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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-21721716-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=21721716&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 21721716,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_032236.8",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
          "hgvs_p": "p.Arg566His",
          "transcript": "NM_032236.8",
          "protein_id": "NP_115612.4",
          "transcript_support_level": null,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 1035,
          "cds_start": 1697,
          "cds_end": null,
          "cds_length": 3108,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000308271.14",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032236.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
          "hgvs_p": "p.Arg566His",
          "transcript": "ENST00000308271.14",
          "protein_id": "ENSP00000309262.9",
          "transcript_support_level": 1,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 1035,
          "cds_start": 1697,
          "cds_end": null,
          "cds_length": 3108,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_032236.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000308271.14"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1694G>A",
          "hgvs_p": "p.Arg565His",
          "transcript": "ENST00000529637.5",
          "protein_id": "ENSP00000431949.1",
          "transcript_support_level": 1,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 1047,
          "cds_start": 1694,
          "cds_end": null,
          "cds_length": 3144,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000529637.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
          "hgvs_p": "p.Arg566His",
          "transcript": "ENST00000400301.5",
          "protein_id": "ENSP00000383157.1",
          "transcript_support_level": 1,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": 1697,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000400301.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
          "hgvs_p": "p.Arg566His",
          "transcript": "ENST00000903051.1",
          "protein_id": "ENSP00000573110.1",
          "transcript_support_level": null,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 1036,
          "cds_start": 1697,
          "cds_end": null,
          "cds_length": 3111,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903051.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1694G>A",
          "hgvs_p": "p.Arg565His",
          "transcript": "NM_001350167.2",
          "protein_id": "NP_001337096.1",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 1034,
          "cds_start": 1694,
          "cds_end": null,
          "cds_length": 3105,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350167.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
          "hgvs_p": "p.Arg566His",
          "transcript": "NM_001350168.2",
          "protein_id": "NP_001337097.1",
          "transcript_support_level": null,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 1034,
          "cds_start": 1697,
          "cds_end": null,
          "cds_length": 3105,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350168.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
          "hgvs_p": "p.Arg566His",
          "transcript": "ENST00000903043.1",
          "protein_id": "ENSP00000573102.1",
          "transcript_support_level": null,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 1034,
          "cds_start": 1697,
          "cds_end": null,
          "cds_length": 3105,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903043.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1694G>A",
          "hgvs_p": "p.Arg565His",
          "transcript": "ENST00000903045.1",
          "protein_id": "ENSP00000573104.1",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 1034,
          "cds_start": 1694,
          "cds_end": null,
          "cds_length": 3105,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903045.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1694G>A",
          "hgvs_p": "p.Arg565His",
          "transcript": "NM_001350166.2",
          "protein_id": "NP_001337095.1",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 1033,
          "cds_start": 1694,
          "cds_end": null,
          "cds_length": 3102,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001350166.2"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1694G>A",
          "hgvs_p": "p.Arg565His",
          "transcript": "ENST00000903046.1",
          "protein_id": "ENSP00000573105.1",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 1033,
          "cds_start": 1694,
          "cds_end": null,
          "cds_length": 3102,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
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          "consequences": [
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          ],
          "exon_rank": 13,
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          "intron_rank": null,
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          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
          "hgvs_p": "p.Arg566His",
          "transcript": "ENST00000963813.1",
          "protein_id": "ENSP00000633872.1",
          "transcript_support_level": null,
          "aa_start": 566,
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          "cds_start": 1697,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
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          "protein_coding": true,
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          "consequences": [
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          ],
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          "gene_symbol": "USP48",
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          "hgvs_c": "c.1697G>A",
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          "transcript": "ENST00000963818.1",
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        {
          "aa_ref": "R",
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          "strand": false,
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          ],
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
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          "transcript": "ENST00000922671.1",
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        {
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          "gene_symbol": "USP48",
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        {
          "aa_ref": "R",
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          "exon_count": 26,
          "intron_rank": null,
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          "gene_hgnc_id": 18533,
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          "transcript": "NM_001330394.3",
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        {
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          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1697G>A",
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          "transcript": "ENST00000903044.1",
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        {
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        {
          "aa_ref": "R",
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          "consequences": [
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          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP48",
          "gene_hgnc_id": 18533,
          "hgvs_c": "c.1499G>A",
          "hgvs_p": "p.Arg500His",
          "transcript": "ENST00000903048.1",
          "protein_id": "ENSP00000573107.1",
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000487880.2"
        }
      ],
      "gene_symbol": "USP48",
      "gene_hgnc_id": 18533,
      "dbsnp": "rs757075449",
      "frequency_reference_population": 0.000019893841,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 32,
      "gnomad_exomes_af": 0.0000212864,
      "gnomad_genomes_af": 0.00000656996,
      "gnomad_exomes_ac": 31,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.45916247367858887,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.151,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2344,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.05,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.236,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS2",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_032236.8",
          "gene_symbol": "USP48",
          "hgnc_id": 18533,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,Unknown",
          "hgvs_c": "c.1697G>A",
          "hgvs_p": "p.Arg566His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}