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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-77697904-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=77697904&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 77697904,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_015017.5",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2537A>G",
          "hgvs_p": "p.Lys846Arg",
          "transcript": "NM_201624.3",
          "protein_id": "NP_963918.1",
          "transcript_support_level": null,
          "aa_start": 846,
          "aa_end": null,
          "aa_length": 911,
          "cds_start": 2537,
          "cds_end": null,
          "cds_length": 2736,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000370794.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_201624.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2537A>G",
          "hgvs_p": "p.Lys846Arg",
          "transcript": "ENST00000370794.7",
          "protein_id": "ENSP00000359830.3",
          "transcript_support_level": 1,
          "aa_start": 846,
          "aa_end": null,
          "aa_length": 911,
          "cds_start": 2537,
          "cds_end": null,
          "cds_length": 2736,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_201624.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000370794.7"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2630A>G",
          "hgvs_p": "p.Lys877Arg",
          "transcript": "ENST00000370793.5",
          "protein_id": "ENSP00000359829.1",
          "transcript_support_level": 1,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 2630,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000370793.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2630A>G",
          "hgvs_p": "p.Lys877Arg",
          "transcript": "NM_015017.5",
          "protein_id": "NP_055832.3",
          "transcript_support_level": null,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 2630,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015017.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2630A>G",
          "hgvs_p": "p.Lys877Arg",
          "transcript": "ENST00000357428.5",
          "protein_id": "ENSP00000350009.1",
          "transcript_support_level": 5,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 2630,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000357428.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2606A>G",
          "hgvs_p": "p.Lys869Arg",
          "transcript": "NM_001377430.1",
          "protein_id": "NP_001364359.1",
          "transcript_support_level": null,
          "aa_start": 869,
          "aa_end": null,
          "aa_length": 934,
          "cds_start": 2606,
          "cds_end": null,
          "cds_length": 2805,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377430.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2534A>G",
          "hgvs_p": "p.Lys845Arg",
          "transcript": "ENST00000920219.1",
          "protein_id": "ENSP00000590278.1",
          "transcript_support_level": null,
          "aa_start": 845,
          "aa_end": null,
          "aa_length": 910,
          "cds_start": 2534,
          "cds_end": null,
          "cds_length": 2733,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920219.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2537A>G",
          "hgvs_p": "p.Lys846Arg",
          "transcript": "ENST00000955732.1",
          "protein_id": "ENSP00000625791.1",
          "transcript_support_level": null,
          "aa_start": 846,
          "aa_end": null,
          "aa_length": 910,
          "cds_start": 2537,
          "cds_end": null,
          "cds_length": 2733,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955732.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2537A>G",
          "hgvs_p": "p.Lys846Arg",
          "transcript": "ENST00000853335.1",
          "protein_id": "ENSP00000523394.1",
          "transcript_support_level": null,
          "aa_start": 846,
          "aa_end": null,
          "aa_length": 909,
          "cds_start": 2537,
          "cds_end": null,
          "cds_length": 2730,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853335.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2513A>G",
          "hgvs_p": "p.Lys838Arg",
          "transcript": "NM_001377431.1",
          "protein_id": "NP_001364360.1",
          "transcript_support_level": null,
          "aa_start": 838,
          "aa_end": null,
          "aa_length": 903,
          "cds_start": 2513,
          "cds_end": null,
          "cds_length": 2712,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377431.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2513A>G",
          "hgvs_p": "p.Lys838Arg",
          "transcript": "ENST00000853331.1",
          "protein_id": "ENSP00000523390.1",
          "transcript_support_level": null,
          "aa_start": 838,
          "aa_end": null,
          "aa_length": 903,
          "cds_start": 2513,
          "cds_end": null,
          "cds_length": 2712,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853331.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2513A>G",
          "hgvs_p": "p.Lys838Arg",
          "transcript": "ENST00000853332.1",
          "protein_id": "ENSP00000523391.1",
          "transcript_support_level": null,
          "aa_start": 838,
          "aa_end": null,
          "aa_length": 903,
          "cds_start": 2513,
          "cds_end": null,
          "cds_length": 2712,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853332.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2513A>G",
          "hgvs_p": "p.Lys838Arg",
          "transcript": "ENST00000920221.1",
          "protein_id": "ENSP00000590280.1",
          "transcript_support_level": null,
          "aa_start": 838,
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          "aa_length": 903,
          "cds_start": 2513,
          "cds_end": null,
          "cds_length": 2712,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000920221.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2513A>G",
          "hgvs_p": "p.Lys838Arg",
          "transcript": "ENST00000920223.1",
          "protein_id": "ENSP00000590282.1",
          "transcript_support_level": null,
          "aa_start": 838,
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          "aa_length": 901,
          "cds_start": 2513,
          "cds_end": null,
          "cds_length": 2706,
          "cdna_start": null,
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          "mane_select": null,
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        },
        {
          "aa_ref": "K",
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          ],
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2498A>G",
          "hgvs_p": "p.Lys833Arg",
          "transcript": "ENST00000955725.1",
          "protein_id": "ENSP00000625784.1",
          "transcript_support_level": null,
          "aa_start": 833,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": 2498,
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          "cds_length": 2697,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000955725.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2495A>G",
          "hgvs_p": "p.Lys832Arg",
          "transcript": "ENST00000955726.1",
          "protein_id": "ENSP00000625785.1",
          "transcript_support_level": null,
          "aa_start": 832,
          "aa_end": null,
          "aa_length": 897,
          "cds_start": 2495,
          "cds_end": null,
          "cds_length": 2694,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000955726.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2384A>G",
          "hgvs_p": "p.Lys795Arg",
          "transcript": "ENST00000853330.1",
          "protein_id": "ENSP00000523389.1",
          "transcript_support_level": null,
          "aa_start": 795,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "K",
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          ],
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          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
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          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
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          "hgvs_p": "p.Lys795Arg",
          "transcript": "ENST00000955734.1",
          "protein_id": "ENSP00000625793.1",
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          "biotype": "protein_coding",
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        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2360A>G",
          "hgvs_p": "p.Lys787Arg",
          "transcript": "ENST00000920222.1",
          "protein_id": "ENSP00000590281.1",
          "transcript_support_level": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000920222.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2360A>G",
          "hgvs_p": "p.Lys787Arg",
          "transcript": "ENST00000955730.1",
          "protein_id": "ENSP00000625789.1",
          "transcript_support_level": null,
          "aa_start": 787,
          "aa_end": null,
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          "cdna_start": null,
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      "splice_source_selected": "max_spliceai",
      "revel_score": 0.159,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0777,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.38,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.583,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_015017.5",
          "gene_symbol": "USP33",
          "hgnc_id": 20059,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.2630A>G",
          "hgvs_p": "p.Lys877Arg"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}