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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-48298889-A-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=48298889&ref=A&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 48298889,
      "ref": "A",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_019073.4",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1291T>A",
          "hgvs_p": "p.Cys431Ser",
          "transcript": "NM_019073.4",
          "protein_id": "NP_061946.1",
          "transcript_support_level": null,
          "aa_start": 431,
          "aa_end": null,
          "aa_length": 488,
          "cds_start": 1291,
          "cds_end": null,
          "cds_length": 1467,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000371847.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_019073.4"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1291T>A",
          "hgvs_p": "p.Cys431Ser",
          "transcript": "ENST00000371847.8",
          "protein_id": "ENSP00000360913.3",
          "transcript_support_level": 1,
          "aa_start": 431,
          "aa_end": null,
          "aa_length": 488,
          "cds_start": 1291,
          "cds_end": null,
          "cds_length": 1467,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_019073.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371847.8"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1243T>A",
          "hgvs_p": "p.Cys415Ser",
          "transcript": "ENST00000371843.7",
          "protein_id": "ENSP00000360909.3",
          "transcript_support_level": 1,
          "aa_start": 415,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 1243,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371843.7"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1249T>A",
          "hgvs_p": "p.Cys417Ser",
          "transcript": "NM_001286239.2",
          "protein_id": "NP_001273168.1",
          "transcript_support_level": null,
          "aa_start": 417,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 1249,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286239.2"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1249T>A",
          "hgvs_p": "p.Cys417Ser",
          "transcript": "ENST00000396199.7",
          "protein_id": "ENSP00000379502.4",
          "transcript_support_level": 2,
          "aa_start": 417,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 1249,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396199.7"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1243T>A",
          "hgvs_p": "p.Cys415Ser",
          "transcript": "NM_001286238.2",
          "protein_id": "NP_001273167.1",
          "transcript_support_level": null,
          "aa_start": 415,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 1243,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286238.2"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1210T>A",
          "hgvs_p": "p.Cys404Ser",
          "transcript": "ENST00000872886.1",
          "protein_id": "ENSP00000542945.1",
          "transcript_support_level": null,
          "aa_start": 404,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": 1210,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872886.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1201T>A",
          "hgvs_p": "p.Cys401Ser",
          "transcript": "ENST00000872888.1",
          "protein_id": "ENSP00000542947.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 1201,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872888.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1162T>A",
          "hgvs_p": "p.Cys388Ser",
          "transcript": "ENST00000872885.1",
          "protein_id": "ENSP00000542944.1",
          "transcript_support_level": null,
          "aa_start": 388,
          "aa_end": null,
          "aa_length": 445,
          "cds_start": 1162,
          "cds_end": null,
          "cds_length": 1338,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872885.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1075T>A",
          "hgvs_p": "p.Cys359Ser",
          "transcript": "ENST00000930128.1",
          "protein_id": "ENSP00000600187.1",
          "transcript_support_level": null,
          "aa_start": 359,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 1075,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930128.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1006T>A",
          "hgvs_p": "p.Cys336Ser",
          "transcript": "ENST00000872887.1",
          "protein_id": "ENSP00000542946.1",
          "transcript_support_level": null,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 393,
          "cds_start": 1006,
          "cds_end": null,
          "cds_length": 1182,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872887.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.790T>A",
          "hgvs_p": "p.Cys264Ser",
          "transcript": "ENST00000930129.1",
          "protein_id": "ENSP00000600188.1",
          "transcript_support_level": null,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 790,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930129.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.502T>A",
          "hgvs_p": "p.Cys168Ser",
          "transcript": "ENST00000930127.1",
          "protein_id": "ENSP00000600186.1",
          "transcript_support_level": null,
          "aa_start": 168,
          "aa_end": null,
          "aa_length": 225,
          "cds_start": 502,
          "cds_end": null,
          "cds_length": 678,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930127.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1075T>A",
          "hgvs_p": "p.Cys359Ser",
          "transcript": "XM_017001510.2",
          "protein_id": "XP_016856999.1",
          "transcript_support_level": null,
          "aa_start": 359,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 1075,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017001510.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1199T>A",
          "hgvs_p": "p.Met400Lys",
          "transcript": "XM_017001511.3",
          "protein_id": "XP_016857000.1",
          "transcript_support_level": null,
          "aa_start": 400,
          "aa_end": null,
          "aa_length": 410,
          "cds_start": 1199,
          "cds_end": null,
          "cds_length": 1233,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017001511.3"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1027T>A",
          "hgvs_p": "p.Cys343Ser",
          "transcript": "XM_047422902.1",
          "protein_id": "XP_047278858.1",
          "transcript_support_level": null,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 1027,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047422902.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.1006T>A",
          "hgvs_p": "p.Cys336Ser",
          "transcript": "XM_011541606.3",
          "protein_id": "XP_011539908.1",
          "transcript_support_level": null,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 393,
          "cds_start": 1006,
          "cds_end": null,
          "cds_length": 1182,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011541606.3"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.979T>A",
          "hgvs_p": "p.Cys327Ser",
          "transcript": "XM_011541607.3",
          "protein_id": "XP_011539909.1",
          "transcript_support_level": null,
          "aa_start": 327,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 979,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011541607.3"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.967T>A",
          "hgvs_p": "p.Cys323Ser",
          "transcript": "XM_011541609.3",
          "protein_id": "XP_011539911.1",
          "transcript_support_level": null,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 380,
          "cds_start": 967,
          "cds_end": null,
          "cds_length": 1143,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011541609.3"
        },
        {
          "aa_ref": "C",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPATA6",
          "gene_hgnc_id": 18309,
          "hgvs_c": "c.967T>A",
          "hgvs_p": "p.Cys323Ser",
          "transcript": "XM_017001513.2",
          "protein_id": "XP_016857002.1",
          "transcript_support_level": null,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 380,
          "cds_start": 967,
          "cds_end": null,
          "cds_length": 1143,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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        {
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        {
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000603831.5"
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      ],
      "gene_symbol": "SPATA6",
      "gene_hgnc_id": 18309,
      "dbsnp": "rs748620425",
      "frequency_reference_population": 0.000002054392,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205439,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.018747806549072266,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.036,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0874,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.63,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.634,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_019073.4",
          "gene_symbol": "SPATA6",
          "hgnc_id": 18309,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1291T>A",
          "hgvs_p": "p.Cys431Ser"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}