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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-78009515-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=78009515&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 78009515,
      "ref": "C",
      "alt": "T",
      "effect": "intron_variant",
      "transcript": "ENST00000372371.8",
      "consequences": [
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "c.1909+22G>A",
          "hgvs_p": null,
          "transcript": "NM_007055.4",
          "protein_id": "NP_008986.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1390,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 4173,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6610,
          "mane_select": "ENST00000372371.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "c.1909+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000372371.8",
          "protein_id": "ENSP00000361446.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1390,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 4173,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6610,
          "mane_select": "NM_007055.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": 13,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "c.1768+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698731.1",
          "protein_id": "ENSP00000513898.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1343,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 4032,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6456,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.131+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000472014.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 699,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.570+349G>A",
          "hgvs_p": null,
          "transcript": "ENST00000473588.2",
          "protein_id": "ENSP00000473389.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1022,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.1488+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698728.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6068,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.3034+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698729.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7516,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.3034+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698730.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7614,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.*770+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698732.1",
          "protein_id": "ENSP00000513899.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6519,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": 15,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.*1096+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698733.1",
          "protein_id": "ENSP00000513900.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7075,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.1909+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698734.1",
          "protein_id": "ENSP00000513901.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7411,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.2024+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698735.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6840,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.2024+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698736.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7253,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.2024+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698737.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4196,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.2024+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698738.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3169,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "POLR3A",
          "gene_hgnc_id": 30074,
          "hgvs_c": "n.2024+22G>A",
          "hgvs_p": null,
          "transcript": "ENST00000698739.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3006,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "POLR3A",
      "gene_hgnc_id": 30074,
      "dbsnp": "rs191875469",
      "frequency_reference_population": 0.0024086204,
      "hom_count_reference_population": 10,
      "allele_count_reference_population": 3888,
      "gnomad_exomes_af": 0.00249889,
      "gnomad_genomes_af": 0.00154248,
      "gnomad_exomes_ac": 3653,
      "gnomad_genomes_ac": 235,
      "gnomad_exomes_homalt": 10,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.7900000214576721,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.10000000149011612,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.79,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.768,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.1,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PP5_Very_Strong,BP4,BS2_Supporting",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 2,
          "pathogenic_score": 8,
          "criteria": [
            "PP5_Very_Strong",
            "BP4",
            "BS2_Supporting"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000372371.8",
          "gene_symbol": "POLR3A",
          "hgnc_id": 30074,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1909+22G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " ataxia, hypodontia, hypomyelination syndrome,Hereditary ataxia,Hypomyelinating leukodystrophy 8 with or without oligodontia and-or hypogonadotropic hypogonadism,Inborn genetic diseases,Leukoencephalopathy,Movement disorder,Neonatal pseudo-hydrocephalic progeroid syndrome,POLR-related leukodystrophy,POLR3A-related disorder,POLR3A-related neurological disorders,Spastic ataxia,not provided,not specified",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:22 LP:4 US:2 O:2",
      "phenotype_combined": "not provided|Neonatal pseudo-hydrocephalic progeroid syndrome|Leukoencephalopathy, ataxia, hypodontia, hypomyelination syndrome|POLR3A-related neurological disorders|not specified|POLR3A-related disorder|Hypomyelinating leukodystrophy 8 with or without oligodontia and-or hypogonadotropic hypogonadism;Leukoencephalopathy, ataxia, hypodontia, hypomyelination syndrome;Neonatal pseudo-hydrocephalic progeroid syndrome|Inborn genetic diseases|POLR-related leukodystrophy|Spastic ataxia|Movement disorder|Hereditary ataxia",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}