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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-133362890-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=133362890&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 133362890,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_004092.4",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.851A>G",
          "hgvs_p": "p.Lys284Arg",
          "transcript": "NM_004092.4",
          "protein_id": "NP_004083.3",
          "transcript_support_level": null,
          "aa_start": 284,
          "aa_end": null,
          "aa_length": 290,
          "cds_start": 851,
          "cds_end": null,
          "cds_length": 873,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000368547.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004092.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.851A>G",
          "hgvs_p": "p.Lys284Arg",
          "transcript": "ENST00000368547.4",
          "protein_id": "ENSP00000357535.3",
          "transcript_support_level": 1,
          "aa_start": 284,
          "aa_end": null,
          "aa_length": 290,
          "cds_start": 851,
          "cds_end": null,
          "cds_length": 873,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004092.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000368547.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.1067A>G",
          "hgvs_p": "p.Lys356Arg",
          "transcript": "ENST00000857570.1",
          "protein_id": "ENSP00000527629.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 1067,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857570.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.1034A>G",
          "hgvs_p": "p.Lys345Arg",
          "transcript": "ENST00000970368.1",
          "protein_id": "ENSP00000640427.1",
          "transcript_support_level": null,
          "aa_start": 345,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 1034,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970368.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.884A>G",
          "hgvs_p": "p.Lys295Arg",
          "transcript": "ENST00000857564.1",
          "protein_id": "ENSP00000527623.1",
          "transcript_support_level": null,
          "aa_start": 295,
          "aa_end": null,
          "aa_length": 301,
          "cds_start": 884,
          "cds_end": null,
          "cds_length": 906,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857564.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.878A>G",
          "hgvs_p": "p.Lys293Arg",
          "transcript": "ENST00000857569.1",
          "protein_id": "ENSP00000527628.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 878,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857569.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.878A>G",
          "hgvs_p": "p.Lys293Arg",
          "transcript": "ENST00000857571.1",
          "protein_id": "ENSP00000527630.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 878,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857571.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.845A>G",
          "hgvs_p": "p.Lys282Arg",
          "transcript": "ENST00000857572.1",
          "protein_id": "ENSP00000527631.1",
          "transcript_support_level": null,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 845,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857572.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.845A>G",
          "hgvs_p": "p.Lys282Arg",
          "transcript": "ENST00000857573.1",
          "protein_id": "ENSP00000527632.1",
          "transcript_support_level": null,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 845,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857573.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.830A>G",
          "hgvs_p": "p.Lys277Arg",
          "transcript": "ENST00000935006.1",
          "protein_id": "ENSP00000605065.1",
          "transcript_support_level": null,
          "aa_start": 277,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 830,
          "cds_end": null,
          "cds_length": 852,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935006.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.824A>G",
          "hgvs_p": "p.Lys275Arg",
          "transcript": "ENST00000857567.1",
          "protein_id": "ENSP00000527626.1",
          "transcript_support_level": null,
          "aa_start": 275,
          "aa_end": null,
          "aa_length": 281,
          "cds_start": 824,
          "cds_end": null,
          "cds_length": 846,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857567.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.812A>G",
          "hgvs_p": "p.Lys271Arg",
          "transcript": "ENST00000857566.1",
          "protein_id": "ENSP00000527625.1",
          "transcript_support_level": null,
          "aa_start": 271,
          "aa_end": null,
          "aa_length": 277,
          "cds_start": 812,
          "cds_end": null,
          "cds_length": 834,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857566.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.731A>G",
          "hgvs_p": "p.Lys244Arg",
          "transcript": "ENST00000857565.1",
          "protein_id": "ENSP00000527624.1",
          "transcript_support_level": null,
          "aa_start": 244,
          "aa_end": null,
          "aa_length": 250,
          "cds_start": 731,
          "cds_end": null,
          "cds_length": 753,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857565.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.653A>G",
          "hgvs_p": "p.Lys218Arg",
          "transcript": "ENST00000857568.1",
          "protein_id": "ENSP00000527627.1",
          "transcript_support_level": null,
          "aa_start": 218,
          "aa_end": null,
          "aa_length": 224,
          "cds_start": 653,
          "cds_end": null,
          "cds_length": 675,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857568.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.623A>G",
          "hgvs_p": "p.Lys208Arg",
          "transcript": "ENST00000935007.1",
          "protein_id": "ENSP00000605066.1",
          "transcript_support_level": null,
          "aa_start": 208,
          "aa_end": null,
          "aa_length": 214,
          "cds_start": 623,
          "cds_end": null,
          "cds_length": 645,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935007.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.458A>G",
          "hgvs_p": "p.Lys153Arg",
          "transcript": "ENST00000857574.1",
          "protein_id": "ENSP00000527633.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 159,
          "cds_start": 458,
          "cds_end": null,
          "cds_length": 480,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857574.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "splice_acceptor_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.718-2A>G",
          "hgvs_p": null,
          "transcript": "ENST00000935008.1",
          "protein_id": "ENSP00000605067.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 245,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 738,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935008.1"
        }
      ],
      "gene_symbol": "ECHS1",
      "gene_hgnc_id": 3151,
      "dbsnp": "rs1261009123",
      "frequency_reference_population": 0.0000043368573,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 7,
      "gnomad_exomes_af": 0.00000410447,
      "gnomad_genomes_af": 0.00000656806,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3198680877685547,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.124,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.104,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 6.635,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM1,PM2,PP2,BP4",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 1,
          "pathogenic_score": 5,
          "criteria": [
            "PM1",
            "PM2",
            "PP2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_004092.4",
          "gene_symbol": "ECHS1",
          "hgnc_id": 3151,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.851A>G",
          "hgvs_p": "p.Lys284Arg"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}