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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-27370658-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=27370658&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 27370658,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_003665.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.596G>A",
          "hgvs_p": "p.Arg199His",
          "transcript": "NM_003665.4",
          "protein_id": "NP_003656.2",
          "transcript_support_level": null,
          "aa_start": 199,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 596,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000270879.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003665.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.596G>A",
          "hgvs_p": "p.Arg199His",
          "transcript": "ENST00000270879.9",
          "protein_id": "ENSP00000270879.4",
          "transcript_support_level": 1,
          "aa_start": 199,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 596,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003665.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000270879.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.563G>A",
          "hgvs_p": "p.Arg188His",
          "transcript": "ENST00000354982.2",
          "protein_id": "ENSP00000347077.2",
          "transcript_support_level": 1,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000354982.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.827G>A",
          "hgvs_p": "p.Arg276His",
          "transcript": "ENST00000859507.1",
          "protein_id": "ENSP00000529566.1",
          "transcript_support_level": null,
          "aa_start": 276,
          "aa_end": null,
          "aa_length": 376,
          "cds_start": 827,
          "cds_end": null,
          "cds_length": 1131,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859507.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.794G>A",
          "hgvs_p": "p.Arg265His",
          "transcript": "ENST00000859506.1",
          "protein_id": "ENSP00000529565.1",
          "transcript_support_level": null,
          "aa_start": 265,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 794,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859506.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.677G>A",
          "hgvs_p": "p.Arg226His",
          "transcript": "ENST00000859504.1",
          "protein_id": "ENSP00000529563.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 677,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859504.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.644G>A",
          "hgvs_p": "p.Arg215His",
          "transcript": "ENST00000966013.1",
          "protein_id": "ENSP00000636072.1",
          "transcript_support_level": null,
          "aa_start": 215,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 644,
          "cds_end": null,
          "cds_length": 948,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966013.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.638G>A",
          "hgvs_p": "p.Arg213His",
          "transcript": "ENST00000859500.1",
          "protein_id": "ENSP00000529559.1",
          "transcript_support_level": null,
          "aa_start": 213,
          "aa_end": null,
          "aa_length": 313,
          "cds_start": 638,
          "cds_end": null,
          "cds_length": 942,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859500.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.593G>A",
          "hgvs_p": "p.Arg198His",
          "transcript": "ENST00000859503.1",
          "protein_id": "ENSP00000529562.1",
          "transcript_support_level": null,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 298,
          "cds_start": 593,
          "cds_end": null,
          "cds_length": 897,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859503.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.587G>A",
          "hgvs_p": "p.Arg196His",
          "transcript": "ENST00000859498.1",
          "protein_id": "ENSP00000529557.1",
          "transcript_support_level": null,
          "aa_start": 196,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 587,
          "cds_end": null,
          "cds_length": 891,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859498.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.587G>A",
          "hgvs_p": "p.Arg196His",
          "transcript": "ENST00000859505.1",
          "protein_id": "ENSP00000529564.1",
          "transcript_support_level": null,
          "aa_start": 196,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 587,
          "cds_end": null,
          "cds_length": 891,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859505.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.587G>A",
          "hgvs_p": "p.Arg196His",
          "transcript": "ENST00000966011.1",
          "protein_id": "ENSP00000636070.1",
          "transcript_support_level": null,
          "aa_start": 196,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 587,
          "cds_end": null,
          "cds_length": 891,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966011.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.566G>A",
          "hgvs_p": "p.Arg189His",
          "transcript": "ENST00000966014.1",
          "protein_id": "ENSP00000636073.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 289,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 870,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966014.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.563G>A",
          "hgvs_p": "p.Arg188His",
          "transcript": "NM_173452.3",
          "protein_id": "NP_775628.1",
          "transcript_support_level": null,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_173452.3"
        },
        {
          "aa_ref": "R",
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.563G>A",
          "hgvs_p": "p.Arg188His",
          "transcript": "ENST00000859501.1",
          "protein_id": "ENSP00000529560.1",
          "transcript_support_level": null,
          "aa_start": 188,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 563,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859501.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.554G>A",
          "hgvs_p": "p.Arg185His",
          "transcript": "ENST00000859499.1",
          "protein_id": "ENSP00000529558.1",
          "transcript_support_level": null,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 285,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 858,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000859499.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.551G>A",
          "hgvs_p": "p.Arg184His",
          "transcript": "ENST00000966012.1",
          "protein_id": "ENSP00000636071.1",
          "transcript_support_level": null,
          "aa_start": 184,
          "aa_end": null,
          "aa_length": 284,
          "cds_start": 551,
          "cds_end": null,
          "cds_length": 855,
          "cdna_start": null,
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          "mane_select": null,
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        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.530G>A",
          "hgvs_p": "p.Arg177His",
          "transcript": "ENST00000859502.1",
          "protein_id": "ENSP00000529561.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 277,
          "cds_start": 530,
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          "cds_length": 834,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000859502.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.518G>A",
          "hgvs_p": "p.Arg173His",
          "transcript": "ENST00000966010.1",
          "protein_id": "ENSP00000636069.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 518,
          "cds_end": null,
          "cds_length": 822,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966010.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "c.596G>A",
          "hgvs_p": "p.Arg199His",
          "transcript": "ENST00000699963.1",
          "protein_id": "ENSP00000514719.1",
          "transcript_support_level": null,
          "aa_start": 199,
          "aa_end": null,
          "aa_length": 221,
          "cds_start": 596,
          "cds_end": null,
          "cds_length": 666,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000699963.1"
        },
        {
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          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "n.746G>A",
          "hgvs_p": null,
          "transcript": "ENST00000498393.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000498393.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FCN3",
          "gene_hgnc_id": 3625,
          "hgvs_c": "n.595G>A",
          "hgvs_p": null,
          "transcript": "ENST00000699962.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000699962.1"
        }
      ],
      "gene_symbol": "FCN3",
      "gene_hgnc_id": 3625,
      "dbsnp": "rs546449247",
      "frequency_reference_population": 0.000042745316,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 69,
      "gnomad_exomes_af": 0.0000458311,
      "gnomad_genomes_af": 0.0000131301,
      "gnomad_exomes_ac": 67,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.1193133294582367,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.138,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0703,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.5,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.19,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_003665.4",
          "gene_symbol": "FCN3",
          "hgnc_id": 3625,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.596G>A",
          "hgvs_p": "p.Arg199His"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}