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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-2406576-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=2406576&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 2406576,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_153818.2",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.820A>G",
          "hgvs_p": "p.Thr274Ala",
          "transcript": "NM_002617.4",
          "protein_id": "NP_002608.1",
          "transcript_support_level": null,
          "aa_start": 274,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 820,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000447513.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002617.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.820A>G",
          "hgvs_p": "p.Thr274Ala",
          "transcript": "ENST00000447513.7",
          "protein_id": "ENSP00000407922.2",
          "transcript_support_level": 1,
          "aa_start": 274,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 820,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002617.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000447513.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.880A>G",
          "hgvs_p": "p.Thr294Ala",
          "transcript": "ENST00000288774.8",
          "protein_id": "ENSP00000288774.3",
          "transcript_support_level": 1,
          "aa_start": 294,
          "aa_end": null,
          "aa_length": 346,
          "cds_start": 880,
          "cds_end": null,
          "cds_length": 1041,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000288774.8"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.880A>G",
          "hgvs_p": "p.Thr294Ala",
          "transcript": "NM_153818.2",
          "protein_id": "NP_722540.1",
          "transcript_support_level": null,
          "aa_start": 294,
          "aa_end": null,
          "aa_length": 346,
          "cds_start": 880,
          "cds_end": null,
          "cds_length": 1041,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_153818.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.877A>G",
          "hgvs_p": "p.Thr293Ala",
          "transcript": "NM_001374425.1",
          "protein_id": "NP_001361354.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001374425.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.877A>G",
          "hgvs_p": "p.Thr293Ala",
          "transcript": "ENST00000874692.1",
          "protein_id": "ENSP00000544751.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874692.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.820A>G",
          "hgvs_p": "p.Thr274Ala",
          "transcript": "ENST00000507596.5",
          "protein_id": "ENSP00000424291.1",
          "transcript_support_level": 5,
          "aa_start": 274,
          "aa_end": null,
          "aa_length": 324,
          "cds_start": 820,
          "cds_end": null,
          "cds_length": 975,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000507596.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.802A>G",
          "hgvs_p": "p.Thr268Ala",
          "transcript": "ENST00000874693.1",
          "protein_id": "ENSP00000544752.1",
          "transcript_support_level": null,
          "aa_start": 268,
          "aa_end": null,
          "aa_length": 320,
          "cds_start": 802,
          "cds_end": null,
          "cds_length": 963,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874693.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.445A>G",
          "hgvs_p": "p.Thr149Ala",
          "transcript": "NM_001374426.1",
          "protein_id": "NP_001361355.1",
          "transcript_support_level": null,
          "aa_start": 149,
          "aa_end": null,
          "aa_length": 201,
          "cds_start": 445,
          "cds_end": null,
          "cds_length": 606,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001374426.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.388A>G",
          "hgvs_p": "p.Thr130Ala",
          "transcript": "NM_001374427.1",
          "protein_id": "NP_001361356.1",
          "transcript_support_level": null,
          "aa_start": 130,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 388,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001374427.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "n.*186A>G",
          "hgvs_p": null,
          "transcript": "ENST00000510434.1",
          "protein_id": "ENSP00000423051.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000510434.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "n.772A>G",
          "hgvs_p": null,
          "transcript": "ENST00000650293.1",
          "protein_id": "ENSP00000497980.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000650293.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "n.935A>G",
          "hgvs_p": null,
          "transcript": "NR_164636.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_164636.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "n.*186A>G",
          "hgvs_p": null,
          "transcript": "ENST00000510434.1",
          "protein_id": "ENSP00000423051.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000510434.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PEX10",
          "gene_hgnc_id": 8851,
          "hgvs_c": "c.*286A>G",
          "hgvs_p": null,
          "transcript": "XM_047422539.1",
          "protein_id": "XP_047278495.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 209,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 630,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047422539.1"
        }
      ],
      "gene_symbol": "PEX10",
      "gene_hgnc_id": 8851,
      "dbsnp": "rs34154371",
      "frequency_reference_population": 0.02272189,
      "hom_count_reference_population": 507,
      "allele_count_reference_population": 36655,
      "gnomad_exomes_af": 0.0231376,
      "gnomad_genomes_af": 0.01873,
      "gnomad_exomes_ac": 33805,
      "gnomad_genomes_ac": 2850,
      "gnomad_exomes_homalt": 465,
      "gnomad_genomes_homalt": 42,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.010609060525894165,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.357,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1091,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.29,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.96,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -18,
      "acmg_classification": "Benign",
      "acmg_criteria": "PM1,BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -18,
          "benign_score": 20,
          "pathogenic_score": 2,
          "criteria": [
            "PM1",
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_153818.2",
          "gene_symbol": "PEX10",
          "hgnc_id": 8851,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.880A>G",
          "hgvs_p": "p.Thr294Ala"
        }
      ],
      "clinvar_disease": " complementation group 7,Peroxisome biogenesis disorder,Peroxisome biogenesis disorder 6A (Zellweger),Zellweger spectrum disorders,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:3 B:5",
      "phenotype_combined": "not specified|Peroxisome biogenesis disorder, complementation group 7|Peroxisome biogenesis disorder 6A (Zellweger)|not provided|Zellweger spectrum disorders",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
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