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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-25408711-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=25408711&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 25408711,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000294413.13",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "NM_020485.8",
          "protein_id": "NP_065231.4",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 346,
          "cdna_end": null,
          "cdna_length": 1571,
          "mane_select": "ENST00000294413.13",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "ENST00000294413.13",
          "protein_id": "ENSP00000294413.6",
          "transcript_support_level": 1,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 346,
          "cdna_end": null,
          "cdna_length": 1571,
          "mane_select": "NM_020485.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "ENST00000413854.5",
          "protein_id": "ENSP00000415417.2",
          "transcript_support_level": 1,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 372,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 1119,
          "cdna_start": 327,
          "cdna_end": null,
          "cdna_length": 1198,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "ENST00000349438.8",
          "protein_id": "ENSP00000334570.5",
          "transcript_support_level": 1,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 354,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 1065,
          "cdna_start": 326,
          "cdna_end": null,
          "cdna_length": 1235,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "ENST00000340849.8",
          "protein_id": "ENSP00000345084.4",
          "transcript_support_level": 1,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 267,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 804,
          "cdna_start": 316,
          "cdna_end": null,
          "cdna_length": 1087,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "ENST00000346452.8",
          "protein_id": "ENSP00000344485.4",
          "transcript_support_level": 1,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 266,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 801,
          "cdna_start": 313,
          "cdna_end": null,
          "cdna_length": 971,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "n.462C>T",
          "hgvs_p": null,
          "transcript": "ENST00000527187.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1441,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.259C>T",
          "hgvs_p": "p.Pro87Ser",
          "transcript": "ENST00000349320.7",
          "protein_id": "ENSP00000311185.4",
          "transcript_support_level": 2,
          "aa_start": 87,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 259,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": 648,
          "cdna_end": null,
          "cdna_length": 1810,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "NM_001330430.4",
          "protein_id": "NP_001317359.1",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 372,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 1119,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 1601,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "NM_138618.6",
          "protein_id": "NP_619524.5",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 354,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 1065,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 1547,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "NM_138617.5",
          "protein_id": "NP_619523.3",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 267,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 804,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 1286,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "NM_138616.5",
          "protein_id": "NP_619522.3",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 266,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 801,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 1228,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.412C>T",
          "hgvs_p": "p.Pro138Ser",
          "transcript": "XM_011541889.4",
          "protein_id": "XP_011540191.2",
          "transcript_support_level": null,
          "aa_start": 138,
          "aa_end": null,
          "aa_length": 452,
          "cds_start": 412,
          "cds_end": null,
          "cds_length": 1359,
          "cdna_start": 423,
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          "cdna_length": 1648,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "XM_006710810.4",
          "protein_id": "XP_006710873.1",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 425,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 1278,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 6076,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "XM_005245957.5",
          "protein_id": "XP_005246014.1",
          "transcript_support_level": null,
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          "cds_start": 307,
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          "cdna_start": 456,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser",
          "transcript": "XM_017002014.3",
          "protein_id": "XP_016857503.1",
          "transcript_support_level": null,
          "aa_start": 103,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": 307,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 1473,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.187C>T",
          "hgvs_p": "p.Pro63Ser",
          "transcript": "XM_011541888.4",
          "protein_id": "XP_011540190.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 187,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": 500,
          "cdna_end": null,
          "cdna_length": 1725,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "c.139C>T",
          "hgvs_p": "p.Pro47Ser",
          "transcript": "XM_047427028.1",
          "protein_id": "XP_047282984.1",
          "transcript_support_level": null,
          "aa_start": 47,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 139,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": 252,
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          "cdna_length": 1477,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "n.413C>T",
          "hgvs_p": null,
          "transcript": "ENST00000495048.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 592,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RHCE",
          "gene_hgnc_id": 10008,
          "hgvs_c": "n.149-5965C>T",
          "hgvs_p": null,
          "transcript": "ENST00000533771.1",
          "protein_id": "ENSP00000435401.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 581,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "RHCE",
      "gene_hgnc_id": 10008,
      "dbsnp": "rs676785",
      "frequency_reference_population": 0.00008208833,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 10,
      "gnomad_exomes_af": 0.0000396474,
      "gnomad_genomes_af": 0.0000820883,
      "gnomad_exomes_ac": 46,
      "gnomad_genomes_ac": 10,
      "gnomad_exomes_homalt": 13,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.028504371643066406,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.015,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0686,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.69,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -2.325,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000294413.13",
          "gene_symbol": "RHCE",
          "hgnc_id": 10008,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.307C>T",
          "hgvs_p": "p.Pro103Ser"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "not provided",
      "clinvar_review_status": "no classification provided",
      "clinvar_submissions_summary": "O:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "not provided",
      "custom_annotations": null
    }
  ],
  "message": null
}