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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-25301552-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=25301552&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 25301552,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000328664.9",
      "consequences": [
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "NM_016124.6",
          "protein_id": "NP_057208.3",
          "transcript_support_level": null,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 706,
          "cdna_end": null,
          "cdna_length": 2814,
          "mane_select": "ENST00000328664.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "ENST00000328664.9",
          "protein_id": "ENSP00000331871.4",
          "transcript_support_level": 1,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 706,
          "cdna_end": null,
          "cdna_length": 2814,
          "mane_select": "NM_016124.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "ENST00000342055.9",
          "protein_id": "ENSP00000339577.5",
          "transcript_support_level": 1,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 493,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1482,
          "cdna_start": 667,
          "cdna_end": null,
          "cdna_length": 1557,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "ENST00000568195.5",
          "protein_id": "ENSP00000456966.1",
          "transcript_support_level": 1,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": 667,
          "cdna_end": null,
          "cdna_length": 1467,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "ENST00000423810.6",
          "protein_id": "ENSP00000399640.2",
          "transcript_support_level": 1,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 431,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1296,
          "cdna_start": 667,
          "cdna_end": null,
          "cdna_length": 1637,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "ENST00000357542.8",
          "protein_id": "ENSP00000350150.4",
          "transcript_support_level": 1,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 396,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1191,
          "cdna_start": 667,
          "cdna_end": null,
          "cdna_length": 1387,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "ENST00000417538.6",
          "protein_id": "ENSP00000396420.2",
          "transcript_support_level": 1,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": 667,
          "cdna_end": null,
          "cdna_length": 1333,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "ENST00000454452.6",
          "protein_id": "ENSP00000413849.2",
          "transcript_support_level": 1,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": 717,
          "cdna_end": null,
          "cdna_length": 1303,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "n.822T>C",
          "hgvs_p": null,
          "transcript": "ENST00000564398.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1441,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "NM_001282871.2",
          "protein_id": "NP_001269800.1",
          "transcript_support_level": null,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 493,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1482,
          "cdna_start": 706,
          "cdna_end": null,
          "cdna_length": 2830,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 5,
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "NM_001282870.1",
          "protein_id": "NP_001269799.1",
          "transcript_support_level": null,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 667,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": 729,
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          "cdna_length": 2763,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "F",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "NM_001282872.1",
          "protein_id": "NP_001269801.1",
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          "cds_start": 667,
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          "mane_select": null,
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        },
        {
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 5,
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          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "RHD",
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          "hgvs_c": "c.667T>C",
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          "transcript": "ENST00000622561.4",
          "protein_id": "ENSP00000478087.1",
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          "cdna_start": 729,
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        },
        {
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          "protein_coding": true,
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          "consequences": [
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          ],
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "NM_001282869.2",
          "protein_id": "NP_001269798.1",
          "transcript_support_level": null,
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          "cds_start": 667,
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          "cdna_length": 2660,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "F",
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          ],
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          "gene_symbol": "RHD",
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        {
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          "consequences": [
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          ],
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "ENST00000648012.1",
          "protein_id": "ENSP00000498055.1",
          "transcript_support_level": null,
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        {
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          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": 5,
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          "exon_count": 7,
          "intron_rank": null,
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          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
          "transcript": "NM_001127691.3",
          "protein_id": "NP_001121163.1",
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          "cdna_length": 2526,
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        },
        {
          "aa_ref": "F",
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          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
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          "transcript": "NM_001282867.1",
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        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "c.667T>C",
          "hgvs_p": "p.Phe223Leu",
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        },
        {
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHD",
          "gene_hgnc_id": 10009,
          "hgvs_c": "n.667T>C",
          "hgvs_p": null,
          "transcript": "ENST00000423253.1",
          "protein_id": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 951,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RSRP1",
          "gene_hgnc_id": 25234,
          "hgvs_c": "c.-67+35488A>G",
          "hgvs_p": null,
          "transcript": "NM_001321772.2",
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      ],
      "gene_symbol": "RHD",
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      "dbsnp": "rs1053356",
      "frequency_reference_population": 8.018899e-7,
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      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 8.0189e-7,
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      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9411599636077881,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.6578,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.01,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 3.407,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
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            "PP3_Strong"
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          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000328664.9",
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        {
          "score": 6,
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          "criteria": [
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            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001321772.2",
          "gene_symbol": "RSRP1",
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          "effects": [
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          ],
          "inheritance_mode": "AR",
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      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}