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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-77714760-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=77714760&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 77714760,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_015017.5",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2069A>G",
          "hgvs_p": "p.Lys690Arg",
          "transcript": "NM_201624.3",
          "protein_id": "NP_963918.1",
          "transcript_support_level": null,
          "aa_start": 690,
          "aa_end": null,
          "aa_length": 911,
          "cds_start": 2069,
          "cds_end": null,
          "cds_length": 2736,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000370794.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_201624.3"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2069A>G",
          "hgvs_p": "p.Lys690Arg",
          "transcript": "ENST00000370794.7",
          "protein_id": "ENSP00000359830.3",
          "transcript_support_level": 1,
          "aa_start": 690,
          "aa_end": null,
          "aa_length": 911,
          "cds_start": 2069,
          "cds_end": null,
          "cds_length": 2736,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_201624.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000370794.7"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2162A>G",
          "hgvs_p": "p.Lys721Arg",
          "transcript": "ENST00000370793.5",
          "protein_id": "ENSP00000359829.1",
          "transcript_support_level": 1,
          "aa_start": 721,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 2162,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000370793.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2138A>G",
          "hgvs_p": "p.Lys713Arg",
          "transcript": "ENST00000370792.7",
          "protein_id": "ENSP00000359828.3",
          "transcript_support_level": 1,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 828,
          "cds_start": 2138,
          "cds_end": null,
          "cds_length": 2487,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000370792.7"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2162A>G",
          "hgvs_p": "p.Lys721Arg",
          "transcript": "NM_015017.5",
          "protein_id": "NP_055832.3",
          "transcript_support_level": null,
          "aa_start": 721,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 2162,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015017.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2162A>G",
          "hgvs_p": "p.Lys721Arg",
          "transcript": "ENST00000357428.5",
          "protein_id": "ENSP00000350009.1",
          "transcript_support_level": 5,
          "aa_start": 721,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 2162,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000357428.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2138A>G",
          "hgvs_p": "p.Lys713Arg",
          "transcript": "NM_001377430.1",
          "protein_id": "NP_001364359.1",
          "transcript_support_level": null,
          "aa_start": 713,
          "aa_end": null,
          "aa_length": 934,
          "cds_start": 2138,
          "cds_end": null,
          "cds_length": 2805,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377430.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2066A>G",
          "hgvs_p": "p.Lys689Arg",
          "transcript": "ENST00000920219.1",
          "protein_id": "ENSP00000590278.1",
          "transcript_support_level": null,
          "aa_start": 689,
          "aa_end": null,
          "aa_length": 910,
          "cds_start": 2066,
          "cds_end": null,
          "cds_length": 2733,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920219.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2069A>G",
          "hgvs_p": "p.Lys690Arg",
          "transcript": "ENST00000955732.1",
          "protein_id": "ENSP00000625791.1",
          "transcript_support_level": null,
          "aa_start": 690,
          "aa_end": null,
          "aa_length": 910,
          "cds_start": 2069,
          "cds_end": null,
          "cds_length": 2733,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955732.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2069A>G",
          "hgvs_p": "p.Lys690Arg",
          "transcript": "ENST00000853335.1",
          "protein_id": "ENSP00000523394.1",
          "transcript_support_level": null,
          "aa_start": 690,
          "aa_end": null,
          "aa_length": 909,
          "cds_start": 2069,
          "cds_end": null,
          "cds_length": 2730,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853335.1"
        },
        {
          "aa_ref": "K",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2045A>G",
          "hgvs_p": "p.Lys682Arg",
          "transcript": "NM_001377431.1",
          "protein_id": "NP_001364360.1",
          "transcript_support_level": null,
          "aa_start": 682,
          "aa_end": null,
          "aa_length": 903,
          "cds_start": 2045,
          "cds_end": null,
          "cds_length": 2712,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001377431.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2045A>G",
          "hgvs_p": "p.Lys682Arg",
          "transcript": "ENST00000853331.1",
          "protein_id": "ENSP00000523390.1",
          "transcript_support_level": null,
          "aa_start": 682,
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          "cds_start": 2045,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000853331.1"
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
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          "intron_rank": null,
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          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2045A>G",
          "hgvs_p": "p.Lys682Arg",
          "transcript": "ENST00000853332.1",
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000853332.1"
        },
        {
          "aa_ref": "K",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2045A>G",
          "hgvs_p": "p.Lys682Arg",
          "transcript": "ENST00000920221.1",
          "protein_id": "ENSP00000590280.1",
          "transcript_support_level": null,
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        {
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          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2045A>G",
          "hgvs_p": "p.Lys682Arg",
          "transcript": "ENST00000920223.1",
          "protein_id": "ENSP00000590282.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000920223.1"
        },
        {
          "aa_ref": "K",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 19,
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          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2030A>G",
          "hgvs_p": "p.Lys677Arg",
          "transcript": "ENST00000955725.1",
          "protein_id": "ENSP00000625784.1",
          "transcript_support_level": null,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": 2030,
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        },
        {
          "aa_ref": "K",
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          "strand": false,
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          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.2027A>G",
          "hgvs_p": "p.Lys676Arg",
          "transcript": "ENST00000955726.1",
          "protein_id": "ENSP00000625785.1",
          "transcript_support_level": null,
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        {
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          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
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        {
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          "gene_symbol": "USP33",
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          "biotype": "protein_coding",
          "feature": "ENST00000955734.1"
        },
        {
          "aa_ref": "K",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "c.1892A>G",
          "hgvs_p": "p.Lys631Arg",
          "transcript": "ENST00000920222.1",
          "protein_id": "ENSP00000590281.1",
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          "aa_length": 852,
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          "feature": "XM_047449735.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
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          "exon_count": 3,
          "intron_rank": null,
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          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "n.158A>G",
          "hgvs_p": null,
          "transcript": "ENST00000472462.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000472462.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "USP33",
          "gene_hgnc_id": 20059,
          "hgvs_c": "n.386+982A>G",
          "hgvs_p": null,
          "transcript": "ENST00000527390.1",
          "protein_id": "ENSP00000432667.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000527390.1"
        }
      ],
      "gene_symbol": "USP33",
      "gene_hgnc_id": 20059,
      "dbsnp": "rs144899790",
      "frequency_reference_population": 0.00061844493,
      "hom_count_reference_population": 2,
      "allele_count_reference_population": 997,
      "gnomad_exomes_af": 0.000656896,
      "gnomad_genomes_af": 0.000249652,
      "gnomad_exomes_ac": 959,
      "gnomad_genomes_ac": 38,
      "gnomad_exomes_homalt": 2,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.03999727964401245,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.108,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0709,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.61,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.977,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -8,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -8,
          "benign_score": 8,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_015017.5",
          "gene_symbol": "USP33",
          "hgnc_id": 20059,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.2162A>G",
          "hgvs_p": "p.Lys721Arg"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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