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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-32944087-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=32944087&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 32944087,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_153341.4",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1334G>A",
          "hgvs_p": "p.Cys445Tyr",
          "transcript": "NM_001300826.2",
          "protein_id": "NP_001287755.1",
          "transcript_support_level": null,
          "aa_start": 445,
          "aa_end": null,
          "aa_length": 731,
          "cds_start": 1334,
          "cds_end": null,
          "cds_length": 2196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000235150.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001300826.2"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1334G>A",
          "hgvs_p": "p.Cys445Tyr",
          "transcript": "ENST00000235150.5",
          "protein_id": "ENSP00000235150.4",
          "transcript_support_level": 1,
          "aa_start": 445,
          "aa_end": null,
          "aa_length": 731,
          "cds_start": 1334,
          "cds_end": null,
          "cds_length": 2196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001300826.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000235150.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Cys446Tyr",
          "transcript": "ENST00000373456.11",
          "protein_id": "ENSP00000362555.7",
          "transcript_support_level": 1,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 732,
          "cds_start": 1337,
          "cds_end": null,
          "cds_length": 2199,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000373456.11"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1334G>A",
          "hgvs_p": "p.Cys445Tyr",
          "transcript": "ENST00000356990.9",
          "protein_id": "ENSP00000349482.5",
          "transcript_support_level": 1,
          "aa_start": 445,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1334,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000356990.9"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1334G>A",
          "hgvs_p": "p.Cys445Tyr",
          "transcript": "ENST00000959794.1",
          "protein_id": "ENSP00000629853.1",
          "transcript_support_level": null,
          "aa_start": 445,
          "aa_end": null,
          "aa_length": 763,
          "cds_start": 1334,
          "cds_end": null,
          "cds_length": 2292,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959794.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1427G>A",
          "hgvs_p": "p.Cys476Tyr",
          "transcript": "ENST00000959797.1",
          "protein_id": "ENSP00000629856.1",
          "transcript_support_level": null,
          "aa_start": 476,
          "aa_end": null,
          "aa_length": 762,
          "cds_start": 1427,
          "cds_end": null,
          "cds_length": 2289,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959797.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Cys446Tyr",
          "transcript": "NM_153341.4",
          "protein_id": "NP_699172.2",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 732,
          "cds_start": 1337,
          "cds_end": null,
          "cds_length": 2199,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_153341.4"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1334G>A",
          "hgvs_p": "p.Cys445Tyr",
          "transcript": "ENST00000921592.1",
          "protein_id": "ENSP00000591651.1",
          "transcript_support_level": null,
          "aa_start": 445,
          "aa_end": null,
          "aa_length": 731,
          "cds_start": 1334,
          "cds_end": null,
          "cds_length": 2196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000921592.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Cys446Tyr",
          "transcript": "ENST00000959791.1",
          "protein_id": "ENSP00000629850.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 688,
          "cds_start": 1337,
          "cds_end": null,
          "cds_length": 2067,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959791.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Cys446Tyr",
          "transcript": "ENST00000959796.1",
          "protein_id": "ENSP00000629855.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 688,
          "cds_start": 1337,
          "cds_end": null,
          "cds_length": 2067,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959796.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1334G>A",
          "hgvs_p": "p.Cys445Tyr",
          "transcript": "ENST00000921593.1",
          "protein_id": "ENSP00000591652.1",
          "transcript_support_level": null,
          "aa_start": 445,
          "aa_end": null,
          "aa_length": 687,
          "cds_start": 1334,
          "cds_end": null,
          "cds_length": 2064,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000921593.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.986G>A",
          "hgvs_p": "p.Cys329Tyr",
          "transcript": "ENST00000959798.1",
          "protein_id": "ENSP00000629857.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1848,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959798.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1334G>A",
          "hgvs_p": "p.Cys445Tyr",
          "transcript": "NM_001127361.3",
          "protein_id": "NP_001120833.1",
          "transcript_support_level": null,
          "aa_start": 445,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1334,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001127361.3"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Cys446Tyr",
          "transcript": "XM_006710356.3",
          "protein_id": "XP_006710419.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 732,
          "cds_start": 1337,
          "cds_end": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "XM_006710356.3"
        },
        {
          "aa_ref": "C",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Cys446Tyr",
          "transcript": "XM_006710357.4",
          "protein_id": "XP_006710420.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 688,
          "cds_start": 1337,
          "cds_end": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_006710357.4"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1334G>A",
          "hgvs_p": "p.Cys445Tyr",
          "transcript": "XM_047445558.1",
          "protein_id": "XP_047301514.1",
          "transcript_support_level": null,
          "aa_start": 445,
          "aa_end": null,
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          "cds_start": 1334,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Cys446Tyr",
          "transcript": "XM_006710358.4",
          "protein_id": "XP_006710421.1",
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        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 8,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1264+1427G>A",
          "hgvs_p": null,
          "transcript": "ENST00000959795.1",
          "protein_id": "ENSP00000629854.1",
          "transcript_support_level": null,
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          "cds_start": null,
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        },
        {
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          "canonical": false,
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          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 9,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1261+1427G>A",
          "hgvs_p": null,
          "transcript": "ENST00000959790.1",
          "protein_id": "ENSP00000629849.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 684,
          "cds_start": null,
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          "cds_length": 2055,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959790.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "RNF19B",
          "gene_hgnc_id": 26886,
          "hgvs_c": "c.1261+1427G>A",
          "hgvs_p": null,
          "transcript": "ENST00000959793.1",
          "protein_id": "ENSP00000629852.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 684,
          "cds_start": null,
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        {
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        {
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          "gene_symbol": "ENSG00000287691",
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          "biotype": "pseudogene",
          "feature": "ENST00000838088.1"
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      ],
      "gene_symbol": "RNF19B",
      "gene_hgnc_id": 26886,
      "dbsnp": "rs1038997469",
      "frequency_reference_population": 0.00004337244,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 70,
      "gnomad_exomes_af": 0.0000465202,
      "gnomad_genomes_af": 0.0000131408,
      "gnomad_exomes_ac": 68,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8631466627120972,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.522,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9961,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.19,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.905,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_153341.4",
          "gene_symbol": "RNF19B",
          "hgnc_id": 26886,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Cys446Tyr"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000661031.1",
          "gene_symbol": "ENSG00000287691",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.364-7340C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}