NM_004898.4:c.-44+2656G>A

Variant names:

• chr4-55486718-C-T
• rs11133389
• NM_004898.4:c.-44+2656G>A

Variant summary

Our verdict is Benign. The variant received -12 ACMG points: 0P and 12B. BP4_Strong BA1

The NM_004898.4(CLOCK):​c.-44+2656G>A variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.304 in 152,022 control chromosomes in the GnomAD database, including 7,639 homozygotes. In-silico tool predicts a benign outcome for this variant. No clinical diagnostic laboratories have submitted clinical-significance assessments for this variant to ClinVar.

• Frequency: Genomes: 𝑓 0.30 (7639 hom., cov: 32)
• Consequence: CLOCK NM_004898.4 intron
• Clinical Significance: Not reported in ClinVar
• Conservation: PhyloP100: -0.0270
• Publications: 7 publications found

Genes affected

CLOCK (HGNC:2082): (clock circadian regulator) The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

ACMG classification

Our verdict: Benign. The variant received -12 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.83).
• BA1: GnomAd4 highest subpopulation (EAS) allele frequency at 95% confidence interval = 0.565 is higher than 0.05.

Variant Effect in Transcripts

ACMG analysis was done for transcript: NM_004898.4. You can select a different transcript below to see updated ACMG assignments.

RefSeq Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	CLOCK	NM_004898.4	MANE Select	c.-44+2656G>A		intron	N/A	NP_004889.1
	CLOCK	NM_001267843.2		c.-200-169G>A		intron	N/A	NP_001254772.1

Ensembl Transcripts

Selected	Gene	Transcript	Tags	HGVSc	HGVSp	Effect	Exon Rank	Protein	UniProt
	CLOCK	ENST00000513440.6	TSL:1 MANE Select	c.-44+2656G>A		intron	N/A	ENSP00000426983.1
	CLOCK	ENST00000309964.8	TSL:1	c.-44+2656G>A		intron	N/A	ENSP00000308741.4
	CLOCK	ENST00000381322.5	TSL:1	c.-200-169G>A		intron	N/A	ENSP00000370723.1

Frequencies

GnomAD3 genomes AF: 0.304 AC: 46235 AN: 151904 Hom.: 7630 Cov.: 32

Gnomad AFR AF: 0.189

Gnomad AMI AF: 0.271

Gnomad AMR AF: 0.412

Gnomad ASJ AF: 0.405

Gnomad EAS AF: 0.582

Gnomad SAS AF: 0.413

Gnomad FIN AF: 0.357

Gnomad MID AF: 0.306

Gnomad NFE AF: 0.309

Gnomad OTH AF: 0.312

We have no GnomAD4 exomes data on this position. Probably position not covered by the project.

GnomAD4 genome AF: 0.304 AC: 46243 AN: 152022 Hom.: 7639 Cov.: 32 AF XY: 0.313 AC XY: 23228 AN XY: 74324

African (AFR) AF: 0.188 AC: 7810 AN: 41486

American (AMR) AF: 0.412 AC: 6296 AN: 15274

Ashkenazi Jewish (ASJ) AF: 0.405 AC: 1404 AN: 3468

East Asian (EAS) AF: 0.582 AC: 3006 AN: 5164

South Asian (SAS) AF: 0.412 AC: 1983 AN: 4818

European-Finnish (FIN) AF: 0.357 AC: 3772 AN: 10570

Middle Eastern (MID) AF: 0.301 AC: 88 AN: 292

European-Non Finnish (NFE) AF: 0.309 AC: 20970 AN: 67938

Other (OTH) AF: 0.317 AC: 668 AN: 2104

Alfa AF: 0.300 Hom.: 909

Bravo AF: 0.306

Asia WGS AF: 0.466 AC: 1621 AN: 3476

ClinVar

Not reported in ClinVar

Computational scores

Source: dbNSFP v4.9

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.83
CADD	Benign	2.9
DANN	Benign	0.50
PhyloP100		-0.027
Mutation Taster		=100/0	polymorphism (auto)

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs11133389; hg19: chr4-56352885;