NM_007078.3:c.860-15C>T

Variant names:

• chr10-86692520-C-T
• rs727503126
• NM_007078.3:c.860-15C>T

Variant summary

Our verdict is Likely benign. The variant received -5 ACMG points: 0P and 5B. BP4_Strong BP6

The NM_007078.3(LDB3):​c.860-15C>T variant causes a intron change involving the alteration of a non-conserved nucleotide. The variant allele was found at a frequency of 0.00000547 in 1,461,756 control chromosomes in the GnomAD database, with no homozygous occurrence. In-silico tool predicts a benign outcome for this variant. Variant has been reported in ClinVar as Conflicting classifications of pathogenicity (no stars).

• Frequency:
  • Genomes: not found (cov: 33)
  • Exomes 𝑓: 0.0000055 (0 hom.)
• Consequence: LDB3 NM_007078.3 intron
• Clinical Significance: Conflicting classifications of pathogenicity criteria provided, conflicting classifications U:1 B:3
• Conservation: PhyloP100: -0.0850
• Publications: 0 publications found

Genes affected

LDB3 (HGNC:15710): (LIM domain binding 3) This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains. [provided by RefSeq, Jan 2010]

LDB3 Gene-Disease associations (from GenCC):

• myofibrillar myopathy 4

  Inheritance: AD Classification: STRONG, MODERATE, LIMITED Submitted by: G2P, Labcorp Genetics (formerly Invitae), Ambry Genetics
• familial isolated dilated cardiomyopathy

  Inheritance: AD Classification: SUPPORTIVE Submitted by: Orphanet
• dilated cardiomyopathy

  Inheritance: AD Classification: LIMITED Submitted by: ClinGen
• familial dilated cardiomyopathy

  Inheritance: AR Classification: LIMITED Submitted by: Ambry Genetics
• arrhythmogenic right ventricular cardiomyopathy

  Inheritance: AD Classification: NO_KNOWN Submitted by: ClinGen

ENSG00000289258 (HGNC:):

ACMG classification

Our verdict: Likely_benign. The variant received -5 ACMG points.

• BP4: Computational evidence support a benign effect (BayesDel_noAF=-0.69).
• BP6: Variant 10-86692520-C-T is Benign according to our data. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841. Variant chr10-86692520-C-T is described in CliVar as Conflicting_classifications_of_pathogenicity. Clinvar id is 163841.

Transcripts

RefSeq

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	MANE	Protein	UniProt
LDB3	NM_007078.3	c.860-15C>T		intron_variant	Intron 6 of 13	ENST00000361373.9	NP_009009.1
LDB3	NM_001368067.1	c.719-15C>T		intron_variant	Intron 7 of 8	ENST00000263066.11	NP_001354996.1

Ensembl

Gene	Transcript	HGVSc	HGVSp	Effect	Exon rank	TSL	MANE	Protein	Appris	UniProt
LDB3	ENST00000361373.9	c.860-15C>T		intron_variant	Intron 6 of 13	1	NM_007078.3	ENSP00000355296.3
LDB3	ENST00000263066.11	c.719-15C>T		intron_variant	Intron 7 of 8	1	NM_001368067.1	ENSP00000263066.7
ENSG00000289258	ENST00000443292.2	c.2369-15C>T		intron_variant	Intron 16 of 17	1		ENSP00000393132.2

Frequencies

GnomAD3 genomes Cov.: 33

GnomAD2 exomes AF: 0.00000398 AC: 1 AN: 251488 AF XY: 0.00

Gnomad AFR exome AF: 0.00

Gnomad AMR exome AF: 0.00

Gnomad ASJ exome AF: 0.00

Gnomad EAS exome AF: 0.00

Gnomad FIN exome AF: 0.00

Gnomad NFE exome AF: 0.00000879

Gnomad OTH exome AF: 0.00

GnomAD4 exome AF: 0.00000547 AC: 8 AN: 1461756 Hom.: 0 Cov.: 31 AF XY: 0.00000550 AC XY: 4 AN XY: 727192

African (AFR) AF: 0.00 AC: 0 AN: 33478

American (AMR) AF: 0.00 AC: 0 AN: 44722

Ashkenazi Jewish (ASJ) AF: 0.00 AC: 0 AN: 26136

East Asian (EAS) AF: 0.00 AC: 0 AN: 39698

South Asian (SAS) AF: 0.0000116 AC: 1 AN: 86256

European-Finnish (FIN) AF: 0.00 AC: 0 AN: 53412

Middle Eastern (MID) AF: 0.00 AC: 0 AN: 5768

European-Non Finnish (NFE) AF: 0.00000630 AC: 7 AN: 1111894

Other (OTH) AF: 0.00 AC: 0 AN: 60392

GnomAD4 genome Cov.: 33

Alfa AF: 0.00 Hom.: 0

ClinVar

Significance: Conflicting classifications of pathogenicity

Submissions summary: Uncertain:1 Benign:3

Revision: criteria provided, conflicting classifications

Submissions by phenotype

not specified Uncertain:1 Benign:2

Aug 17, 2017

GeneDx

Significance: Likely benign

Review Status: criteria provided, single submitter

Collection Method: clinical testing

This variant is considered likely benign or benign based on one or more of the following criteria: it is a conservative change, it occurs at a poorly conserved position in the protein, it is predicted to be benign by multiple in silico algorithms, and/or has population frequency not consistent with disease. -

Jan 02, 2014

Laboratory for Molecular Medicine, Mass General Brigham Personalized Medicine

Significance: Uncertain significance

Review Status: criteria provided, single submitter

Collection Method: clinical testing

The 860-15C>T variant in LDB3 has not been previously reported in individuals wi th cardiomyopathy or in large population studies (European American and African American cohorts). This variant is located in the 3' splice region. Computationa l tools do not predict an impact to splicing, though this information is not pre dictive enough to rule out pathogenicity. Additional information is needed to fu lly assess the clinical significance of this variant. -

May 05, 2025

Women's Health and Genetics/Laboratory Corporation of America, LabCorp

Significance: Likely benign

Review Status: criteria provided, single submitter

Collection Method: clinical testing

- -

Myofibrillar myopathy 4 Benign:1

May 22, 2023

Labcorp Genetics (formerly Invitae), Labcorp

Significance: Likely benign

Review Status: criteria provided, single submitter

Collection Method: clinical testing

- -

Computational scores

Source: dbNSFP v4.3

Name	Calibrated prediction	Score	Prediction
BayesDel_noAF	Benign	-0.69
CADD	Benign	4.7
DANN	Benign	0.67
PhyloP100		-0.085

Splicing

Name	Calibrated prediction	Score	Prediction
SpliceAI score (max)		0.0		Details are displayed if max score is > 0.2

Find out detailed SpliceAI scores and Pangolin per-transcript scores at spliceailookup.broadinstitute.org

Other links and lift over

dbSNP: rs727503126; hg19: chr10-88452277;