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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-200553531-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=200553531&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 200553531,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_014875.3",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4804A>C",
          "hgvs_p": "p.Lys1602Gln",
          "transcript": "NM_014875.3",
          "protein_id": "NP_055690.1",
          "transcript_support_level": null,
          "aa_start": 1602,
          "aa_end": null,
          "aa_length": 1648,
          "cds_start": 4804,
          "cds_end": null,
          "cds_length": 4947,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000367350.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014875.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4804A>C",
          "hgvs_p": "p.Lys1602Gln",
          "transcript": "ENST00000367350.5",
          "protein_id": "ENSP00000356319.4",
          "transcript_support_level": 2,
          "aa_start": 1602,
          "aa_end": null,
          "aa_length": 1648,
          "cds_start": 4804,
          "cds_end": null,
          "cds_length": 4947,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_014875.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000367350.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4804A>C",
          "hgvs_p": "p.Lys1602Gln",
          "transcript": "ENST00000614960.4",
          "protein_id": "ENSP00000483069.1",
          "transcript_support_level": 1,
          "aa_start": 1602,
          "aa_end": null,
          "aa_length": 1648,
          "cds_start": 4804,
          "cds_end": null,
          "cds_length": 4947,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000614960.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4921A>C",
          "hgvs_p": "p.Lys1641Gln",
          "transcript": "ENST00000928797.1",
          "protein_id": "ENSP00000598856.1",
          "transcript_support_level": null,
          "aa_start": 1641,
          "aa_end": null,
          "aa_length": 1687,
          "cds_start": 4921,
          "cds_end": null,
          "cds_length": 5064,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928797.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4645A>C",
          "hgvs_p": "p.Lys1549Gln",
          "transcript": "ENST00000928795.1",
          "protein_id": "ENSP00000598854.1",
          "transcript_support_level": null,
          "aa_start": 1549,
          "aa_end": null,
          "aa_length": 1595,
          "cds_start": 4645,
          "cds_end": null,
          "cds_length": 4788,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928795.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4645A>C",
          "hgvs_p": "p.Lys1549Gln",
          "transcript": "ENST00000928796.1",
          "protein_id": "ENSP00000598855.1",
          "transcript_support_level": null,
          "aa_start": 1549,
          "aa_end": null,
          "aa_length": 1595,
          "cds_start": 4645,
          "cds_end": null,
          "cds_length": 4788,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928796.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.3331A>C",
          "hgvs_p": "p.Lys1111Gln",
          "transcript": "NM_001305792.1",
          "protein_id": "NP_001292721.1",
          "transcript_support_level": null,
          "aa_start": 1111,
          "aa_end": null,
          "aa_length": 1157,
          "cds_start": 3331,
          "cds_end": null,
          "cds_length": 3474,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001305792.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4804A>C",
          "hgvs_p": "p.Lys1602Gln",
          "transcript": "XM_011510231.3",
          "protein_id": "XP_011508533.1",
          "transcript_support_level": null,
          "aa_start": 1602,
          "aa_end": null,
          "aa_length": 1648,
          "cds_start": 4804,
          "cds_end": null,
          "cds_length": 4947,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011510231.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4804A>C",
          "hgvs_p": "p.Lys1602Gln",
          "transcript": "XM_011510232.3",
          "protein_id": "XP_011508534.1",
          "transcript_support_level": null,
          "aa_start": 1602,
          "aa_end": null,
          "aa_length": 1648,
          "cds_start": 4804,
          "cds_end": null,
          "cds_length": 4947,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011510232.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4720A>C",
          "hgvs_p": "p.Lys1574Gln",
          "transcript": "XM_011510233.3",
          "protein_id": "XP_011508535.1",
          "transcript_support_level": null,
          "aa_start": 1574,
          "aa_end": null,
          "aa_length": 1620,
          "cds_start": 4720,
          "cds_end": null,
          "cds_length": 4863,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011510233.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 32,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4720A>C",
          "hgvs_p": "p.Lys1574Gln",
          "transcript": "XM_047436181.1",
          "protein_id": "XP_047292137.1",
          "transcript_support_level": null,
          "aa_start": 1574,
          "aa_end": null,
          "aa_length": 1620,
          "cds_start": 4720,
          "cds_end": null,
          "cds_length": 4863,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436181.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4720A>C",
          "hgvs_p": "p.Lys1574Gln",
          "transcript": "XM_047436184.1",
          "protein_id": "XP_047292140.1",
          "transcript_support_level": null,
          "aa_start": 1574,
          "aa_end": null,
          "aa_length": 1620,
          "cds_start": 4720,
          "cds_end": null,
          "cds_length": 4863,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436184.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4720A>C",
          "hgvs_p": "p.Lys1574Gln",
          "transcript": "XM_047436190.1",
          "protein_id": "XP_047292146.1",
          "transcript_support_level": null,
          "aa_start": 1574,
          "aa_end": null,
          "aa_length": 1620,
          "cds_start": 4720,
          "cds_end": null,
          "cds_length": 4863,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436190.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4675A>C",
          "hgvs_p": "p.Lys1559Gln",
          "transcript": "XM_017003006.2",
          "protein_id": "XP_016858495.1",
          "transcript_support_level": null,
          "aa_start": 1559,
          "aa_end": null,
          "aa_length": 1605,
          "cds_start": 4675,
          "cds_end": null,
          "cds_length": 4818,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_017003006.2"
        },
        {
          "aa_ref": "K",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4591A>C",
          "hgvs_p": "p.Lys1531Gln",
          "transcript": "XM_047436195.1",
          "protein_id": "XP_047292151.1",
          "transcript_support_level": null,
          "aa_start": 1531,
          "aa_end": null,
          "aa_length": 1577,
          "cds_start": 4591,
          "cds_end": null,
          "cds_length": 4734,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436195.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4432A>C",
          "hgvs_p": "p.Lys1478Gln",
          "transcript": "XM_011510235.3",
          "protein_id": "XP_011508537.1",
          "transcript_support_level": null,
          "aa_start": 1478,
          "aa_end": null,
          "aa_length": 1524,
          "cds_start": 4432,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_011510235.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4432A>C",
          "hgvs_p": "p.Lys1478Gln",
          "transcript": "XM_047436197.1",
          "protein_id": "XP_047292153.1",
          "transcript_support_level": null,
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        },
        {
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          "protein_coding": true,
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          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
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          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4432A>C",
          "hgvs_p": "p.Lys1478Gln",
          "transcript": "XM_047436198.1",
          "protein_id": "XP_047292154.1",
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 31,
          "intron_rank": null,
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          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4303A>C",
          "hgvs_p": "p.Lys1435Gln",
          "transcript": "XM_047436199.1",
          "protein_id": "XP_047292155.1",
          "transcript_support_level": null,
          "aa_start": 1435,
          "aa_end": null,
          "aa_length": 1481,
          "cds_start": 4303,
          "cds_end": null,
          "cds_length": 4446,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436199.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KIF14",
          "gene_hgnc_id": 19181,
          "hgvs_c": "c.4237A>C",
          "hgvs_p": "p.Lys1413Gln",
          "transcript": "XM_017003007.2",
          "protein_id": "XP_016858496.1",
          "transcript_support_level": null,
          "aa_start": 1413,
          "aa_end": null,
          "aa_length": 1459,
          "cds_start": 4237,
          "cds_end": null,
          "cds_length": 4380,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017003007.2"
        }
      ],
      "gene_symbol": "KIF14",
      "gene_hgnc_id": 19181,
      "dbsnp": "rs776291323",
      "frequency_reference_population": 0.0000037175735,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 6,
      "gnomad_exomes_af": 0.0000027362,
      "gnomad_genomes_af": 0.0000131515,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.05583563446998596,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.09,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0933,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.62,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.484,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_014875.3",
          "gene_symbol": "KIF14",
          "hgnc_id": 19181,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.4804A>C",
          "hgvs_p": "p.Lys1602Gln"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}