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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-61700997-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=61700997&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 61700997,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_032027.3",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr",
          "transcript": "NM_032027.3",
          "protein_id": "NP_114416.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 207,
          "cds_start": 376,
          "cds_end": null,
          "cds_length": 624,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000606498.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032027.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr",
          "transcript": "ENST00000606498.5",
          "protein_id": "ENSP00000475700.1",
          "transcript_support_level": 5,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 207,
          "cds_start": 376,
          "cds_end": null,
          "cds_length": 624,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_032027.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000606498.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr",
          "transcript": "ENST00000371180.7",
          "protein_id": "ENSP00000360222.2",
          "transcript_support_level": 5,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 207,
          "cds_start": 376,
          "cds_end": null,
          "cds_length": 624,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371180.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr",
          "transcript": "ENST00000371177.2",
          "protein_id": "ENSP00000475297.1",
          "transcript_support_level": 5,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 186,
          "cds_start": 376,
          "cds_end": null,
          "cds_length": 561,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371177.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.559G>A",
          "hgvs_p": "p.Ala187Thr",
          "transcript": "ENST00000964149.1",
          "protein_id": "ENSP00000634208.1",
          "transcript_support_level": null,
          "aa_start": 187,
          "aa_end": null,
          "aa_length": 268,
          "cds_start": 559,
          "cds_end": null,
          "cds_length": 807,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964149.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.541G>A",
          "hgvs_p": "p.Ala181Thr",
          "transcript": "ENST00000855760.1",
          "protein_id": "ENSP00000525819.1",
          "transcript_support_level": null,
          "aa_start": 181,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 541,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855760.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.481G>A",
          "hgvs_p": "p.Ala161Thr",
          "transcript": "ENST00000922032.1",
          "protein_id": "ENSP00000592091.1",
          "transcript_support_level": null,
          "aa_start": 161,
          "aa_end": null,
          "aa_length": 242,
          "cds_start": 481,
          "cds_end": null,
          "cds_length": 729,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922032.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr",
          "transcript": "ENST00000855758.1",
          "protein_id": "ENSP00000525817.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 207,
          "cds_start": 376,
          "cds_end": null,
          "cds_length": 624,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855758.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr",
          "transcript": "ENST00000922031.1",
          "protein_id": "ENSP00000592090.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 207,
          "cds_start": 376,
          "cds_end": null,
          "cds_length": 624,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922031.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr",
          "transcript": "ENST00000964150.1",
          "protein_id": "ENSP00000634209.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 207,
          "cds_start": 376,
          "cds_end": null,
          "cds_length": 624,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964150.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr",
          "transcript": "ENST00000964148.1",
          "protein_id": "ENSP00000634207.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 205,
          "cds_start": 376,
          "cds_end": null,
          "cds_length": 618,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964148.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.361G>A",
          "hgvs_p": "p.Ala121Thr",
          "transcript": "ENST00000855759.1",
          "protein_id": "ENSP00000525818.1",
          "transcript_support_level": null,
          "aa_start": 121,
          "aa_end": null,
          "aa_length": 202,
          "cds_start": 361,
          "cds_end": null,
          "cds_length": 609,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855759.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.541G>A",
          "hgvs_p": "p.Ala181Thr",
          "transcript": "XM_047431743.1",
          "protein_id": "XP_047287699.1",
          "transcript_support_level": null,
          "aa_start": 181,
          "aa_end": null,
          "aa_length": 277,
          "cds_start": 541,
          "cds_end": null,
          "cds_length": 834,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047431743.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.541G>A",
          "hgvs_p": "p.Ala181Thr",
          "transcript": "XM_047431744.1",
          "protein_id": "XP_047287700.1",
          "transcript_support_level": null,
          "aa_start": 181,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 541,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047431744.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.541G>A",
          "hgvs_p": "p.Ala181Thr",
          "transcript": "XM_047431745.1",
          "protein_id": "XP_047287701.1",
          "transcript_support_level": null,
          "aa_start": 181,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 541,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047431745.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.239-6227G>A",
          "hgvs_p": null,
          "transcript": "ENST00000922030.1",
          "protein_id": "ENSP00000592089.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 140,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 423,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922030.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "c.220-17451G>A",
          "hgvs_p": null,
          "transcript": "ENST00000855761.1",
          "protein_id": "ENSP00000525820.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 109,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 330,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855761.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "n.376G>A",
          "hgvs_p": null,
          "transcript": "ENST00000371178.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000371178.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "n.*186G>A",
          "hgvs_p": null,
          "transcript": "ENST00000468586.5",
          "protein_id": "ENSP00000475186.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000468586.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TM2D1",
          "gene_hgnc_id": 24142,
          "hgvs_c": "n.119G>A",
          "hgvs_p": null,
          "transcript": "ENST00000472989.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000472989.5"
        },
        {
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      ],
      "gene_symbol": "TM2D1",
      "gene_hgnc_id": 24142,
      "dbsnp": "rs778917284",
      "frequency_reference_population": 0.000007553482,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 11,
      "gnomad_exomes_af": 0.00000755348,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 11,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6775627732276917,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.10999999940395355,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.2,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.5695,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.09,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.081,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.11,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_032027.3",
          "gene_symbol": "TM2D1",
          "hgnc_id": 24142,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.376G>A",
          "hgvs_p": "p.Ala126Thr"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}