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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-13132107-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=13132107&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 13132107,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_021980.4",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "NM_001008212.2",
          "protein_id": "NP_001008213.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000378747.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001008212.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000378747.8",
          "protein_id": "ENSP00000368021.3",
          "transcript_support_level": 1,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001008212.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378747.8"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000378748.7",
          "protein_id": "ENSP00000368022.3",
          "transcript_support_level": 1,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378748.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000378757.6",
          "protein_id": "ENSP00000368032.2",
          "transcript_support_level": 1,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378757.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1424C>T",
          "hgvs_p": "p.Ala475Val",
          "transcript": "ENST00000378752.7",
          "protein_id": "ENSP00000368027.3",
          "transcript_support_level": 1,
          "aa_start": 475,
          "aa_end": null,
          "aa_length": 571,
          "cds_start": 1424,
          "cds_end": null,
          "cds_length": 1716,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378752.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1472C>T",
          "hgvs_p": "p.Ala491Val",
          "transcript": "ENST00000942709.1",
          "protein_id": "ENSP00000612768.1",
          "transcript_support_level": null,
          "aa_start": 491,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1472,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942709.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "NM_001008211.1",
          "protein_id": "NP_001008212.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001008211.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "NM_001008213.1",
          "protein_id": "NP_001008214.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001008213.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "NM_021980.4",
          "protein_id": "NP_068815.2",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_021980.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000263036.9",
          "protein_id": "ENSP00000263036.3",
          "transcript_support_level": 2,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000263036.9"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867524.1",
          "protein_id": "ENSP00000537583.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867524.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867525.1",
          "protein_id": "ENSP00000537584.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867525.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867527.1",
          "protein_id": "ENSP00000537586.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867527.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867528.1",
          "protein_id": "ENSP00000537587.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867528.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867531.1",
          "protein_id": "ENSP00000537590.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867531.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867535.1",
          "protein_id": "ENSP00000537594.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867535.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867536.1",
          "protein_id": "ENSP00000537595.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867536.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867539.1",
          "protein_id": "ENSP00000537598.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867539.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867545.1",
          "protein_id": "ENSP00000537604.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867545.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPTN",
          "gene_hgnc_id": 17142,
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val",
          "transcript": "ENST00000867548.1",
          "protein_id": "ENSP00000537607.1",
          "transcript_support_level": null,
          "aa_start": 481,
          "aa_end": null,
          "aa_length": 577,
          "cds_start": 1442,
          "cds_end": null,
          "cds_length": 1734,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        {
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          "biotype": "pseudogene",
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      ],
      "gene_symbol": "OPTN",
      "gene_hgnc_id": 17142,
      "dbsnp": "rs377219791",
      "frequency_reference_population": 0.00005455328,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 88,
      "gnomad_exomes_af": 0.0000568057,
      "gnomad_genomes_af": 0.0000328991,
      "gnomad_exomes_ac": 83,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8163797855377197,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.61,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.894,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.23,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.056,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 0,
          "pathogenic_score": 1,
          "criteria": [
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_021980.4",
          "gene_symbol": "OPTN",
          "hgnc_id": 17142,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD,SD",
          "hgvs_c": "c.1442C>T",
          "hgvs_p": "p.Ala481Val"
        }
      ],
      "clinvar_disease": " E, open angle,Amyotrophic lateral sclerosis type 12,Glaucoma 1,Inborn genetic diseases,OPTN-related disorder,Primary open angle glaucoma,not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:5",
      "phenotype_combined": "not specified|Amyotrophic lateral sclerosis type 12|Primary open angle glaucoma|Primary open angle glaucoma;Glaucoma 1, open angle, E;Amyotrophic lateral sclerosis type 12|Inborn genetic diseases|OPTN-related disorder",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}