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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-213005404-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=213005404&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 213005404,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_144567.5",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.763G>A",
          "hgvs_p": "p.Ala255Thr",
          "transcript": "NM_144567.5",
          "protein_id": "NP_653168.2",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000366962.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_144567.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.763G>A",
          "hgvs_p": "p.Ala255Thr",
          "transcript": "ENST00000366962.8",
          "protein_id": "ENSP00000355929.3",
          "transcript_support_level": 1,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_144567.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000366962.8"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.256G>A",
          "hgvs_p": "p.Ala86Thr",
          "transcript": "ENST00000360506.6",
          "protein_id": "ENSP00000353696.2",
          "transcript_support_level": 1,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 375,
          "cds_start": 256,
          "cds_end": null,
          "cds_length": 1128,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360506.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.256G>A",
          "hgvs_p": "p.Ala86Thr",
          "transcript": "ENST00000535388.2",
          "protein_id": "ENSP00000438141.2",
          "transcript_support_level": 1,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 375,
          "cds_start": 256,
          "cds_end": null,
          "cds_length": 1128,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000535388.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.763G>A",
          "hgvs_p": "p.Ala255Thr",
          "transcript": "ENST00000917618.1",
          "protein_id": "ENSP00000587677.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917618.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.385G>A",
          "hgvs_p": "p.Ala129Thr",
          "transcript": "NM_001300753.2",
          "protein_id": "NP_001287682.1",
          "transcript_support_level": null,
          "aa_start": 129,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 385,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001300753.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.385G>A",
          "hgvs_p": "p.Ala129Thr",
          "transcript": "NM_001300755.2",
          "protein_id": "NP_001287684.1",
          "transcript_support_level": null,
          "aa_start": 129,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 385,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001300755.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.331G>A",
          "hgvs_p": "p.Ala111Thr",
          "transcript": "ENST00000917619.1",
          "protein_id": "ENSP00000587678.1",
          "transcript_support_level": null,
          "aa_start": 111,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 331,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917619.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.256G>A",
          "hgvs_p": "p.Ala86Thr",
          "transcript": "NM_001300757.2",
          "protein_id": "NP_001287686.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 375,
          "cds_start": 256,
          "cds_end": null,
          "cds_length": 1128,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001300757.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.256G>A",
          "hgvs_p": "p.Ala86Thr",
          "transcript": "NM_001300758.2",
          "protein_id": "NP_001287687.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 375,
          "cds_start": 256,
          "cds_end": null,
          "cds_length": 1128,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001300758.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.697G>A",
          "hgvs_p": "p.Ala233Thr",
          "transcript": "XM_005273344.2",
          "protein_id": "XP_005273401.1",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 697,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005273344.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.697G>A",
          "hgvs_p": "p.Ala233Thr",
          "transcript": "XM_005273345.2",
          "protein_id": "XP_005273402.1",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 697,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005273345.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.697G>A",
          "hgvs_p": "p.Ala233Thr",
          "transcript": "XM_005273346.3",
          "protein_id": "XP_005273403.1",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 697,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005273346.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.697G>A",
          "hgvs_p": "p.Ala233Thr",
          "transcript": "XM_017002774.2",
          "protein_id": "XP_016858263.1",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 697,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017002774.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.526G>A",
          "hgvs_p": "p.Ala176Thr",
          "transcript": "XM_005273347.4",
          "protein_id": "XP_005273404.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 526,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005273347.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.385G>A",
          "hgvs_p": "p.Ala129Thr",
          "transcript": "XM_047433784.1",
          "protein_id": "XP_047289740.1",
          "transcript_support_level": null,
          "aa_start": 129,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 385,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047433784.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.763G>A",
          "hgvs_p": "p.Ala255Thr",
          "transcript": "XM_047433785.1",
          "protein_id": "XP_047289741.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047433785.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "c.385+7689G>A",
          "hgvs_p": null,
          "transcript": "ENST00000917617.1",
          "protein_id": "ENSP00000587676.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 187,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 564,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917617.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "n.634G>A",
          "hgvs_p": null,
          "transcript": "ENST00000476904.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000476904.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANGEL2",
          "gene_hgnc_id": 30534,
          "hgvs_c": "n.633G>A",
          "hgvs_p": null,
          "transcript": "NR_125333.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_125333.2"
        }
      ],
      "gene_symbol": "ANGEL2",
      "gene_hgnc_id": 30534,
      "dbsnp": "rs749390709",
      "frequency_reference_population": 0.000033469565,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 54,
      "gnomad_exomes_af": 0.0000342187,
      "gnomad_genomes_af": 0.0000262781,
      "gnomad_exomes_ac": 50,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9719594717025757,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.956,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.5915,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.57,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.476,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PP3_Strong"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_144567.5",
          "gene_symbol": "ANGEL2",
          "hgnc_id": 30534,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.763G>A",
          "hgvs_p": "p.Ala255Thr"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}