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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-25972602-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=25972602&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 25972602,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_000437.4",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "NM_000437.4",
          "protein_id": "NP_000428.2",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1040,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 1126,
          "cdna_end": null,
          "cdna_length": 3487,
          "mane_select": "ENST00000374282.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000437.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000374282.8",
          "protein_id": "ENSP00000363400.3",
          "transcript_support_level": 1,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1040,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 1126,
          "cdna_end": null,
          "cdna_length": 3487,
          "mane_select": "NM_000437.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000374282.8"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000374284.5",
          "protein_id": "ENSP00000363402.1",
          "transcript_support_level": 1,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1040,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 1165,
          "cdna_end": null,
          "cdna_length": 2523,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000374284.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1061A>G",
          "hgvs_p": "p.Glu354Gly",
          "transcript": "ENST00000872706.1",
          "protein_id": "ENSP00000542765.1",
          "transcript_support_level": null,
          "aa_start": 354,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 1061,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": 1165,
          "cdna_end": null,
          "cdna_length": 2495,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872706.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1055A>G",
          "hgvs_p": "p.Glu352Gly",
          "transcript": "ENST00000872707.1",
          "protein_id": "ENSP00000542766.1",
          "transcript_support_level": null,
          "aa_start": 352,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 1055,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": 1159,
          "cdna_end": null,
          "cdna_length": 2488,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872707.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000872702.1",
          "protein_id": "ENSP00000542761.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1040,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 2846,
          "cdna_end": null,
          "cdna_length": 4176,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872702.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000872708.1",
          "protein_id": "ENSP00000542767.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1040,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 1114,
          "cdna_end": null,
          "cdna_length": 2418,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872708.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000872709.1",
          "protein_id": "ENSP00000542768.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1040,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 1397,
          "cdna_end": null,
          "cdna_length": 2701,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872709.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000872710.1",
          "protein_id": "ENSP00000542769.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1040,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 1425,
          "cdna_end": null,
          "cdna_length": 1993,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872710.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000931127.1",
          "protein_id": "ENSP00000601186.1",
          "transcript_support_level": null,
          "aa_start": 347,
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          "aa_length": 392,
          "cds_start": 1040,
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          "cds_length": 1179,
          "cdna_start": 1311,
          "cdna_end": null,
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          "mane_select": null,
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        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
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          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000931128.1",
          "protein_id": "ENSP00000601187.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1040,
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          "cdna_start": 1341,
          "cdna_end": null,
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          "mane_select": null,
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        {
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          "consequences": [
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          "intron_rank": null,
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          "gene_symbol": "PAFAH2",
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          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000942550.1",
          "protein_id": "ENSP00000612609.1",
          "transcript_support_level": null,
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          "cds_start": 1040,
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          "cdna_start": 1319,
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          "mane_select": null,
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        {
          "aa_ref": "E",
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          "strand": false,
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          ],
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          "gene_symbol": "PAFAH2",
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          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly",
          "transcript": "ENST00000942551.1",
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
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          ],
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          "intron_rank": null,
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          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1031A>G",
          "hgvs_p": "p.Glu344Gly",
          "transcript": "ENST00000872703.1",
          "protein_id": "ENSP00000542762.1",
          "transcript_support_level": null,
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        {
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          "intron_rank": null,
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          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000872705.1"
        },
        {
          "aa_ref": "E",
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          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.926A>G",
          "hgvs_p": "p.Glu309Gly",
          "transcript": "ENST00000872704.1",
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          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
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        {
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          "consequences": [
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          ],
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          "exon_count": 12,
          "intron_rank": null,
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          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.1112A>G",
          "hgvs_p": "p.Glu371Gly",
          "transcript": "XM_011541528.3",
          "protein_id": "XP_011539830.1",
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        {
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          ],
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          "gene_symbol": "PAFAH2",
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          "transcript": "XM_006710670.4",
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.677A>G",
          "hgvs_p": "p.Glu226Gly",
          "transcript": "XM_047421700.1",
          "protein_id": "XP_047277656.1",
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          "cds_length": 816,
          "cdna_start": 842,
          "cdna_end": null,
          "cdna_length": 3203,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047421700.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PAFAH2",
          "gene_hgnc_id": 8579,
          "hgvs_c": "c.677A>G",
          "hgvs_p": "p.Glu226Gly",
          "transcript": "XM_047421701.1",
          "protein_id": "XP_047277657.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 271,
          "cds_start": 677,
          "cds_end": null,
          "cds_length": 816,
          "cdna_start": 738,
          "cdna_end": null,
          "cdna_length": 3099,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047421701.1"
        }
      ],
      "gene_symbol": "PAFAH2",
      "gene_hgnc_id": 8579,
      "dbsnp": "rs746569022",
      "frequency_reference_population": 0.000003098358,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000273661,
      "gnomad_genomes_af": 0.00000657479,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6144111752510071,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.19,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0991,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.378,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_000437.4",
          "gene_symbol": "PAFAH2",
          "hgnc_id": 8579,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1040A>G",
          "hgvs_p": "p.Glu347Gly"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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