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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-99913653-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=99913653&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 99913653,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000361915.8",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "NM_000642.3",
          "protein_id": "NP_000633.2",
          "transcript_support_level": null,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4199,
          "cdna_end": null,
          "cdna_length": 7091,
          "mane_select": "ENST00000361915.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "ENST00000361915.8",
          "protein_id": "ENSP00000355106.3",
          "transcript_support_level": 1,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4199,
          "cdna_end": null,
          "cdna_length": 7091,
          "mane_select": "NM_000642.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "ENST00000294724.8",
          "protein_id": "ENSP00000294724.4",
          "transcript_support_level": 1,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4554,
          "cdna_end": null,
          "cdna_length": 7446,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "ENST00000370163.7",
          "protein_id": "ENSP00000359182.3",
          "transcript_support_level": 1,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4274,
          "cdna_end": null,
          "cdna_length": 7166,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "ENST00000370165.7",
          "protein_id": "ENSP00000359184.3",
          "transcript_support_level": 1,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4214,
          "cdna_end": null,
          "cdna_length": 7106,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "n.*4012G>A",
          "hgvs_p": null,
          "transcript": "ENST00000361302.7",
          "protein_id": "ENSP00000354971.4",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7179,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "n.4287G>A",
          "hgvs_p": null,
          "transcript": "ENST00000637337.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7179,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "n.*4012G>A",
          "hgvs_p": null,
          "transcript": "ENST00000361302.7",
          "protein_id": "ENSP00000354971.4",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7179,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "NM_000028.3",
          "protein_id": "NP_000019.2",
          "transcript_support_level": null,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4422,
          "cdna_end": null,
          "cdna_length": 7314,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "NM_000643.3",
          "protein_id": "NP_000634.2",
          "transcript_support_level": null,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4402,
          "cdna_end": null,
          "cdna_length": 7294,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "NM_000644.3",
          "protein_id": "NP_000635.2",
          "transcript_support_level": null,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4342,
          "cdna_end": null,
          "cdna_length": 7234,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "NM_001425325.1",
          "protein_id": "NP_001412254.1",
          "transcript_support_level": null,
          "aa_start": 1359,
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          "aa_length": 1532,
          "cds_start": 4076,
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          "cdna_start": 4139,
          "cdna_end": null,
          "cdna_length": 7031,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4055G>A",
          "hgvs_p": "p.Arg1352His",
          "transcript": "NM_001425326.1",
          "protein_id": "NP_001412255.1",
          "transcript_support_level": null,
          "aa_start": 1352,
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          "aa_length": 1525,
          "cds_start": 4055,
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          "cds_length": 4578,
          "cdna_start": 4178,
          "cdna_end": null,
          "cdna_length": 7070,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 31,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4028G>A",
          "hgvs_p": "p.Arg1343His",
          "transcript": "NM_000646.3",
          "protein_id": "NP_000637.2",
          "transcript_support_level": null,
          "aa_start": 1343,
          "aa_end": null,
          "aa_length": 1516,
          "cds_start": 4028,
          "cds_end": null,
          "cds_length": 4551,
          "cdna_start": 4342,
          "cdna_end": null,
          "cdna_length": 7234,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
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          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4028G>A",
          "hgvs_p": "p.Arg1343His",
          "transcript": "ENST00000370161.6",
          "protein_id": "ENSP00000359180.2",
          "transcript_support_level": 5,
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          "cdna_start": 4028,
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          "cdna_length": 6923,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.3875G>A",
          "hgvs_p": "p.Arg1292His",
          "transcript": "NM_001425327.1",
          "protein_id": "NP_001412256.1",
          "transcript_support_level": null,
          "aa_start": 1292,
          "aa_end": null,
          "aa_length": 1465,
          "cds_start": 3875,
          "cds_end": null,
          "cds_length": 4398,
          "cdna_start": 3998,
          "cdna_end": null,
          "cdna_length": 6890,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.3872G>A",
          "hgvs_p": "p.Arg1291His",
          "transcript": "NM_001425328.1",
          "protein_id": "NP_001412257.1",
          "transcript_support_level": null,
          "aa_start": 1291,
          "aa_end": null,
          "aa_length": 1464,
          "cds_start": 3872,
          "cds_end": null,
          "cds_length": 4395,
          "cdna_start": 3995,
          "cdna_end": null,
          "cdna_length": 6887,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
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          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.3737G>A",
          "hgvs_p": "p.Arg1246His",
          "transcript": "NM_001425329.1",
          "protein_id": "NP_001412258.1",
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          "cds_start": 3737,
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          "cds_length": 4260,
          "cdna_start": 3860,
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        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.3698G>A",
          "hgvs_p": "p.Arg1233His",
          "transcript": "NM_001425332.1",
          "protein_id": "NP_001412261.1",
          "transcript_support_level": null,
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          "aa_length": 1406,
          "cds_start": 3698,
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          "cdna_start": 3821,
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          "cdna_length": 6713,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
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          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His",
          "transcript": "XM_005270557.3",
          "protein_id": "XP_005270614.1",
          "transcript_support_level": null,
          "aa_start": 1359,
          "aa_end": null,
          "aa_length": 1532,
          "cds_start": 4076,
          "cds_end": null,
          "cds_length": 4599,
          "cdna_start": 4988,
          "cdna_end": null,
          "cdna_length": 7883,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGL",
          "gene_hgnc_id": 321,
          "hgvs_c": "c.2336G>A",
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      "dbsnp": "rs201533636",
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      "allele_count_reference_population": 379,
      "gnomad_exomes_af": 0.000245589,
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      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.8268964290618896,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.649,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.1627,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.04,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 5.138,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 0,
          "pathogenic_score": 1,
          "criteria": [
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          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000361915.8",
          "gene_symbol": "AGL",
          "hgnc_id": 321,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.4076G>A",
          "hgvs_p": "p.Arg1359His"
        },
        {
          "score": 1,
          "benign_score": 0,
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          "criteria": [
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000840838.1",
          "gene_symbol": "ENSG00000288826",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.488-436C>T",
          "hgvs_p": null
        },
        {
          "score": 1,
          "benign_score": 0,
          "pathogenic_score": 1,
          "criteria": [
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_007066243.1",
          "gene_symbol": "LOC124904230",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.671-436C>T",
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        }
      ],
      "clinvar_disease": "Glycogen storage disease type III,Inborn genetic diseases,Meniere disease,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:6",
      "phenotype_combined": "Glycogen storage disease type III|not provided|Meniere disease|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}