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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-16440811-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=16440811&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 16440811,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001145277.2",
      "consequences": [
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "NM_018090.5",
          "protein_id": "NP_060560.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 263,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 792,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000337132.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_018090.5"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000337132.10",
          "protein_id": "ENSP00000338746.5",
          "transcript_support_level": 1,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 263,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 792,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_018090.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000337132.10"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "NM_001145277.2",
          "protein_id": "NP_001138749.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145277.2"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000443980.6",
          "protein_id": "ENSP00000391942.2",
          "transcript_support_level": 2,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000443980.6"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000966887.1",
          "protein_id": "ENSP00000636946.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966887.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000966888.1",
          "protein_id": "ENSP00000636947.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966888.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000893002.1",
          "protein_id": "ENSP00000563061.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000893002.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000916912.1",
          "protein_id": "ENSP00000586971.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 205,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 618,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916912.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000504551.6",
          "protein_id": "ENSP00000424509.1",
          "transcript_support_level": 5,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 202,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 609,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000504551.6"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000893001.1",
          "protein_id": "ENSP00000563060.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 167,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 504,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000893001.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "ENST00000916911.1",
          "protein_id": "ENSP00000586970.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 105,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 318,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916911.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg",
          "transcript": "XM_047424715.1",
          "protein_id": "XP_047280671.1",
          "transcript_support_level": null,
          "aa_start": 17,
          "aa_end": null,
          "aa_length": 194,
          "cds_start": 50,
          "cds_end": null,
          "cds_length": 585,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047424715.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.14+36A>G",
          "hgvs_p": null,
          "transcript": "NM_001145278.2",
          "protein_id": "NP_001138750.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 237,
          "cds_start": null,
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          "cds_length": 714,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145278.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "c.14+36A>G",
          "hgvs_p": null,
          "transcript": "ENST00000457722.6",
          "protein_id": "ENSP00000407091.2",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 237,
          "cds_start": null,
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          "cds_length": 714,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000457722.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "n.50A>G",
          "hgvs_p": null,
          "transcript": "ENST00000459640.5",
          "protein_id": "ENSP00000421335.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000459640.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "n.76A>G",
          "hgvs_p": null,
          "transcript": "ENST00000486390.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000486390.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "n.50A>G",
          "hgvs_p": null,
          "transcript": "ENST00000492095.5",
          "protein_id": "ENSP00000427620.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000492095.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "n.54A>G",
          "hgvs_p": null,
          "transcript": "ENST00000496239.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000496239.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
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          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "n.50A>G",
          "hgvs_p": null,
          "transcript": "ENST00000504858.5",
          "protein_id": "ENSP00000421867.1",
          "transcript_support_level": 5,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000504858.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "n.70A>G",
          "hgvs_p": null,
          "transcript": "ENST00000508680.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000508680.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAP2",
          "gene_hgnc_id": 25528,
          "hgvs_c": "n.38A>G",
          "hgvs_p": null,
          "transcript": "ENST00000513161.1",
          "protein_id": "ENSP00000423213.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000513161.1"
        }
      ],
      "gene_symbol": "NECAP2",
      "gene_hgnc_id": 25528,
      "dbsnp": "rs2086678716",
      "frequency_reference_population": 0.0000034202956,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.0000034203,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 5,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.41194796562194824,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.244,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.467,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.12,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.409,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001145277.2",
          "gene_symbol": "NECAP2",
          "hgnc_id": 25528,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.50A>G",
          "hgvs_p": "p.His17Arg"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}