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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-63532754-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=63532754&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 63532754,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_032437.4",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.484A>C",
          "hgvs_p": "p.Lys162Gln",
          "transcript": "NM_032437.4",
          "protein_id": "NP_115813.2",
          "transcript_support_level": null,
          "aa_start": 162,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 484,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000371088.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032437.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.484A>C",
          "hgvs_p": "p.Lys162Gln",
          "transcript": "ENST00000371088.5",
          "protein_id": "ENSP00000360129.4",
          "transcript_support_level": 1,
          "aa_start": 162,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 484,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_032437.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371088.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.484A>C",
          "hgvs_p": "p.Lys162Gln",
          "transcript": "ENST00000927137.1",
          "protein_id": "ENSP00000597196.1",
          "transcript_support_level": null,
          "aa_start": 162,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 484,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000927137.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.484A>C",
          "hgvs_p": "p.Lys162Gln",
          "transcript": "ENST00000971339.1",
          "protein_id": "ENSP00000641398.1",
          "transcript_support_level": null,
          "aa_start": 162,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 484,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971339.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.484A>C",
          "hgvs_p": "p.Lys162Gln",
          "transcript": "ENST00000971337.1",
          "protein_id": "ENSP00000641396.1",
          "transcript_support_level": null,
          "aa_start": 162,
          "aa_end": null,
          "aa_length": 619,
          "cds_start": 484,
          "cds_end": null,
          "cds_length": 1860,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971337.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.484A>C",
          "hgvs_p": "p.Lys162Gln",
          "transcript": "ENST00000971338.1",
          "protein_id": "ENSP00000641397.1",
          "transcript_support_level": null,
          "aa_start": 162,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 484,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971338.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.523A>C",
          "hgvs_p": "p.Lys175Gln",
          "transcript": "XM_006710976.4",
          "protein_id": "XP_006711039.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 642,
          "cds_start": 523,
          "cds_end": null,
          "cds_length": 1929,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006710976.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.484A>C",
          "hgvs_p": "p.Lys162Gln",
          "transcript": "XM_006710977.2",
          "protein_id": "XP_006711040.1",
          "transcript_support_level": null,
          "aa_start": 162,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 484,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006710977.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.523A>C",
          "hgvs_p": "p.Lys175Gln",
          "transcript": "XM_011542301.3",
          "protein_id": "XP_011540603.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 619,
          "cds_start": 523,
          "cds_end": null,
          "cds_length": 1860,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011542301.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "c.523A>C",
          "hgvs_p": "p.Lys175Gln",
          "transcript": "XM_047432129.1",
          "protein_id": "XP_047288085.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 329,
          "cds_start": 523,
          "cds_end": null,
          "cds_length": 990,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047432129.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "n.530A>C",
          "hgvs_p": null,
          "transcript": "XR_946779.4",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_946779.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ITGB3BP",
          "gene_hgnc_id": 6157,
          "hgvs_c": "n.198-3568T>G",
          "hgvs_p": null,
          "transcript": "ENST00000478138.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000478138.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ITGB3BP",
          "gene_hgnc_id": 6157,
          "hgvs_c": "n.105-3568T>G",
          "hgvs_p": null,
          "transcript": "ENST00000717656.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000717656.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFCAB7",
          "gene_hgnc_id": 29379,
          "hgvs_c": "n.*58A>C",
          "hgvs_p": null,
          "transcript": "ENST00000480886.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000480886.1"
        }
      ],
      "gene_symbol": "EFCAB7",
      "gene_hgnc_id": 29379,
      "dbsnp": "rs151059690",
      "frequency_reference_population": 0.0000020761331,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000207613,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5200216174125671,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.5519999861717224,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.291,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1362,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.24,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.616,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.422624362707824,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_032437.4",
          "gene_symbol": "EFCAB7",
          "hgnc_id": 29379,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.484A>C",
          "hgvs_p": "p.Lys162Gln"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000478138.1",
          "gene_symbol": "ITGB3BP",
          "hgnc_id": 6157,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "n.198-3568T>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}