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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-69398758-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=69398758&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 69398758,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000359426.7",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2551G>A",
          "hgvs_p": "p.Glu851Lys",
          "transcript": "NM_001358263.1",
          "protein_id": "NP_001345192.1",
          "transcript_support_level": null,
          "aa_start": 851,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2551,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": 3036,
          "cdna_end": null,
          "cdna_length": 3998,
          "mane_select": null,
          "mane_plus": "ENST00000643399.2",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2551G>A",
          "hgvs_p": "p.Glu851Lys",
          "transcript": "ENST00000643399.2",
          "protein_id": "ENSP00000494664.1",
          "transcript_support_level": null,
          "aa_start": 851,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2551,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": 3036,
          "cdna_end": null,
          "cdna_length": 3998,
          "mane_select": null,
          "mane_plus": "NM_001358263.1",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2539G>A",
          "hgvs_p": "p.Glu847Lys",
          "transcript": "NM_000188.3",
          "protein_id": "NP_000179.2",
          "transcript_support_level": null,
          "aa_start": 847,
          "aa_end": null,
          "aa_length": 917,
          "cds_start": 2539,
          "cds_end": null,
          "cds_length": 2754,
          "cdna_start": 2640,
          "cdna_end": null,
          "cdna_length": 3602,
          "mane_select": "ENST00000359426.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2539G>A",
          "hgvs_p": "p.Glu847Lys",
          "transcript": "ENST00000359426.7",
          "protein_id": "ENSP00000352398.6",
          "transcript_support_level": 1,
          "aa_start": 847,
          "aa_end": null,
          "aa_length": 917,
          "cds_start": 2539,
          "cds_end": null,
          "cds_length": 2754,
          "cdna_start": 2640,
          "cdna_end": null,
          "cdna_length": 3602,
          "mane_select": "NM_000188.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2644G>A",
          "hgvs_p": "p.Glu882Lys",
          "transcript": "NM_001322365.2",
          "protein_id": "NP_001309294.1",
          "transcript_support_level": null,
          "aa_start": 882,
          "aa_end": null,
          "aa_length": 952,
          "cds_start": 2644,
          "cds_end": null,
          "cds_length": 2859,
          "cdna_start": 3033,
          "cdna_end": null,
          "cdna_length": 3995,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2551G>A",
          "hgvs_p": "p.Glu851Lys",
          "transcript": "NM_001322364.2",
          "protein_id": "NP_001309293.1",
          "transcript_support_level": null,
          "aa_start": 851,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2551,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": 2728,
          "cdna_end": null,
          "cdna_length": 3690,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2551G>A",
          "hgvs_p": "p.Glu851Lys",
          "transcript": "NM_033497.3",
          "protein_id": "NP_277032.1",
          "transcript_support_level": null,
          "aa_start": 851,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2551,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": 2850,
          "cdna_end": null,
          "cdna_length": 3812,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2551G>A",
          "hgvs_p": "p.Glu851Lys",
          "transcript": "NM_033498.3",
          "protein_id": "NP_277033.1",
          "transcript_support_level": null,
          "aa_start": 851,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2551,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": 2904,
          "cdna_end": null,
          "cdna_length": 3866,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2551G>A",
          "hgvs_p": "p.Glu851Lys",
          "transcript": "ENST00000436817.6",
          "protein_id": "ENSP00000415949.2",
          "transcript_support_level": 5,
          "aa_start": 851,
          "aa_end": null,
          "aa_length": 921,
          "cds_start": 2551,
          "cds_end": null,
          "cds_length": 2766,
          "cdna_start": 2871,
          "cdna_end": null,
          "cdna_length": 3835,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2536G>A",
          "hgvs_p": "p.Glu846Lys",
          "transcript": "NM_001441139.1",
          "protein_id": "NP_001428068.1",
          "transcript_support_level": null,
          "aa_start": 846,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2536,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": 2637,
          "cdna_end": null,
          "cdna_length": 3599,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2536G>A",
          "hgvs_p": "p.Glu846Lys",
          "transcript": "NM_033496.3",
          "protein_id": "NP_277031.1",
          "transcript_support_level": null,
          "aa_start": 846,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2536,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": 2722,
          "cdna_end": null,
          "cdna_length": 3684,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2536G>A",
          "hgvs_p": "p.Glu846Lys",
          "transcript": "ENST00000298649.8",
          "protein_id": "ENSP00000298649.3",
          "transcript_support_level": 5,
          "aa_start": 846,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2536,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": 2706,
          "cdna_end": null,
          "cdna_length": 3229,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2530G>A",
          "hgvs_p": "p.Glu844Lys",
          "transcript": "NM_001441140.1",
          "protein_id": "NP_001428069.1",
          "transcript_support_level": null,
          "aa_start": 844,
          "aa_end": null,
          "aa_length": 914,
          "cds_start": 2530,
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          "cds_length": 2745,
          "cdna_start": 2631,
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          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2539G>A",
          "hgvs_p": "p.Glu847Lys",
          "transcript": "NM_001441141.1",
          "protein_id": "NP_001428070.1",
          "transcript_support_level": null,
          "aa_start": 847,
          "aa_end": null,
          "aa_length": 914,
          "cds_start": 2539,
          "cds_end": null,
          "cds_length": 2745,
          "cdna_start": 2640,
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          "cdna_length": 3593,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
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            "missense_variant"
          ],
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          "intron_rank": null,
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          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2503G>A",
          "hgvs_p": "p.Glu835Lys",
          "transcript": "NM_033500.2",
          "protein_id": "NP_277035.2",
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          "aa_start": 835,
          "aa_end": null,
          "aa_length": 905,
          "cds_start": 2503,
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          "cdna_start": 3002,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2497G>A",
          "hgvs_p": "p.Glu833Lys",
          "transcript": "NM_001441142.1",
          "protein_id": "NP_001428071.1",
          "transcript_support_level": null,
          "aa_start": 833,
          "aa_end": null,
          "aa_length": 903,
          "cds_start": 2497,
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          "cds_length": 2712,
          "cdna_start": 2786,
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          "cdna_length": 3748,
          "mane_select": null,
          "mane_plus": null,
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          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2455G>A",
          "hgvs_p": "p.Glu819Lys",
          "transcript": "NM_001322366.1",
          "protein_id": "NP_001309295.1",
          "transcript_support_level": null,
          "aa_start": 819,
          "aa_end": null,
          "aa_length": 889,
          "cds_start": 2455,
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          "cds_length": 2670,
          "cdna_start": 2531,
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          "cdna_length": 3493,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 18,
          "intron_rank": null,
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          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2455G>A",
          "hgvs_p": "p.Glu819Lys",
          "transcript": "NM_001441143.1",
          "protein_id": "NP_001428072.1",
          "transcript_support_level": null,
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          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
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          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2455G>A",
          "hgvs_p": "p.Glu819Lys",
          "transcript": "ENST00000703945.1",
          "protein_id": "ENSP00000515578.1",
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          "aa_start": 819,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HK1",
          "gene_hgnc_id": 4922,
          "hgvs_c": "c.2443G>A",
          "hgvs_p": "p.Glu815Lys",
          "transcript": "NM_001322367.1",
          "protein_id": "NP_001309296.1",
          "transcript_support_level": null,
          "aa_start": 815,
          "aa_end": null,
          "aa_length": 885,
          "cds_start": 2443,
          "cds_end": null,
          "cds_length": 2658,
          "cdna_start": 2544,
          "cdna_end": null,
          "cdna_length": 3506,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
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        },
        {
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          "protein_id": "ENSP00000515587.1",
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        }
      ],
      "gene_symbol": "HK1",
      "gene_hgnc_id": 4922,
      "dbsnp": "rs777849213",
      "frequency_reference_population": 0.0000020521493,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205215,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.652859091758728,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.819,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.2682,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.4,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 10.003,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 10,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 10,
          "benign_score": 0,
          "pathogenic_score": 10,
          "criteria": [
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000359426.7",
          "gene_symbol": "HK1",
          "hgnc_id": 4922,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.2539G>A",
          "hgvs_p": "p.Glu847Lys"
        }
      ],
      "clinvar_disease": "HK1-related disorder,Retinal dystrophy,Retinitis pigmentosa 79,See cases,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:5 LP:5",
      "phenotype_combined": "Retinitis pigmentosa 79|not provided|Retinal dystrophy|See cases|HK1-related disorder",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}