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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-78016018-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=78016018&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 78016018,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_007034.5",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "NM_007034.5",
          "protein_id": "NP_008965.2",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 957,
          "cdna_end": null,
          "cdna_length": 2903,
          "mane_select": "ENST00000370763.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_007034.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000370763.6",
          "protein_id": "ENSP00000359799.5",
          "transcript_support_level": 1,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 957,
          "cdna_end": null,
          "cdna_length": 2903,
          "mane_select": "NM_007034.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000370763.6"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "NM_001317099.2",
          "protein_id": "NP_001304028.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 955,
          "cdna_end": null,
          "cdna_length": 2901,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317099.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000867099.1",
          "protein_id": "ENSP00000537158.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 1040,
          "cdna_end": null,
          "cdna_length": 2986,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867099.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000867100.1",
          "protein_id": "ENSP00000537159.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 1145,
          "cdna_end": null,
          "cdna_length": 3091,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867100.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000939131.1",
          "protein_id": "ENSP00000609190.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 897,
          "cdna_end": null,
          "cdna_length": 2197,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939131.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000939132.1",
          "protein_id": "ENSP00000609191.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 1258,
          "cdna_end": null,
          "cdna_length": 2554,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939132.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000939133.1",
          "protein_id": "ENSP00000609192.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 1176,
          "cdna_end": null,
          "cdna_length": 2475,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939133.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000939134.1",
          "protein_id": "ENSP00000609193.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 1267,
          "cdna_end": null,
          "cdna_length": 2455,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939134.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000939135.1",
          "protein_id": "ENSP00000609194.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 1196,
          "cdna_end": null,
          "cdna_length": 2386,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939135.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000939136.1",
          "protein_id": "ENSP00000609195.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 2698,
          "cdna_end": null,
          "cdna_length": 3997,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939136.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser",
          "transcript": "ENST00000957220.1",
          "protein_id": "ENSP00000627279.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 1048,
          "cdna_end": null,
          "cdna_length": 3559,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957220.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.665T>C",
          "hgvs_p": "p.Leu222Ser",
          "transcript": "NM_001317100.2",
          "protein_id": "NP_001304029.1",
          "transcript_support_level": null,
          "aa_start": 222,
          "aa_end": null,
          "aa_length": 297,
          "cds_start": 665,
          "cds_end": null,
          "cds_length": 894,
          "cdna_start": 1132,
          "cdna_end": null,
          "cdna_length": 3078,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317100.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.440T>C",
          "hgvs_p": "p.Leu147Ser",
          "transcript": "NM_001317101.2",
          "protein_id": "NP_001304030.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 222,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 669,
          "cdna_start": 1019,
          "cdna_end": null,
          "cdna_length": 2965,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317101.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.440T>C",
          "hgvs_p": "p.Leu147Ser",
          "transcript": "NM_001317102.2",
          "protein_id": "NP_001304031.1",
          "transcript_support_level": null,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 222,
          "cds_start": 440,
          "cds_end": null,
          "cds_length": 669,
          "cdna_start": 693,
          "cdna_end": null,
          "cdna_length": 2639,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317102.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "c.216T>C",
          "hgvs_p": "p.Ile72Ile",
          "transcript": "NM_001317103.2",
          "protein_id": "NP_001304032.1",
          "transcript_support_level": null,
          "aa_start": 72,
          "aa_end": null,
          "aa_length": 77,
          "cds_start": 216,
          "cds_end": null,
          "cds_length": 234,
          "cdna_start": 388,
          "cdna_end": null,
          "cdna_length": 2334,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001317103.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "n.392T>C",
          "hgvs_p": null,
          "transcript": "ENST00000476396.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 673,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000476396.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAJB4",
          "gene_hgnc_id": 14886,
          "hgvs_c": "n.693T>C",
          "hgvs_p": null,
          "transcript": "ENST00000487931.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 949,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000487931.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "GIPC2",
          "gene_hgnc_id": 18177,
          "hgvs_c": "n.78+36399T>C",
          "hgvs_p": null,
          "transcript": "ENST00000476882.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 312,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000476882.1"
        }
      ],
      "gene_symbol": "DNAJB4",
      "gene_hgnc_id": 14886,
      "dbsnp": null,
      "frequency_reference_population": 6.8435025e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.8435e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9180591106414795,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.688,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9975,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.27,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.017,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP2,PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_007034.5",
          "gene_symbol": "DNAJB4",
          "hgnc_id": 14886,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.785T>C",
          "hgvs_p": "p.Leu262Ser"
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000476882.1",
          "gene_symbol": "GIPC2",
          "hgnc_id": 18177,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "n.78+36399T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Congenital myopathy 21 with early respiratory failure",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Congenital myopathy 21 with early respiratory failure",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}
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