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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-27914145-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=27914145&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 27914145,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001297558.1",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.35C>T",
          "hgvs_p": "p.Ser12Phe",
          "transcript": "NM_002946.5",
          "protein_id": "NP_002937.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 270,
          "cds_start": 35,
          "cds_end": null,
          "cds_length": 813,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000373912.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002946.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.35C>T",
          "hgvs_p": "p.Ser12Phe",
          "transcript": "ENST00000373912.8",
          "protein_id": "ENSP00000363021.3",
          "transcript_support_level": 1,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 270,
          "cds_start": 35,
          "cds_end": null,
          "cds_length": 813,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002946.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000373912.8"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.299C>T",
          "hgvs_p": "p.Ser100Phe",
          "transcript": "ENST00000313433.11",
          "protein_id": "ENSP00000363015.3",
          "transcript_support_level": 1,
          "aa_start": 100,
          "aa_end": null,
          "aa_length": 358,
          "cds_start": 299,
          "cds_end": null,
          "cds_length": 1077,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000313433.11"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.80C>T",
          "hgvs_p": "p.Ser27Phe",
          "transcript": "ENST00000935486.1",
          "protein_id": "ENSP00000605545.1",
          "transcript_support_level": null,
          "aa_start": 27,
          "aa_end": null,
          "aa_length": 285,
          "cds_start": 80,
          "cds_end": null,
          "cds_length": 858,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935486.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.59C>T",
          "hgvs_p": "p.Ser20Phe",
          "transcript": "NM_001297558.1",
          "protein_id": "NP_001284487.1",
          "transcript_support_level": null,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 59,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001297558.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.59C>T",
          "hgvs_p": "p.Ser20Phe",
          "transcript": "ENST00000373909.7",
          "protein_id": "ENSP00000363017.3",
          "transcript_support_level": 3,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 278,
          "cds_start": 59,
          "cds_end": null,
          "cds_length": 837,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000373909.7"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.47C>T",
          "hgvs_p": "p.Ser16Phe",
          "transcript": "NM_001355129.2",
          "protein_id": "NP_001342058.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 274,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 825,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001355129.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.35C>T",
          "hgvs_p": "p.Ser12Phe",
          "transcript": "ENST00000882504.1",
          "protein_id": "ENSP00000552563.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 245,
          "cds_start": 35,
          "cds_end": null,
          "cds_length": 738,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882504.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.35C>T",
          "hgvs_p": "p.Ser12Phe",
          "transcript": "ENST00000882506.1",
          "protein_id": "ENSP00000552565.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 232,
          "cds_start": 35,
          "cds_end": null,
          "cds_length": 699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882506.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.47C>T",
          "hgvs_p": "p.Ser16Phe",
          "transcript": "ENST00000935485.1",
          "protein_id": "ENSP00000605544.1",
          "transcript_support_level": null,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 210,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 633,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935485.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.35C>T",
          "hgvs_p": "p.Ser12Phe",
          "transcript": "ENST00000882505.1",
          "protein_id": "ENSP00000552564.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 206,
          "cds_start": 35,
          "cds_end": null,
          "cds_length": 621,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882505.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.35C>T",
          "hgvs_p": "p.Ser12Phe",
          "transcript": "ENST00000882507.1",
          "protein_id": "ENSP00000552566.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 198,
          "cds_start": 35,
          "cds_end": null,
          "cds_length": 597,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882507.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.47C>T",
          "hgvs_p": "p.Ser16Phe",
          "transcript": "ENST00000444045.1",
          "protein_id": "ENSP00000387649.1",
          "transcript_support_level": 5,
          "aa_start": 16,
          "aa_end": null,
          "aa_length": 178,
          "cds_start": 47,
          "cds_end": null,
          "cds_length": 537,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000444045.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.59C>T",
          "hgvs_p": "p.Ser20Phe",
          "transcript": "XM_024448862.2",
          "protein_id": "XP_024304630.1",
          "transcript_support_level": null,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 253,
          "cds_start": 59,
          "cds_end": null,
          "cds_length": 762,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024448862.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.35C>T",
          "hgvs_p": "p.Ser12Phe",
          "transcript": "XM_024448864.2",
          "protein_id": "XP_024304632.1",
          "transcript_support_level": null,
          "aa_start": 12,
          "aa_end": null,
          "aa_length": 245,
          "cds_start": 35,
          "cds_end": null,
          "cds_length": 738,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024448864.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.-405C>T",
          "hgvs_p": null,
          "transcript": "NM_001286076.2",
          "protein_id": "NP_001273005.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 174,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 525,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286076.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RPA2",
          "gene_hgnc_id": 10290,
          "hgvs_c": "c.-172+289C>T",
          "hgvs_p": null,
          "transcript": "NM_001355128.2",
          "protein_id": "NP_001342057.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 174,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 525,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001355128.2"
        }
      ],
      "gene_symbol": "RPA2",
      "gene_hgnc_id": 10290,
      "dbsnp": "rs776471331",
      "frequency_reference_population": 0.000009296076,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 15,
      "gnomad_exomes_af": 0.00000889397,
      "gnomad_genomes_af": 0.0000131648,
      "gnomad_exomes_ac": 13,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.10454323887825012,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.084,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.3353,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.35,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.233,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001297558.1",
          "gene_symbol": "RPA2",
          "hgnc_id": 10290,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.59C>T",
          "hgvs_p": "p.Ser20Phe"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}