements are used in probe design, primer design, and understanding base pairing in hybridization experiments.
Can this tool handle long sequences?
Yes, the tool supports long DNA sequences used in research and bioinformatics.