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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-2406561-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=2406561&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 2406561,
      "ref": "C",
      "alt": "A",
      "effect": "stop_gained",
      "transcript": "ENST00000447513.7",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.835G>T",
          "hgvs_p": "p.Glu279*",
          "transcript": "NM_002617.4",
          "protein_id": "NP_002608.1",
          "transcript_support_level": null,
          "aa_start": 279,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 835,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": 897,
          "cdna_end": null,
          "cdna_length": 2835,
          "mane_select": "ENST00000447513.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.835G>T",
          "hgvs_p": "p.Glu279*",
          "transcript": "ENST00000447513.7",
          "protein_id": "ENSP00000407922.2",
          "transcript_support_level": 1,
          "aa_start": 279,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 835,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": 897,
          "cdna_end": null,
          "cdna_length": 2835,
          "mane_select": "NM_002617.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.895G>T",
          "hgvs_p": "p.Glu299*",
          "transcript": "ENST00000288774.8",
          "protein_id": "ENSP00000288774.3",
          "transcript_support_level": 1,
          "aa_start": 299,
          "aa_end": null,
          "aa_length": 346,
          "cds_start": 895,
          "cds_end": null,
          "cds_length": 1041,
          "cdna_start": 957,
          "cdna_end": null,
          "cdna_length": 2905,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.895G>T",
          "hgvs_p": "p.Glu299*",
          "transcript": "NM_153818.2",
          "protein_id": "NP_722540.1",
          "transcript_support_level": null,
          "aa_start": 299,
          "aa_end": null,
          "aa_length": 346,
          "cds_start": 895,
          "cds_end": null,
          "cds_length": 1041,
          "cdna_start": 957,
          "cdna_end": null,
          "cdna_length": 2895,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.892G>T",
          "hgvs_p": "p.Glu298*",
          "transcript": "NM_001374425.1",
          "protein_id": "NP_001361354.1",
          "transcript_support_level": null,
          "aa_start": 298,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 892,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": 954,
          "cdna_end": null,
          "cdna_length": 2892,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.835G>T",
          "hgvs_p": "p.Glu279*",
          "transcript": "ENST00000507596.5",
          "protein_id": "ENSP00000424291.1",
          "transcript_support_level": 5,
          "aa_start": 279,
          "aa_end": null,
          "aa_length": 324,
          "cds_start": 835,
          "cds_end": null,
          "cds_length": 975,
          "cdna_start": 873,
          "cdna_end": null,
          "cdna_length": 1941,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.460G>T",
          "hgvs_p": "p.Glu154*",
          "transcript": "NM_001374426.1",
          "protein_id": "NP_001361355.1",
          "transcript_support_level": null,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 201,
          "cds_start": 460,
          "cds_end": null,
          "cds_length": 606,
          "cdna_start": 1011,
          "cdna_end": null,
          "cdna_length": 2949,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.403G>T",
          "hgvs_p": "p.Glu135*",
          "transcript": "NM_001374427.1",
          "protein_id": "NP_001361356.1",
          "transcript_support_level": null,
          "aa_start": 135,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 403,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": 954,
          "cdna_end": null,
          "cdna_length": 2892,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "n.*201G>T",
          "hgvs_p": null,
          "transcript": "ENST00000510434.1",
          "protein_id": "ENSP00000423051.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 907,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "n.787G>T",
          "hgvs_p": null,
          "transcript": "ENST00000650293.1",
          "protein_id": "ENSP00000497980.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2260,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "n.950G>T",
          "hgvs_p": null,
          "transcript": "NR_164636.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2888,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "n.*201G>T",
          "hgvs_p": null,
          "transcript": "ENST00000510434.1",
          "protein_id": "ENSP00000423051.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 907,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.*301G>T",
          "hgvs_p": null,
          "transcript": "XM_047422539.1",
          "protein_id": "XP_047278495.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 209,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 630,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 831,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "PEX10",
      "gene_hgnc_id": 8851,
      "dbsnp": "rs62641225",
      "frequency_reference_population": 0.0000020533712,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205337,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5099999904632568,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.51,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 3.614,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000447513.7",
          "gene_symbol": "PEX10",
          "hgnc_id": 8851,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.835G>T",
          "hgvs_p": "p.Glu279*"
        }
      ],
      "clinvar_disease": " complementation group 7,Peroxisome biogenesis disorder,Peroxisome biogenesis disorder 6A (Zellweger),Zellweger spectrum disorders,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:3 LP:1",
      "phenotype_combined": "not provided|Peroxisome biogenesis disorder, complementation group 7|Zellweger spectrum disorders|Peroxisome biogenesis disorder 6A (Zellweger)|Peroxisome biogenesis disorder",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}