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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-198253106-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=198253106&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 198253106,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_133494.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "NM_133494.3",
          "protein_id": "NP_598001.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000367385.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_133494.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000367385.9",
          "protein_id": "ENSP00000356355.4",
          "transcript_support_level": 5,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_133494.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000367385.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000538004.5",
          "protein_id": "ENSP00000444621.1",
          "transcript_support_level": 1,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000538004.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.154C>T",
          "hgvs_p": "p.Arg52Cys",
          "transcript": "ENST00000961625.1",
          "protein_id": "ENSP00000631684.1",
          "transcript_support_level": null,
          "aa_start": 52,
          "aa_end": null,
          "aa_length": 312,
          "cds_start": 154,
          "cds_end": null,
          "cds_length": 939,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961625.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876084.1",
          "protein_id": "ENSP00000546143.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 310,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 933,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876084.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000961630.1",
          "protein_id": "ENSP00000631689.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 310,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 933,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961630.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876079.1",
          "protein_id": "ENSP00000546138.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876079.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876085.1",
          "protein_id": "ENSP00000546144.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876085.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876086.1",
          "protein_id": "ENSP00000546145.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876086.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876087.1",
          "protein_id": "ENSP00000546146.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876087.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876088.1",
          "protein_id": "ENSP00000546147.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876088.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876093.1",
          "protein_id": "ENSP00000546152.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876093.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876102.1",
          "protein_id": "ENSP00000546161.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
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          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876102.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000961628.1",
          "protein_id": "ENSP00000631687.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000961628.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000961634.1",
          "protein_id": "ENSP00000631693.1",
          "transcript_support_level": null,
          "aa_start": 42,
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          "aa_length": 302,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961634.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000961640.1",
          "protein_id": "ENSP00000631699.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 124,
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          "cds_length": 909,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000961640.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000961641.1",
          "protein_id": "ENSP00000631700.1",
          "transcript_support_level": null,
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          "aa_length": 302,
          "cds_start": 124,
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        {
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          "intron_rank": null,
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          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
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          "protein_id": "ENSP00000631701.1",
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          "biotype": "protein_coding",
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        {
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          "intron_rank": null,
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          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.121C>T",
          "hgvs_p": "p.Arg41Cys",
          "transcript": "ENST00000876089.1",
          "protein_id": "ENSP00000546148.1",
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          "aa_start": 41,
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          "cds_start": 121,
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          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000876089.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEK7",
          "gene_hgnc_id": 13386,
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys",
          "transcript": "ENST00000876095.1",
          "protein_id": "ENSP00000546154.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 300,
          "cds_start": 124,
          "cds_end": null,
          "cds_length": 903,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876095.1"
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        {
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        {
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          "consequences": [
            "downstream_gene_variant"
          ],
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          "exon_count": 4,
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          "gene_symbol": "NEK7",
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          "hgvs_c": "c.*28C>T",
          "hgvs_p": null,
          "transcript": "ENST00000442588.5",
          "protein_id": "ENSP00000392745.1",
          "transcript_support_level": 5,
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          "cds_start": null,
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          "cds_length": 96,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000442588.5"
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      ],
      "gene_symbol": "NEK7",
      "gene_hgnc_id": 13386,
      "dbsnp": "rs761194915",
      "frequency_reference_population": 0.0000043434557,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 7,
      "gnomad_exomes_af": 0.00000411024,
      "gnomad_genomes_af": 0.00000658545,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7711296081542969,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.324,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.5278,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.19,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 3.516,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_133494.3",
          "gene_symbol": "NEK7",
          "hgnc_id": 13386,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.124C>T",
          "hgvs_p": "p.Arg42Cys"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}