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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-76889496-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=76889496&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 76889496,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000286628.14",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3416G>A",
          "hgvs_p": "p.Arg1139Gln",
          "transcript": "NM_001161352.2",
          "protein_id": "NP_001154824.1",
          "transcript_support_level": null,
          "aa_start": 1139,
          "aa_end": null,
          "aa_length": 1236,
          "cds_start": 3416,
          "cds_end": null,
          "cds_length": 3711,
          "cdna_start": 3582,
          "cdna_end": null,
          "cdna_length": 6261,
          "mane_select": "ENST00000286628.14",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3416G>A",
          "hgvs_p": "p.Arg1139Gln",
          "transcript": "ENST00000286628.14",
          "protein_id": "ENSP00000286628.8",
          "transcript_support_level": 1,
          "aa_start": 1139,
          "aa_end": null,
          "aa_length": 1236,
          "cds_start": 3416,
          "cds_end": null,
          "cds_length": 3711,
          "cdna_start": 3582,
          "cdna_end": null,
          "cdna_length": 6261,
          "mane_select": "NM_001161352.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3365G>A",
          "hgvs_p": "p.Arg1122Gln",
          "transcript": "ENST00000626620.3",
          "protein_id": "ENSP00000485867.1",
          "transcript_support_level": 1,
          "aa_start": 1122,
          "aa_end": null,
          "aa_length": 1219,
          "cds_start": 3365,
          "cds_end": null,
          "cds_length": 3660,
          "cdna_start": 3365,
          "cdna_end": null,
          "cdna_length": 3660,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3332G>A",
          "hgvs_p": "p.Arg1111Gln",
          "transcript": "ENST00000639406.1",
          "protein_id": "ENSP00000491732.1",
          "transcript_support_level": 1,
          "aa_start": 1111,
          "aa_end": null,
          "aa_length": 1208,
          "cds_start": 3332,
          "cds_end": null,
          "cds_length": 3627,
          "cdna_start": 3412,
          "cdna_end": null,
          "cdna_length": 5567,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3242G>A",
          "hgvs_p": "p.Arg1081Gln",
          "transcript": "ENST00000286627.10",
          "protein_id": "ENSP00000286627.5",
          "transcript_support_level": 1,
          "aa_start": 1081,
          "aa_end": null,
          "aa_length": 1178,
          "cds_start": 3242,
          "cds_end": null,
          "cds_length": 3537,
          "cdna_start": 3502,
          "cdna_end": null,
          "cdna_length": 6194,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3092G>A",
          "hgvs_p": "p.Arg1031Gln",
          "transcript": "ENST00000640807.1",
          "protein_id": "ENSP00000491555.1",
          "transcript_support_level": 1,
          "aa_start": 1031,
          "aa_end": null,
          "aa_length": 1128,
          "cds_start": 3092,
          "cds_end": null,
          "cds_length": 3387,
          "cdna_start": 3092,
          "cdna_end": null,
          "cdna_length": 3387,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.2837G>A",
          "hgvs_p": "p.Arg946Gln",
          "transcript": "ENST00000604624.6",
          "protein_id": "ENSP00000473714.2",
          "transcript_support_level": 1,
          "aa_start": 946,
          "aa_end": null,
          "aa_length": 1043,
          "cds_start": 2837,
          "cds_end": null,
          "cds_length": 3132,
          "cdna_start": 2837,
          "cdna_end": null,
          "cdna_length": 11400,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "n.*2121G>A",
          "hgvs_p": null,
          "transcript": "ENST00000639691.1",
          "protein_id": "ENSP00000491040.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3145,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "n.3466G>A",
          "hgvs_p": null,
          "transcript": "ENST00000639851.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3866,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "n.*2121G>A",
          "hgvs_p": null,
          "transcript": "ENST00000639691.1",
          "protein_id": "ENSP00000491040.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3145,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1-AS1",
          "gene_hgnc_id": 51213,
          "hgvs_c": "n.238+1215C>T",
          "hgvs_p": null,
          "transcript": "ENST00000429850.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3566,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3416G>A",
          "hgvs_p": "p.Arg1139Gln",
          "transcript": "ENST00000404771.8",
          "protein_id": "ENSP00000385717.3",
          "transcript_support_level": 5,
          "aa_start": 1139,
          "aa_end": null,
          "aa_length": 1288,
          "cds_start": 3416,
          "cds_end": null,
          "cds_length": 3867,
          "cdna_start": 3549,
          "cdna_end": null,
          "cdna_length": 4014,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3425G>A",
          "hgvs_p": "p.Arg1142Gln",
          "transcript": "ENST00000639591.1",
          "protein_id": "ENSP00000492793.1",
          "transcript_support_level": 5,
          "aa_start": 1142,
          "aa_end": null,
          "aa_length": 1246,
          "cds_start": 3425,
          "cds_end": null,
          "cds_length": 3741,
          "cdna_start": 3558,
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          "cdna_length": 3888,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3437G>A",
          "hgvs_p": "p.Arg1146Gln",
          "transcript": "ENST00000640523.1",
          "protein_id": "ENSP00000491795.1",
          "transcript_support_level": 5,
          "aa_start": 1146,
          "aa_end": null,
          "aa_length": 1242,
          "cds_start": 3437,
          "cds_end": null,
          "cds_length": 3729,
          "cdna_start": 3910,
          "cdna_end": null,
          "cdna_length": 5425,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3428G>A",
          "hgvs_p": "p.Arg1143Gln",
          "transcript": "ENST00000638759.1",
          "protein_id": "ENSP00000492632.1",
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          "aa_start": 1143,
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          "aa_length": 1240,
          "cds_start": 3428,
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          "cdna_start": 3428,
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          "cdna_length": 3723,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3425G>A",
          "hgvs_p": "p.Arg1142Gln",
          "transcript": "ENST00000638575.1",
          "protein_id": "ENSP00000492049.1",
          "transcript_support_level": 5,
          "aa_start": 1142,
          "aa_end": null,
          "aa_length": 1239,
          "cds_start": 3425,
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          "cds_length": 3720,
          "cdna_start": 3425,
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          "cdna_length": 3720,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3428G>A",
          "hgvs_p": "p.Arg1143Gln",
          "transcript": "ENST00000638848.1",
          "protein_id": "ENSP00000492414.1",
          "transcript_support_level": 5,
          "aa_start": 1143,
          "aa_end": null,
          "aa_length": 1239,
          "cds_start": 3428,
          "cds_end": null,
          "cds_length": 3720,
          "cdna_start": 3508,
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          "cdna_length": 4423,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3263G>A",
          "hgvs_p": "p.Arg1088Gln",
          "transcript": "ENST00000640605.1",
          "protein_id": "ENSP00000491435.1",
          "transcript_support_level": 5,
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          "cds_start": 3263,
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          "cdna_start": 3396,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3416G>A",
          "hgvs_p": "p.Arg1139Gln",
          "transcript": "ENST00000639544.1",
          "protein_id": "ENSP00000492075.1",
          "transcript_support_level": 5,
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          "aa_length": 1236,
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          "cdna_start": 3416,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNMA1",
          "gene_hgnc_id": 6284,
          "hgvs_c": "c.3416G>A",
          "hgvs_p": "p.Arg1139Gln",
          "transcript": "ENST00000457953.6",
          "protein_id": "ENSP00000396608.2",
          "transcript_support_level": 5,
          "aa_start": 1139,
          "aa_end": null,
          "aa_length": 1235,
          "cds_start": 3416,
          "cds_end": null,
          "cds_length": 3708,
          "cdna_start": 3540,
          "cdna_end": null,
          "cdna_length": 3976,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
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      ],
      "gene_symbol": "KCNMA1",
      "gene_hgnc_id": 6284,
      "dbsnp": "rs1397556923",
      "frequency_reference_population": 0.0000030981353,
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      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000273633,
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      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8931909203529358,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.3400000035762787,
      "splice_prediction_selected": "Uncertain_significance",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.661,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.8982,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.29,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.568,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.34,
      "spliceai_max_prediction": "Uncertain_significance",
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      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000286628.14",
          "gene_symbol": "KCNMA1",
          "hgnc_id": 6284,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.3416G>A",
          "hgvs_p": "p.Arg1139Gln"
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        {
          "score": 4,
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          "criteria": [
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            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000429850.2",
          "gene_symbol": "KCNMA1-AS1",
          "hgnc_id": 51213,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.238+1215C>T",
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      ],
      "clinvar_disease": "Generalized epilepsy-paroxysmal dyskinesia syndrome",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Generalized epilepsy-paroxysmal dyskinesia syndrome",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}