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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-32042554-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=32042554&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 32042554,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_006559.3",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1262C>T",
          "hgvs_p": "p.Pro421Leu",
          "transcript": "NM_006559.3",
          "protein_id": "NP_006550.1",
          "transcript_support_level": null,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1262,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000327300.12",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006559.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1262C>T",
          "hgvs_p": "p.Pro421Leu",
          "transcript": "ENST00000327300.12",
          "protein_id": "ENSP00000313829.7",
          "transcript_support_level": 1,
          "aa_start": 421,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1262,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_006559.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000327300.12"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1145C>T",
          "hgvs_p": "p.Pro382Leu",
          "transcript": "ENST00000492989.1",
          "protein_id": "ENSP00000417731.1",
          "transcript_support_level": 1,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 1145,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000492989.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "n.1415C>T",
          "hgvs_p": null,
          "transcript": "ENST00000307714.12",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000307714.12"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1349C>T",
          "hgvs_p": "p.Pro450Leu",
          "transcript": "ENST00000906251.1",
          "protein_id": "ENSP00000576310.1",
          "transcript_support_level": null,
          "aa_start": 450,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 1349,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906251.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1259C>T",
          "hgvs_p": "p.Pro420Leu",
          "transcript": "ENST00000916417.1",
          "protein_id": "ENSP00000586476.1",
          "transcript_support_level": null,
          "aa_start": 420,
          "aa_end": null,
          "aa_length": 442,
          "cds_start": 1259,
          "cds_end": null,
          "cds_length": 1329,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916417.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1256C>T",
          "hgvs_p": "p.Pro419Leu",
          "transcript": "ENST00000967223.1",
          "protein_id": "ENSP00000637282.1",
          "transcript_support_level": null,
          "aa_start": 419,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 1256,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967223.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1238C>T",
          "hgvs_p": "p.Pro413Leu",
          "transcript": "ENST00000906250.1",
          "protein_id": "ENSP00000576309.1",
          "transcript_support_level": null,
          "aa_start": 413,
          "aa_end": null,
          "aa_length": 435,
          "cds_start": 1238,
          "cds_end": null,
          "cds_length": 1308,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906250.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1145C>T",
          "hgvs_p": "p.Pro382Leu",
          "transcript": "NM_001271878.2",
          "protein_id": "NP_001258807.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 1145,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001271878.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.1094C>T",
          "hgvs_p": "p.Pro365Leu",
          "transcript": "ENST00000967224.1",
          "protein_id": "ENSP00000637283.1",
          "transcript_support_level": null,
          "aa_start": 365,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 1094,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967224.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.992C>T",
          "hgvs_p": "p.Pro331Leu",
          "transcript": "ENST00000916418.1",
          "protein_id": "ENSP00000586477.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 992,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916418.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Pro309Leu",
          "transcript": "ENST00000916419.1",
          "protein_id": "ENSP00000586478.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 331,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 996,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916419.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "c.875C>T",
          "hgvs_p": "p.Pro292Leu",
          "transcript": "ENST00000906249.1",
          "protein_id": "ENSP00000576308.1",
          "transcript_support_level": null,
          "aa_start": 292,
          "aa_end": null,
          "aa_length": 314,
          "cds_start": 875,
          "cds_end": null,
          "cds_length": 945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906249.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "n.1076C>T",
          "hgvs_p": null,
          "transcript": "ENST00000484270.2",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000484270.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "n.1473C>T",
          "hgvs_p": null,
          "transcript": "NR_073498.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_073498.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "n.1390C>T",
          "hgvs_p": null,
          "transcript": "NR_073499.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_073499.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KHDRBS1",
          "gene_hgnc_id": 18116,
          "hgvs_c": "n.1273C>T",
          "hgvs_p": null,
          "transcript": "XR_007066502.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007066502.1"
        }
      ],
      "gene_symbol": "KHDRBS1",
      "gene_hgnc_id": 18116,
      "dbsnp": "rs750291697",
      "frequency_reference_population": 0.000013014894,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 21,
      "gnomad_exomes_af": 0.0000123173,
      "gnomad_genomes_af": 0.000019714,
      "gnomad_exomes_ac": 18,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.17528024315834045,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.102,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2274,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.36,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.297,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP2,PP5_Moderate,BP4,BS2",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 5,
          "pathogenic_score": 3,
          "criteria": [
            "PP2",
            "PP5_Moderate",
            "BP4",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_006559.3",
          "gene_symbol": "KHDRBS1",
          "hgnc_id": 18116,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1262C>T",
          "hgvs_p": "p.Pro421Leu"
        }
      ],
      "clinvar_disease": "Premature ovarian failure",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Premature ovarian failure",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}