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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-43993930-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=43993930&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 43993930,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001349128.1",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.463G>A",
          "hgvs_p": "p.Val155Met",
          "transcript": "NM_152499.4",
          "protein_id": "NP_689712.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000372318.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_152499.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.463G>A",
          "hgvs_p": "p.Val155Met",
          "transcript": "ENST00000372318.8",
          "protein_id": "ENSP00000361392.3",
          "transcript_support_level": 1,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_152499.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000372318.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "n.167G>A",
          "hgvs_p": null,
          "transcript": "ENST00000463846.5",
          "protein_id": "ENSP00000476322.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000463846.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "n.830G>A",
          "hgvs_p": null,
          "transcript": "ENST00000490563.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000490563.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.571G>A",
          "hgvs_p": "p.Val191Met",
          "transcript": "ENST00000857687.1",
          "protein_id": "ENSP00000527746.1",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 571,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857687.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.571G>A",
          "hgvs_p": "p.Val191Met",
          "transcript": "ENST00000968713.1",
          "protein_id": "ENSP00000638772.1",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 343,
          "cds_start": 571,
          "cds_end": null,
          "cds_length": 1032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968713.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.568G>A",
          "hgvs_p": "p.Val190Met",
          "transcript": "ENST00000912585.1",
          "protein_id": "ENSP00000582644.1",
          "transcript_support_level": null,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 568,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912585.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.502G>A",
          "hgvs_p": "p.Val168Met",
          "transcript": "ENST00000912584.1",
          "protein_id": "ENSP00000582643.1",
          "transcript_support_level": null,
          "aa_start": 168,
          "aa_end": null,
          "aa_length": 320,
          "cds_start": 502,
          "cds_end": null,
          "cds_length": 963,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912584.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.499G>A",
          "hgvs_p": "p.Val167Met",
          "transcript": "NM_001349128.1",
          "protein_id": "NP_001336057.1",
          "transcript_support_level": null,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 319,
          "cds_start": 499,
          "cds_end": null,
          "cds_length": 960,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349128.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.499G>A",
          "hgvs_p": "p.Val167Met",
          "transcript": "ENST00000857683.1",
          "protein_id": "ENSP00000527742.1",
          "transcript_support_level": null,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 319,
          "cds_start": 499,
          "cds_end": null,
          "cds_length": 960,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857683.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.463G>A",
          "hgvs_p": "p.Val155Met",
          "transcript": "NM_001349127.2",
          "protein_id": "NP_001336056.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 312,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 939,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349127.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.475G>A",
          "hgvs_p": "p.Val159Met",
          "transcript": "ENST00000857689.1",
          "protein_id": "ENSP00000527748.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 311,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 936,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857689.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.472G>A",
          "hgvs_p": "p.Val158Met",
          "transcript": "ENST00000857688.1",
          "protein_id": "ENSP00000527747.1",
          "transcript_support_level": null,
          "aa_start": 158,
          "aa_end": null,
          "aa_length": 310,
          "cds_start": 472,
          "cds_end": null,
          "cds_length": 933,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857688.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.463G>A",
          "hgvs_p": "p.Val155Met",
          "transcript": "ENST00000857684.1",
          "protein_id": "ENSP00000527743.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857684.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.463G>A",
          "hgvs_p": "p.Val155Met",
          "transcript": "ENST00000968711.1",
          "protein_id": "ENSP00000638770.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968711.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.460G>A",
          "hgvs_p": "p.Val154Met",
          "transcript": "ENST00000857675.1",
          "protein_id": "ENSP00000527734.1",
          "transcript_support_level": null,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 306,
          "cds_start": 460,
          "cds_end": null,
          "cds_length": 921,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857675.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.460G>A",
          "hgvs_p": "p.Val154Met",
          "transcript": "ENST00000857677.1",
          "protein_id": "ENSP00000527736.1",
          "transcript_support_level": null,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 306,
          "cds_start": 460,
          "cds_end": null,
          "cds_length": 921,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857677.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.460G>A",
          "hgvs_p": "p.Val154Met",
          "transcript": "ENST00000857679.1",
          "protein_id": "ENSP00000527738.1",
          "transcript_support_level": null,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 306,
          "cds_start": 460,
          "cds_end": null,
          "cds_length": 921,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857679.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.391G>A",
          "hgvs_p": "p.Val131Met",
          "transcript": "ENST00000857680.1",
          "protein_id": "ENSP00000527739.1",
          "transcript_support_level": null,
          "aa_start": 131,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 391,
          "cds_end": null,
          "cds_length": 852,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857680.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CCDC24",
          "gene_hgnc_id": 28688,
          "hgvs_c": "c.391G>A",
          "hgvs_p": "p.Val131Met",
          "transcript": "ENST00000912586.1",
          "protein_id": "ENSP00000582645.1",
          "transcript_support_level": null,
          "aa_start": 131,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 391,
          "cds_end": null,
          "cds_length": 852,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912586.1"
        },
        {
          "aa_ref": "V",
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        {
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          "biotype": "nonsense_mediated_decay",
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      ],
      "gene_symbol": "CCDC24",
      "gene_hgnc_id": 28688,
      "dbsnp": "rs371107778",
      "frequency_reference_population": 0.000045843084,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 74,
      "gnomad_exomes_af": 0.0000451478,
      "gnomad_genomes_af": 0.0000525155,
      "gnomad_exomes_ac": 66,
      "gnomad_genomes_ac": 8,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.02116572856903076,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.052,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0976,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.54,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.256,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001349128.1",
          "gene_symbol": "CCDC24",
          "hgnc_id": 28688,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.499G>A",
          "hgvs_p": "p.Val167Met"
        },
        {
          "score": -5,
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          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BS1_Supporting"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000372306.7",
          "gene_symbol": "SLC6A9",
          "hgnc_id": 11056,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.1625-1938C>T",
          "hgvs_p": null
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}