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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-133362910-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=133362910&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 133362910,
      "ref": "G",
      "alt": "A",
      "effect": "synonymous_variant",
      "transcript": "NM_004092.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.831C>T",
          "hgvs_p": "p.Thr277Thr",
          "transcript": "NM_004092.4",
          "protein_id": "NP_004083.3",
          "transcript_support_level": null,
          "aa_start": 277,
          "aa_end": null,
          "aa_length": 290,
          "cds_start": 831,
          "cds_end": null,
          "cds_length": 873,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000368547.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004092.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.831C>T",
          "hgvs_p": "p.Thr277Thr",
          "transcript": "ENST00000368547.4",
          "protein_id": "ENSP00000357535.3",
          "transcript_support_level": 1,
          "aa_start": 277,
          "aa_end": null,
          "aa_length": 290,
          "cds_start": 831,
          "cds_end": null,
          "cds_length": 873,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004092.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000368547.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.1047C>T",
          "hgvs_p": "p.Thr349Thr",
          "transcript": "ENST00000857570.1",
          "protein_id": "ENSP00000527629.1",
          "transcript_support_level": null,
          "aa_start": 349,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 1047,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857570.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.1014C>T",
          "hgvs_p": "p.Thr338Thr",
          "transcript": "ENST00000970368.1",
          "protein_id": "ENSP00000640427.1",
          "transcript_support_level": null,
          "aa_start": 338,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 1014,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970368.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.864C>T",
          "hgvs_p": "p.Thr288Thr",
          "transcript": "ENST00000857564.1",
          "protein_id": "ENSP00000527623.1",
          "transcript_support_level": null,
          "aa_start": 288,
          "aa_end": null,
          "aa_length": 301,
          "cds_start": 864,
          "cds_end": null,
          "cds_length": 906,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857564.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.858C>T",
          "hgvs_p": "p.Thr286Thr",
          "transcript": "ENST00000857569.1",
          "protein_id": "ENSP00000527628.1",
          "transcript_support_level": null,
          "aa_start": 286,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 858,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857569.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.858C>T",
          "hgvs_p": "p.Thr286Thr",
          "transcript": "ENST00000857571.1",
          "protein_id": "ENSP00000527630.1",
          "transcript_support_level": null,
          "aa_start": 286,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 858,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857571.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.825C>T",
          "hgvs_p": "p.Thr275Thr",
          "transcript": "ENST00000857572.1",
          "protein_id": "ENSP00000527631.1",
          "transcript_support_level": null,
          "aa_start": 275,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 825,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857572.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.825C>T",
          "hgvs_p": "p.Thr275Thr",
          "transcript": "ENST00000857573.1",
          "protein_id": "ENSP00000527632.1",
          "transcript_support_level": null,
          "aa_start": 275,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 825,
          "cds_end": null,
          "cds_length": 867,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857573.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.810C>T",
          "hgvs_p": "p.Thr270Thr",
          "transcript": "ENST00000935006.1",
          "protein_id": "ENSP00000605065.1",
          "transcript_support_level": null,
          "aa_start": 270,
          "aa_end": null,
          "aa_length": 283,
          "cds_start": 810,
          "cds_end": null,
          "cds_length": 852,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935006.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.804C>T",
          "hgvs_p": "p.Thr268Thr",
          "transcript": "ENST00000857567.1",
          "protein_id": "ENSP00000527626.1",
          "transcript_support_level": null,
          "aa_start": 268,
          "aa_end": null,
          "aa_length": 281,
          "cds_start": 804,
          "cds_end": null,
          "cds_length": 846,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857567.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.792C>T",
          "hgvs_p": "p.Thr264Thr",
          "transcript": "ENST00000857566.1",
          "protein_id": "ENSP00000527625.1",
          "transcript_support_level": null,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 277,
          "cds_start": 792,
          "cds_end": null,
          "cds_length": 834,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857566.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.711C>T",
          "hgvs_p": "p.Thr237Thr",
          "transcript": "ENST00000857565.1",
          "protein_id": "ENSP00000527624.1",
          "transcript_support_level": null,
          "aa_start": 237,
          "aa_end": null,
          "aa_length": 250,
          "cds_start": 711,
          "cds_end": null,
          "cds_length": 753,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857565.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.633C>T",
          "hgvs_p": "p.Thr211Thr",
          "transcript": "ENST00000857568.1",
          "protein_id": "ENSP00000527627.1",
          "transcript_support_level": null,
          "aa_start": 211,
          "aa_end": null,
          "aa_length": 224,
          "cds_start": 633,
          "cds_end": null,
          "cds_length": 675,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857568.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.603C>T",
          "hgvs_p": "p.Thr201Thr",
          "transcript": "ENST00000935007.1",
          "protein_id": "ENSP00000605066.1",
          "transcript_support_level": null,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 214,
          "cds_start": 603,
          "cds_end": null,
          "cds_length": 645,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935007.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.438C>T",
          "hgvs_p": "p.Thr146Thr",
          "transcript": "ENST00000857574.1",
          "protein_id": "ENSP00000527633.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 159,
          "cds_start": 438,
          "cds_end": null,
          "cds_length": 480,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857574.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "ECHS1",
          "gene_hgnc_id": 3151,
          "hgvs_c": "c.718-22C>T",
          "hgvs_p": null,
          "transcript": "ENST00000935008.1",
          "protein_id": "ENSP00000605067.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 245,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 738,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935008.1"
        }
      ],
      "gene_symbol": "ECHS1",
      "gene_hgnc_id": 3151,
      "dbsnp": "rs769725255",
      "frequency_reference_population": 0.000014869944,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 24,
      "gnomad_exomes_af": 0.0000136818,
      "gnomad_genomes_af": 0.0000262819,
      "gnomad_exomes_ac": 20,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.8799999952316284,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.88,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -3.41,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong,BP6_Moderate,BP7",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 7,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_004092.4",
          "gene_symbol": "ECHS1",
          "hgnc_id": 3151,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.831C>T",
          "hgvs_p": "p.Thr277Thr"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}