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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-237784175-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=237784175&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 237784175,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000366574.7",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 90,
          "exon_rank_end": null,
          "exon_count": 105,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12463T>A",
          "hgvs_p": "p.Ser4155Thr",
          "transcript": "NM_001035.3",
          "protein_id": "NP_001026.2",
          "transcript_support_level": null,
          "aa_start": 4155,
          "aa_end": null,
          "aa_length": 4967,
          "cds_start": 12463,
          "cds_end": null,
          "cds_length": 14904,
          "cdna_start": 12801,
          "cdna_end": null,
          "cdna_length": 16583,
          "mane_select": "ENST00000366574.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 90,
          "exon_rank_end": null,
          "exon_count": 105,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12463T>A",
          "hgvs_p": "p.Ser4155Thr",
          "transcript": "ENST00000366574.7",
          "protein_id": "ENSP00000355533.2",
          "transcript_support_level": 1,
          "aa_start": 4155,
          "aa_end": null,
          "aa_length": 4967,
          "cds_start": 12463,
          "cds_end": null,
          "cds_length": 14904,
          "cdna_start": 12801,
          "cdna_end": null,
          "cdna_length": 16583,
          "mane_select": "NM_001035.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 91,
          "exon_rank_end": null,
          "exon_count": 106,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12487T>A",
          "hgvs_p": "p.Ser4163Thr",
          "transcript": "ENST00000661330.2",
          "protein_id": "ENSP00000499393.2",
          "transcript_support_level": null,
          "aa_start": 4163,
          "aa_end": null,
          "aa_length": 4973,
          "cds_start": 12487,
          "cds_end": null,
          "cds_length": 14922,
          "cdna_start": 12825,
          "cdna_end": null,
          "cdna_length": 16601,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 89,
          "exon_rank_end": null,
          "exon_count": 104,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "n.*3555T>A",
          "hgvs_p": null,
          "transcript": "ENST00000609119.2",
          "protein_id": "ENSP00000499659.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 16525,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 89,
          "exon_rank_end": null,
          "exon_count": 104,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "n.*3555T>A",
          "hgvs_p": null,
          "transcript": "ENST00000609119.2",
          "protein_id": "ENSP00000499659.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 16525,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 91,
          "exon_rank_end": null,
          "exon_count": 106,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12484T>A",
          "hgvs_p": "p.Ser4162Thr",
          "transcript": "ENST00000660292.2",
          "protein_id": "ENSP00000499787.2",
          "transcript_support_level": null,
          "aa_start": 4162,
          "aa_end": null,
          "aa_length": 4974,
          "cds_start": 12484,
          "cds_end": null,
          "cds_length": 14925,
          "cdna_start": 12822,
          "cdna_end": null,
          "cdna_length": 16604,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 91,
          "exon_rank_end": null,
          "exon_count": 106,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12481T>A",
          "hgvs_p": "p.Ser4161Thr",
          "transcript": "ENST00000714021.1",
          "protein_id": "ENSP00000519311.1",
          "transcript_support_level": null,
          "aa_start": 4161,
          "aa_end": null,
          "aa_length": 4973,
          "cds_start": 12481,
          "cds_end": null,
          "cds_length": 14922,
          "cdna_start": 12819,
          "cdna_end": null,
          "cdna_length": 16601,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 90,
          "exon_rank_end": null,
          "exon_count": 105,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12451T>A",
          "hgvs_p": "p.Ser4151Thr",
          "transcript": "ENST00000659194.3",
          "protein_id": "ENSP00000499653.3",
          "transcript_support_level": null,
          "aa_start": 4151,
          "aa_end": null,
          "aa_length": 4961,
          "cds_start": 12451,
          "cds_end": null,
          "cds_length": 14886,
          "cdna_start": 12789,
          "cdna_end": null,
          "cdna_length": 16565,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 88,
          "exon_rank_end": null,
          "exon_count": 103,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12427T>A",
          "hgvs_p": "p.Ser4143Thr",
          "transcript": "ENST00000714019.1",
          "protein_id": "ENSP00000519309.1",
          "transcript_support_level": null,
          "aa_start": 4143,
          "aa_end": null,
          "aa_length": 4955,
          "cds_start": 12427,
          "cds_end": null,
          "cds_length": 14868,
          "cdna_start": 12765,
          "cdna_end": null,
          "cdna_length": 16547,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 87,
          "exon_rank_end": null,
          "exon_count": 102,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12352T>A",
          "hgvs_p": "p.Ser4118Thr",
          "transcript": "ENST00000714018.1",
          "protein_id": "ENSP00000519308.1",
          "transcript_support_level": null,
          "aa_start": 4118,
          "aa_end": null,
          "aa_length": 4930,
          "cds_start": 12352,
          "cds_end": null,
          "cds_length": 14793,
          "cdna_start": 12690,
          "cdna_end": null,
          "cdna_length": 16472,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 74,
          "exon_rank_end": null,
          "exon_count": 89,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.10498T>A",
          "hgvs_p": "p.Ser3500Thr",
          "transcript": "ENST00000714022.1",
          "protein_id": "ENSP00000519312.1",
          "transcript_support_level": null,
          "aa_start": 3500,
          "aa_end": null,
          "aa_length": 4312,
          "cds_start": 10498,
          "cds_end": null,
          "cds_length": 12939,
          "cdna_start": 10930,
          "cdna_end": null,
          "cdna_length": 14712,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 92,
          "exon_rank_end": null,
          "exon_count": 107,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12517T>A",
          "hgvs_p": "p.Ser4173Thr",
          "transcript": "XM_006711802.4",
          "protein_id": "XP_006711865.1",
          "transcript_support_level": null,
          "aa_start": 4173,
          "aa_end": null,
          "aa_length": 4985,
          "cds_start": 12517,
          "cds_end": null,
          "cds_length": 14958,
          "cdna_start": 12855,
          "cdna_end": null,
          "cdna_length": 16637,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 92,
          "exon_rank_end": null,
          "exon_count": 107,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12514T>A",
          "hgvs_p": "p.Ser4172Thr",
          "transcript": "XM_006711803.4",
          "protein_id": "XP_006711866.1",
          "transcript_support_level": null,
          "aa_start": 4172,
          "aa_end": null,
          "aa_length": 4984,
          "cds_start": 12514,
          "cds_end": null,
          "cds_length": 14955,
          "cdna_start": 12852,
          "cdna_end": null,
          "cdna_length": 16634,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 91,
          "exon_rank_end": null,
          "exon_count": 106,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12496T>A",
          "hgvs_p": "p.Ser4166Thr",
          "transcript": "XM_017002028.2",
          "protein_id": "XP_016857517.1",
          "transcript_support_level": null,
          "aa_start": 4166,
          "aa_end": null,
          "aa_length": 4978,
          "cds_start": 12496,
          "cds_end": null,
          "cds_length": 14937,
          "cdna_start": 12834,
          "cdna_end": null,
          "cdna_length": 16616,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 91,
          "exon_rank_end": null,
          "exon_count": 106,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12493T>A",
          "hgvs_p": "p.Ser4165Thr",
          "transcript": "XM_006711804.4",
          "protein_id": "XP_006711867.1",
          "transcript_support_level": null,
          "aa_start": 4165,
          "aa_end": null,
          "aa_length": 4977,
          "cds_start": 12493,
          "cds_end": null,
          "cds_length": 14934,
          "cdna_start": 12831,
          "cdna_end": null,
          "cdna_length": 16613,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 91,
          "exon_rank_end": null,
          "exon_count": 106,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12487T>A",
          "hgvs_p": "p.Ser4163Thr",
          "transcript": "XM_006711805.4",
          "protein_id": "XP_006711868.1",
          "transcript_support_level": null,
          "aa_start": 4163,
          "aa_end": null,
          "aa_length": 4975,
          "cds_start": 12487,
          "cds_end": null,
          "cds_length": 14928,
          "cdna_start": 12825,
          "cdna_end": null,
          "cdna_length": 16607,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 91,
          "exon_rank_end": null,
          "exon_count": 106,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12484T>A",
          "hgvs_p": "p.Ser4162Thr",
          "transcript": "XM_047427317.1",
          "protein_id": "XP_047283273.1",
          "transcript_support_level": null,
          "aa_start": 4162,
          "aa_end": null,
          "aa_length": 4974,
          "cds_start": 12484,
          "cds_end": null,
          "cds_length": 14925,
          "cdna_start": 12822,
          "cdna_end": null,
          "cdna_length": 16604,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 91,
          "exon_rank_end": null,
          "exon_count": 106,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12481T>A",
          "hgvs_p": "p.Ser4161Thr",
          "transcript": "XM_006711806.4",
          "protein_id": "XP_006711869.1",
          "transcript_support_level": null,
          "aa_start": 4161,
          "aa_end": null,
          "aa_length": 4973,
          "cds_start": 12481,
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          "cdna_start": 12819,
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          "cdna_length": 16601,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 90,
          "exon_rank_end": null,
          "exon_count": 105,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12457T>A",
          "hgvs_p": "p.Ser4153Thr",
          "transcript": "XM_006711807.4",
          "protein_id": "XP_006711870.1",
          "transcript_support_level": null,
          "aa_start": 4153,
          "aa_end": null,
          "aa_length": 4965,
          "cds_start": 12457,
          "cds_end": null,
          "cds_length": 14898,
          "cdna_start": 12795,
          "cdna_end": null,
          "cdna_length": 16577,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 89,
          "exon_rank_end": null,
          "exon_count": 104,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RYR2",
          "gene_hgnc_id": 10484,
          "hgvs_c": "c.12424T>A",
          "hgvs_p": "p.Ser4142Thr",
          "transcript": "XM_006711810.4",
          "protein_id": "XP_006711873.1",
          "transcript_support_level": null,
          "aa_start": 4142,
          "aa_end": null,
          "aa_length": 4954,
          "cds_start": 12424,
          "cds_end": null,
          "cds_length": 14865,
          "cdna_start": 12762,
          "cdna_end": null,
          "cdna_length": 16544,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 89,
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        },
        {
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      ],
      "gene_symbol": "RYR2",
      "gene_hgnc_id": 10484,
      "dbsnp": "rs1060500135",
      "frequency_reference_population": 0.0000013682792,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000136828,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5615618228912354,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.59,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1088,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.04,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 5.031,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP2,PP5",
      "acmg_by_gene": [
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PM1",
            "PM2",
            "PM5",
            "PP2",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000366574.7",
          "gene_symbol": "RYR2",
          "hgnc_id": 10484,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.12463T>A",
          "hgvs_p": "p.Ser4155Thr"
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000741291.1",
          "gene_symbol": "ENSG00000296715",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.641A>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Arrhythmogenic right ventricular dysplasia 2,Cardiovascular phenotype,Catecholaminergic polymorphic ventricular tachycardia 1,Ventricular arrhythmias due to cardiac ryanodine receptor calcium release deficiency syndrome,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:1 US:3",
      "phenotype_combined": "not specified|Ventricular arrhythmias due to cardiac ryanodine receptor calcium release deficiency syndrome;Arrhythmogenic right ventricular dysplasia 2;Catecholaminergic polymorphic ventricular tachycardia 1|Catecholaminergic polymorphic ventricular tachycardia 1|Cardiovascular phenotype",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}