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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-6464322-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=6464322&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 6464322,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_006257.5",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "NM_006257.5",
          "protein_id": "NP_006248.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000263125.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006257.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000263125.10",
          "protein_id": "ENSP00000263125.5",
          "transcript_support_level": 1,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_006257.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000263125.10"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000915286.1",
          "protein_id": "ENSP00000585345.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 714,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2145,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000915286.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "NM_001323265.1",
          "protein_id": "NP_001310194.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001323265.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000866196.1",
          "protein_id": "ENSP00000536255.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866196.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000866197.1",
          "protein_id": "ENSP00000536256.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866197.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000866198.1",
          "protein_id": "ENSP00000536257.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866198.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000866199.1",
          "protein_id": "ENSP00000536258.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000866199.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000915287.1",
          "protein_id": "ENSP00000585346.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000915287.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000915288.1",
          "protein_id": "ENSP00000585347.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000915288.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000955425.1",
          "protein_id": "ENSP00000625484.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955425.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1328C>G",
          "hgvs_p": "p.Ser443Cys",
          "transcript": "NM_001282644.2",
          "protein_id": "NP_001269573.1",
          "transcript_support_level": null,
          "aa_start": 443,
          "aa_end": null,
          "aa_length": 670,
          "cds_start": 1328,
          "cds_end": null,
          "cds_length": 2013,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001282644.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000915285.1",
          "protein_id": "ENSP00000585344.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000915285.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000955428.1",
          "protein_id": "ENSP00000625487.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000955428.1"
        },
        {
          "aa_ref": "S",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_count": 17,
          "intron_rank": null,
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          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "NM_001242413.2",
          "protein_id": "NP_001229342.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 643,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 1932,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001242413.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000397176.6",
          "protein_id": "ENSP00000380361.2",
          "transcript_support_level": 5,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 643,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 1932,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000397176.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000955426.1",
          "protein_id": "ENSP00000625485.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 643,
          "cds_start": 1436,
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          "cdna_start": null,
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        },
        {
          "aa_ref": "S",
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          "strand": false,
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          ],
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          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1328C>G",
          "hgvs_p": "p.Ser443Cys",
          "transcript": "NM_001323267.2",
          "protein_id": "NP_001310196.1",
          "transcript_support_level": null,
          "aa_start": 443,
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          "aa_length": 627,
          "cds_start": 1328,
          "cds_end": null,
          "cds_length": 1884,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001323267.2"
        },
        {
          "aa_ref": "S",
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          "protein_coding": true,
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          "consequences": [
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          ],
          "exon_rank": 13,
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          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys",
          "transcript": "ENST00000955427.1",
          "protein_id": "ENSP00000625486.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 600,
          "cds_start": 1436,
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          "cds_length": 1803,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000955427.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PRKCQ",
          "gene_hgnc_id": 9410,
          "hgvs_c": "c.1061C>G",
          "hgvs_p": "p.Ser354Cys",
          "transcript": "NM_001282645.1",
          "protein_id": "NP_001269574.1",
          "transcript_support_level": null,
          "aa_start": 354,
          "aa_end": null,
          "aa_length": 581,
          "cds_start": 1061,
          "cds_end": null,
          "cds_length": 1746,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
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          "feature": "NM_001282645.1"
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          "transcript": "ENST00000955424.1",
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          "transcript": "XM_005252496.5",
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        {
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          ],
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          "gene_symbol": "PRKCQ",
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          "hgvs_c": "c.1538C>G",
          "hgvs_p": "p.Ser513Cys",
          "transcript": "XM_005252497.5",
          "protein_id": "XP_005252554.1",
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          "biotype": "protein_coding",
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        },
        {
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            "intron_variant"
          ],
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          "exon_count": 3,
          "intron_rank": 2,
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          "gene_symbol": "ENSG00000302067",
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          "hgvs_c": "n.381+17350G>C",
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          "transcript": "ENST00000783835.1",
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          "transcript_support_level": null,
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          "mane_select": null,
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          "biotype": "pseudogene",
          "feature": "ENST00000783835.1"
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      ],
      "gene_symbol": "PRKCQ",
      "gene_hgnc_id": 9410,
      "dbsnp": "rs78855257",
      "frequency_reference_population": 0.0013434547,
      "hom_count_reference_population": 29,
      "allele_count_reference_population": 2168,
      "gnomad_exomes_af": 0.000756109,
      "gnomad_genomes_af": 0.00697873,
      "gnomad_exomes_ac": 1105,
      "gnomad_genomes_ac": 1063,
      "gnomad_exomes_homalt": 13,
      "gnomad_genomes_homalt": 16,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.007692873477935791,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.217,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0576,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.31,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.045,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_006257.5",
          "gene_symbol": "PRKCQ",
          "hgnc_id": 9410,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1436C>G",
          "hgvs_p": "p.Ser479Cys"
        },
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000783835.1",
          "gene_symbol": "ENSG00000302067",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.381+17350G>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:2",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}