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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-39331383-A-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=39331383&ref=A&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 39331383,
      "ref": "A",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000564288.6",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 37,
          "exon_rank_end": null,
          "exon_count": 101,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.4795A>T",
          "hgvs_p": "p.Ile1599Leu",
          "transcript": "NM_001394062.1",
          "protein_id": "NP_001380991.1",
          "transcript_support_level": null,
          "aa_start": 1599,
          "aa_end": null,
          "aa_length": 7555,
          "cds_start": 4795,
          "cds_end": null,
          "cds_length": 22668,
          "cdna_start": 5123,
          "cdna_end": null,
          "cdna_length": 24340,
          "mane_select": "ENST00000564288.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 37,
          "exon_rank_end": null,
          "exon_count": 101,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.4795A>T",
          "hgvs_p": "p.Ile1599Leu",
          "transcript": "ENST00000564288.6",
          "protein_id": "ENSP00000455274.1",
          "transcript_support_level": 5,
          "aa_start": 1599,
          "aa_end": null,
          "aa_length": 7555,
          "cds_start": 4795,
          "cds_end": null,
          "cds_length": 22668,
          "cdna_start": 5123,
          "cdna_end": null,
          "cdna_length": 24340,
          "mane_select": "NM_001394062.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 37,
          "exon_rank_end": null,
          "exon_count": 101,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.4906A>T",
          "hgvs_p": "p.Ile1636Leu",
          "transcript": "ENST00000567887.5",
          "protein_id": "ENSP00000455823.1",
          "transcript_support_level": 5,
          "aa_start": 1636,
          "aa_end": null,
          "aa_length": 7592,
          "cds_start": 4906,
          "cds_end": null,
          "cds_length": 22779,
          "cdna_start": 5063,
          "cdna_end": null,
          "cdna_length": 24319,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 96,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.4810A>T",
          "hgvs_p": "p.Ile1604Leu",
          "transcript": "ENST00000372915.8",
          "protein_id": "ENSP00000362006.4",
          "transcript_support_level": 5,
          "aa_start": 1604,
          "aa_end": null,
          "aa_length": 7385,
          "cds_start": 4810,
          "cds_end": null,
          "cds_length": 22158,
          "cdna_start": 4897,
          "cdna_end": null,
          "cdna_length": 23431,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.4642A>T",
          "hgvs_p": "p.Ile1548Leu",
          "transcript": "ENST00000672812.1",
          "protein_id": "ENSP00000499888.1",
          "transcript_support_level": null,
          "aa_start": 1548,
          "aa_end": null,
          "aa_length": 2145,
          "cds_start": 4642,
          "cds_end": null,
          "cds_length": 6440,
          "cdna_start": 4747,
          "cdna_end": null,
          "cdna_length": 6545,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "n.246A>T",
          "hgvs_p": null,
          "transcript": "ENST00000289893.8",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 19141,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "n.*269A>T",
          "hgvs_p": null,
          "transcript": "ENST00000673926.1",
          "protein_id": "ENSP00000501154.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5693,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "n.*269A>T",
          "hgvs_p": null,
          "transcript": "ENST00000673926.1",
          "protein_id": "ENSP00000501154.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5693,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 93,
          "intron_rank": 35,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.4629+4030A>T",
          "hgvs_p": null,
          "transcript": "NM_012090.5",
          "protein_id": "NP_036222.3",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 5430,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 16293,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 17689,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 94,
          "intron_rank": 36,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.4629+4030A>T",
          "hgvs_p": null,
          "transcript": "ENST00000361689.7",
          "protein_id": "ENSP00000354573.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 5430,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 16293,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 17665,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 93,
          "intron_rank": 35,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.4476+4030A>T",
          "hgvs_p": null,
          "transcript": "ENST00000671089.2",
          "protein_id": "ENSP00000499399.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 5379,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 16140,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 17433,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 71,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.2028+4030A>T",
          "hgvs_p": null,
          "transcript": "ENST00000372925.6",
          "protein_id": "ENSP00000362016.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 4433,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 13302,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 14496,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": 31,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "c.5076+4030A>T",
          "hgvs_p": null,
          "transcript": "ENST00000530262.5",
          "protein_id": "ENSP00000437059.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 2027,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 6086,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6257,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "n.2029+4030A>T",
          "hgvs_p": null,
          "transcript": "ENST00000476350.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5304,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MACF1",
          "gene_hgnc_id": 13664,
          "hgvs_c": "n.*139A>T",
          "hgvs_p": null,
          "transcript": "ENST00000528611.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 380,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "MACF1",
      "gene_hgnc_id": 13664,
      "dbsnp": "rs16826069",
      "frequency_reference_population": 6.840703e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.8407e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.027591466903686523,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.133,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0491,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.58,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.098,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000564288.6",
          "gene_symbol": "MACF1",
          "hgnc_id": 13664,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.4795A>T",
          "hgvs_p": "p.Ile1599Leu"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}