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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-150077169-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=150077169&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 150077169,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000644510.2",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "NM_007259.5",
          "protein_id": "NP_009190.2",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 570,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 1713,
          "cdna_start": 596,
          "cdna_end": null,
          "cdna_length": 2328,
          "mane_select": "ENST00000644510.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000644510.2",
          "protein_id": "ENSP00000495563.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 570,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 1713,
          "cdna_start": 596,
          "cdna_end": null,
          "cdna_length": 2328,
          "mane_select": "NM_007259.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698584.1",
          "protein_id": "ENSP00000513813.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": 636,
          "cdna_end": null,
          "cdna_length": 2449,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000644526.2",
          "protein_id": "ENSP00000494363.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 643,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 1932,
          "cdna_start": 636,
          "cdna_end": null,
          "cdna_length": 2462,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698580.1",
          "protein_id": "ENSP00000513809.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 595,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 1788,
          "cdna_start": 636,
          "cdna_end": null,
          "cdna_length": 2140,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698582.1",
          "protein_id": "ENSP00000513811.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 582,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 1749,
          "cdna_start": 636,
          "cdna_end": null,
          "cdna_length": 4196,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.550T>C",
          "hgvs_p": "p.Cys184Arg",
          "transcript": "ENST00000698587.1",
          "protein_id": "ENSP00000513816.1",
          "transcript_support_level": null,
          "aa_start": 184,
          "aa_end": null,
          "aa_length": 582,
          "cds_start": 550,
          "cds_end": null,
          "cds_length": 1749,
          "cdna_start": 672,
          "cdna_end": null,
          "cdna_length": 2398,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698598.1",
          "protein_id": "ENSP00000513825.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 582,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 1749,
          "cdna_start": 568,
          "cdna_end": null,
          "cdna_length": 2336,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.511T>C",
          "hgvs_p": "p.Cys171Arg",
          "transcript": "ENST00000698590.1",
          "protein_id": "ENSP00000513818.1",
          "transcript_support_level": null,
          "aa_start": 171,
          "aa_end": null,
          "aa_length": 569,
          "cds_start": 511,
          "cds_end": null,
          "cds_length": 1710,
          "cdna_start": 607,
          "cdna_end": null,
          "cdna_length": 2322,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698581.1",
          "protein_id": "ENSP00000513810.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 548,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 1647,
          "cdna_start": 636,
          "cdna_end": null,
          "cdna_length": 2022,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.445T>C",
          "hgvs_p": "p.Cys149Arg",
          "transcript": "ENST00000698532.1",
          "protein_id": "ENSP00000513778.1",
          "transcript_support_level": null,
          "aa_start": 149,
          "aa_end": null,
          "aa_length": 547,
          "cds_start": 445,
          "cds_end": null,
          "cds_length": 1644,
          "cdna_start": 527,
          "cdna_end": null,
          "cdna_length": 2242,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.442T>C",
          "hgvs_p": "p.Cys148Arg",
          "transcript": "ENST00000419023.4",
          "protein_id": "ENSP00000400143.3",
          "transcript_support_level": 3,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 546,
          "cds_start": 442,
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          "cdna_start": 453,
          "cdna_end": null,
          "cdna_length": 2168,
          "mane_select": null,
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          "biotype": null,
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        },
        {
          "aa_ref": "C",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
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          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698578.1",
          "protein_id": "ENSP00000513807.1",
          "transcript_support_level": null,
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          "cds_start": 514,
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          "cds_length": 1629,
          "cdna_start": 636,
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          "mane_select": null,
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          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
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          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.406T>C",
          "hgvs_p": "p.Cys136Arg",
          "transcript": "NM_001279354.2",
          "protein_id": "NP_001266283.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 534,
          "cds_start": 406,
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          "cdna_start": 616,
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          "cdna_length": 2348,
          "mane_select": null,
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          "biotype": null,
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        },
        {
          "aa_ref": "C",
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          "protein_coding": true,
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          "consequences": [
            "missense_variant"
          ],
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          "gene_symbol": "VPS45",
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          "hgvs_p": "p.Cys136Arg",
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        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698528.1",
          "protein_id": "ENSP00000513777.1",
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          "cds_start": 514,
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          "cdna_length": 2281,
          "mane_select": null,
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        },
        {
          "aa_ref": "C",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
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          "intron_rank": null,
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          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698588.1",
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          "cdna_start": 621,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "C",
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          "strand": true,
          "consequences": [
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          ],
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          "gene_symbol": "VPS45",
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          "hgvs_c": "c.379T>C",
          "hgvs_p": "p.Cys127Arg",
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        {
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          "protein_coding": true,
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          "gene_symbol": "VPS45",
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          "hgvs_c": "c.514T>C",
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        },
        {
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          "strand": true,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg",
          "transcript": "ENST00000698534.1",
          "protein_id": "ENSP00000513780.1",
          "transcript_support_level": null,
          "aa_start": 172,
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          "cds_start": 514,
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          "cds_length": 1545,
          "cdna_start": 596,
          "cdna_end": null,
          "cdna_length": 2160,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "VPS45",
          "gene_hgnc_id": 14579,
          "hgvs_c": "c.514T>C",
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        },
        {
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      ],
      "gene_symbol": "VPS45",
      "gene_hgnc_id": 14579,
      "dbsnp": "rs191692092",
      "frequency_reference_population": 0.00004150884,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 67,
      "gnomad_exomes_af": 0.0000253107,
      "gnomad_genomes_af": 0.000197003,
      "gnomad_exomes_ac": 37,
      "gnomad_genomes_ac": 30,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.06075632572174072,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.377,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.7571,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.22,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.795,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000644510.2",
          "gene_symbol": "VPS45",
          "hgnc_id": 14579,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.514T>C",
          "hgvs_p": "p.Cys172Arg"
        }
      ],
      "clinvar_disease": "Congenital neutropenia-myelofibrosis-nephromegaly syndrome,Inborn genetic diseases,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:3",
      "phenotype_combined": "Congenital neutropenia-myelofibrosis-nephromegaly syndrome|Inborn genetic diseases|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}