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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-8863252-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=8863252&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 8863252,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001428.5",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "NM_001428.5",
          "protein_id": "NP_001419.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000234590.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001428.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000234590.10",
          "protein_id": "ENSP00000234590.4",
          "transcript_support_level": 1,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001428.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000234590.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "n.2044G>C",
          "hgvs_p": null,
          "transcript": "ENST00000464920.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000464920.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1189G>C",
          "hgvs_p": "p.Gly397Arg",
          "transcript": "ENST00000879697.1",
          "protein_id": "ENSP00000549756.1",
          "transcript_support_level": null,
          "aa_start": 397,
          "aa_end": null,
          "aa_length": 444,
          "cds_start": 1189,
          "cds_end": null,
          "cds_length": 1335,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879697.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1189G>C",
          "hgvs_p": "p.Gly397Arg",
          "transcript": "ENST00000935305.1",
          "protein_id": "ENSP00000605364.1",
          "transcript_support_level": null,
          "aa_start": 397,
          "aa_end": null,
          "aa_length": 442,
          "cds_start": 1189,
          "cds_end": null,
          "cds_length": 1329,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935305.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "NM_001353346.3",
          "protein_id": "NP_001340275.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353346.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000647408.1",
          "protein_id": "ENSP00000495530.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000647408.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879694.1",
          "protein_id": "ENSP00000549753.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879694.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879696.1",
          "protein_id": "ENSP00000549755.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879696.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879698.1",
          "protein_id": "ENSP00000549757.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879698.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879700.1",
          "protein_id": "ENSP00000549759.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879700.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879703.1",
          "protein_id": "ENSP00000549762.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879703.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879704.1",
          "protein_id": "ENSP00000549763.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879704.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879705.1",
          "protein_id": "ENSP00000549764.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879705.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879706.1",
          "protein_id": "ENSP00000549765.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879706.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000879707.1",
          "protein_id": "ENSP00000549766.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879707.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000935295.1",
          "protein_id": "ENSP00000605354.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935295.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000935306.1",
          "protein_id": "ENSP00000605365.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935306.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000935307.1",
          "protein_id": "ENSP00000605366.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935307.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENO1",
          "gene_hgnc_id": 3350,
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg",
          "transcript": "ENST00000971792.1",
          "protein_id": "ENSP00000641851.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 434,
          "cds_start": 1159,
          "cds_end": null,
          "cds_length": 1305,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971792.1"
        },
        {
          "aa_ref": "G",
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          "transcript": "ENST00000879702.1",
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          "cds_length": 1137,
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        {
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          "consequences": [
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          "transcript": "ENST00000645609.1",
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          "biotype": "retained_intron",
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        {
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          "protein_coding": false,
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          "consequences": [
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          "gene_symbol": "ENO1",
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          "hgvs_c": "n.*912G>C",
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          "transcript": "ENST00000646370.2",
          "protein_id": "ENSP00000495568.1",
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          "biotype": "nonsense_mediated_decay",
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        {
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          "protein_coding": false,
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          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 11,
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          "exon_count": 13,
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          "gene_symbol": "ENO1",
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          "hgvs_c": "n.*912G>C",
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          "transcript": "ENST00000646370.2",
          "protein_id": "ENSP00000495568.1",
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          "cdna_length": null,
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000646370.2"
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      ],
      "gene_symbol": "ENO1",
      "gene_hgnc_id": 3350,
      "dbsnp": "rs11544489",
      "frequency_reference_population": 6.84061e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84061e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9804259538650513,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.792,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.998,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.2,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.785,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001428.5",
          "gene_symbol": "ENO1",
          "hgnc_id": 3350,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.1159G>C",
          "hgvs_p": "p.Gly387Arg"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}