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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-5877257-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=5877257&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 5877257,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_015102.5",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2653A>C",
          "hgvs_p": "p.Ser885Arg",
          "transcript": "NM_015102.5",
          "protein_id": "NP_055917.1",
          "transcript_support_level": null,
          "aa_start": 885,
          "aa_end": null,
          "aa_length": 1426,
          "cds_start": 2653,
          "cds_end": null,
          "cds_length": 4281,
          "cdna_start": 2873,
          "cdna_end": null,
          "cdna_length": 4955,
          "mane_select": "ENST00000378156.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015102.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2653A>C",
          "hgvs_p": "p.Ser885Arg",
          "transcript": "ENST00000378156.9",
          "protein_id": "ENSP00000367398.4",
          "transcript_support_level": 1,
          "aa_start": 885,
          "aa_end": null,
          "aa_length": 1426,
          "cds_start": 2653,
          "cds_end": null,
          "cds_length": 4281,
          "cdna_start": 2873,
          "cdna_end": null,
          "cdna_length": 4955,
          "mane_select": "NM_015102.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378156.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "n.*1554A>C",
          "hgvs_p": null,
          "transcript": "ENST00000378169.7",
          "protein_id": "ENSP00000367411.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4213,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000378169.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "n.*201A>C",
          "hgvs_p": null,
          "transcript": "ENST00000489180.6",
          "protein_id": "ENSP00000423747.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5548,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000489180.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "n.*1554A>C",
          "hgvs_p": null,
          "transcript": "ENST00000378169.7",
          "protein_id": "ENSP00000367411.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4213,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000378169.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "n.*201A>C",
          "hgvs_p": null,
          "transcript": "ENST00000489180.6",
          "protein_id": "ENSP00000423747.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5548,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000489180.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2653A>C",
          "hgvs_p": "p.Ser885Arg",
          "transcript": "ENST00000923561.1",
          "protein_id": "ENSP00000593620.1",
          "transcript_support_level": null,
          "aa_start": 885,
          "aa_end": null,
          "aa_length": 1426,
          "cds_start": 2653,
          "cds_end": null,
          "cds_length": 4281,
          "cdna_start": 2785,
          "cdna_end": null,
          "cdna_length": 4865,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923561.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2653A>C",
          "hgvs_p": "p.Ser885Arg",
          "transcript": "ENST00000923565.1",
          "protein_id": "ENSP00000593624.1",
          "transcript_support_level": null,
          "aa_start": 885,
          "aa_end": null,
          "aa_length": 1426,
          "cds_start": 2653,
          "cds_end": null,
          "cds_length": 4281,
          "cdna_start": 2861,
          "cdna_end": null,
          "cdna_length": 4937,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923565.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2650A>C",
          "hgvs_p": "p.Ser884Arg",
          "transcript": "ENST00000892397.1",
          "protein_id": "ENSP00000562456.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1425,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 4278,
          "cdna_start": 2889,
          "cdna_end": null,
          "cdna_length": 4971,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892397.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2650A>C",
          "hgvs_p": "p.Ser884Arg",
          "transcript": "ENST00000892402.1",
          "protein_id": "ENSP00000562461.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1425,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 4278,
          "cdna_start": 2987,
          "cdna_end": null,
          "cdna_length": 5069,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892402.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2650A>C",
          "hgvs_p": "p.Ser884Arg",
          "transcript": "ENST00000923562.1",
          "protein_id": "ENSP00000593621.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1425,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 4278,
          "cdna_start": 2775,
          "cdna_end": null,
          "cdna_length": 4855,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923562.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2650A>C",
          "hgvs_p": "p.Ser884Arg",
          "transcript": "ENST00000923564.1",
          "protein_id": "ENSP00000593623.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1425,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 4278,
          "cdna_start": 2890,
          "cdna_end": null,
          "cdna_length": 4972,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923564.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2650A>C",
          "hgvs_p": "p.Ser884Arg",
          "transcript": "ENST00000955275.1",
          "protein_id": "ENSP00000625334.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1425,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 4278,
          "cdna_start": 3570,
          "cdna_end": null,
          "cdna_length": 5645,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955275.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2653A>C",
          "hgvs_p": "p.Ser885Arg",
          "transcript": "ENST00000955267.1",
          "protein_id": "ENSP00000625326.1",
          "transcript_support_level": null,
          "aa_start": 885,
          "aa_end": null,
          "aa_length": 1423,
          "cds_start": 2653,
          "cds_end": null,
          "cds_length": 4272,
          "cdna_start": 2900,
          "cdna_end": null,
          "cdna_length": 4972,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955267.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2650A>C",
          "hgvs_p": "p.Ser884Arg",
          "transcript": "ENST00000955271.1",
          "protein_id": "ENSP00000625330.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1422,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 4269,
          "cdna_start": 2869,
          "cdna_end": null,
          "cdna_length": 4929,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955271.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2638A>C",
          "hgvs_p": "p.Ser880Arg",
          "transcript": "ENST00000955273.1",
          "protein_id": "ENSP00000625332.1",
          "transcript_support_level": null,
          "aa_start": 880,
          "aa_end": null,
          "aa_length": 1421,
          "cds_start": 2638,
          "cds_end": null,
          "cds_length": 4266,
          "cdna_start": 2885,
          "cdna_end": null,
          "cdna_length": 4919,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955273.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2653A>C",
          "hgvs_p": "p.Ser885Arg",
          "transcript": "ENST00000955266.1",
          "protein_id": "ENSP00000625325.1",
          "transcript_support_level": null,
          "aa_start": 885,
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          "aa_length": 1412,
          "cds_start": 2653,
          "cds_end": null,
          "cds_length": 4239,
          "cdna_start": 2939,
          "cdna_end": null,
          "cdna_length": 4977,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955266.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2650A>C",
          "hgvs_p": "p.Ser884Arg",
          "transcript": "ENST00000892399.1",
          "protein_id": "ENSP00000562458.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1411,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 4236,
          "cdna_start": 2872,
          "cdna_end": null,
          "cdna_length": 4912,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000892399.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2650A>C",
          "hgvs_p": "p.Ser884Arg",
          "transcript": "ENST00000955269.1",
          "protein_id": "ENSP00000625328.1",
          "transcript_support_level": null,
          "aa_start": 884,
          "aa_end": null,
          "aa_length": 1411,
          "cds_start": 2650,
          "cds_end": null,
          "cds_length": 4236,
          "cdna_start": 2859,
          "cdna_end": null,
          "cdna_length": 4897,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955269.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "c.2545A>C",
          "hgvs_p": "p.Ser849Arg",
          "transcript": "ENST00000892395.1",
          "protein_id": "ENSP00000562454.1",
          "transcript_support_level": null,
          "aa_start": 849,
          "aa_end": null,
          "aa_length": 1390,
          "cds_start": 2545,
          "cds_end": null,
          "cds_length": 4173,
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          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NPHP4",
          "gene_hgnc_id": 19104,
          "hgvs_c": "n.2691A>C",
          "hgvs_p": null,
          "transcript": "XR_007058632.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3200,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007058632.1"
        }
      ],
      "gene_symbol": "NPHP4",
      "gene_hgnc_id": 19104,
      "dbsnp": "rs112206586",
      "frequency_reference_population": 0.00016933389,
      "hom_count_reference_population": 2,
      "allele_count_reference_population": 272,
      "gnomad_exomes_af": 0.000085287,
      "gnomad_genomes_af": 0.000971256,
      "gnomad_exomes_ac": 124,
      "gnomad_genomes_ac": 148,
      "gnomad_exomes_homalt": 1,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0072517991065979,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.103,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.3111,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.36,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.385,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BS1_Supporting",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BS1_Supporting"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_015102.5",
          "gene_symbol": "NPHP4",
          "hgnc_id": 19104,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2653A>C",
          "hgvs_p": "p.Ser885Arg"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases,NPHP4-related disorder,Nephronophthisis,Nephronophthisis 4,Senior-Loken syndrome 4,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:4 LB:1",
      "phenotype_combined": "not provided|Nephronophthisis 4;Senior-Loken syndrome 4|Nephronophthisis|NPHP4-related disorder|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}
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