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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-156732325-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=156732325&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 156732325,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_015997.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "NM_015997.4",
          "protein_id": "NP_057081.3",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 475,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 1428,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000368216.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015997.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "ENST00000368216.9",
          "protein_id": "ENSP00000357199.4",
          "transcript_support_level": 1,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 475,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 1428,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_015997.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000368216.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.-26C>T",
          "hgvs_p": null,
          "transcript": "ENST00000476229.5",
          "protein_id": "ENSP00000430787.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 154,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 465,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000476229.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.-26C>T",
          "hgvs_p": null,
          "transcript": "ENST00000484742.5",
          "protein_id": "ENSP00000429053.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 99,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 302,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000484742.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.-26C>T",
          "hgvs_p": null,
          "transcript": "XM_047421937.1",
          "protein_id": "XP_047277893.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047421937.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "ENST00000917461.1",
          "protein_id": "ENSP00000587520.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 459,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 1380,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917461.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "ENST00000892486.1",
          "protein_id": "ENSP00000562545.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892486.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "ENST00000519086.5",
          "protein_id": "ENSP00000429756.1",
          "transcript_support_level": 3,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 344,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 1035,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000519086.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "ENST00000892485.1",
          "protein_id": "ENSP00000562544.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 309,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 930,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892485.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "ENST00000892483.1",
          "protein_id": "ENSP00000562542.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 290,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 873,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892483.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.275C>T",
          "hgvs_p": "p.Thr92Met",
          "transcript": "ENST00000917458.1",
          "protein_id": "ENSP00000587517.1",
          "transcript_support_level": null,
          "aa_start": 92,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 275,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917458.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
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          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "NM_001142560.2",
          "protein_id": "NP_001136032.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 277,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 834,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001142560.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "ENST00000368218.8",
          "protein_id": "ENSP00000357201.4",
          "transcript_support_level": 3,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 277,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 834,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000368218.8"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "XM_005245234.6",
          "protein_id": "XP_005245291.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 443,
          "cds_start": 281,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
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          "consequences": [
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          ],
          "exon_rank": 3,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.83C>T",
          "hgvs_p": "p.Thr28Met",
          "transcript": "XM_011509600.2",
          "protein_id": "XP_011507902.1",
          "transcript_support_level": null,
          "aa_start": 28,
          "aa_end": null,
          "aa_length": 409,
          "cds_start": 83,
          "cds_end": null,
          "cds_length": 1230,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011509600.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.83C>T",
          "hgvs_p": "p.Thr28Met",
          "transcript": "XM_017001404.2",
          "protein_id": "XP_016856893.1",
          "transcript_support_level": null,
          "aa_start": 28,
          "aa_end": null,
          "aa_length": 409,
          "cds_start": 83,
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          "cds_length": 1230,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_017001404.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "XM_047421932.1",
          "protein_id": "XP_047277888.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
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          "cds_start": 281,
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          "cds_length": 1122,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "XM_047421932.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
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          "protein_coding": true,
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          ],
          "exon_rank": 3,
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "XM_005245236.6",
          "protein_id": "XP_005245293.1",
          "transcript_support_level": null,
          "aa_start": 94,
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          "aa_length": 328,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 987,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005245236.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
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          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "XM_005245237.6",
          "protein_id": "XP_005245294.1",
          "transcript_support_level": null,
          "aa_start": 94,
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          "cds_start": 281,
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          "cds_length": 966,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005245237.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "METTL25B",
          "gene_hgnc_id": 24273,
          "hgvs_c": "c.281C>T",
          "hgvs_p": "p.Thr94Met",
          "transcript": "XM_024447421.2",
          "protein_id": "XP_024303189.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 281,
          "cds_end": null,
          "cds_length": 891,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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      "dbsnp": "rs1163529192",
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      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.29,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.054,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.06,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
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          "score": 2,
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          "verdict": "Uncertain_significance",
          "transcript": "NM_015997.4",
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          "effects": [
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          "inheritance_mode": "",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
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  ],
  "message": null
}