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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-95633012-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=95633012&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 95633012,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000371224.7",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.755G>A",
          "hgvs_p": "p.Arg252Gln",
          "transcript": "NM_002860.4",
          "protein_id": "NP_002851.2",
          "transcript_support_level": null,
          "aa_start": 252,
          "aa_end": null,
          "aa_length": 795,
          "cds_start": 755,
          "cds_end": null,
          "cds_length": 2388,
          "cdna_start": 898,
          "cdna_end": null,
          "cdna_length": 3352,
          "mane_select": "ENST00000371224.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.755G>A",
          "hgvs_p": "p.Arg252Gln",
          "transcript": "ENST00000371224.7",
          "protein_id": "ENSP00000360268.2",
          "transcript_support_level": 1,
          "aa_start": 252,
          "aa_end": null,
          "aa_length": 795,
          "cds_start": 755,
          "cds_end": null,
          "cds_length": 2388,
          "cdna_start": 898,
          "cdna_end": null,
          "cdna_length": 3352,
          "mane_select": "NM_002860.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.749G>A",
          "hgvs_p": "p.Arg250Gln",
          "transcript": "ENST00000371221.3",
          "protein_id": "ENSP00000360265.3",
          "transcript_support_level": 1,
          "aa_start": 250,
          "aa_end": null,
          "aa_length": 793,
          "cds_start": 749,
          "cds_end": null,
          "cds_length": 2382,
          "cdna_start": 887,
          "cdna_end": null,
          "cdna_length": 3337,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.755G>A",
          "hgvs_p": "p.Arg252Gln",
          "transcript": "NM_001323413.2",
          "protein_id": "NP_001310342.1",
          "transcript_support_level": null,
          "aa_start": 252,
          "aa_end": null,
          "aa_length": 795,
          "cds_start": 755,
          "cds_end": null,
          "cds_length": 2388,
          "cdna_start": 1049,
          "cdna_end": null,
          "cdna_length": 3503,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.755G>A",
          "hgvs_p": "p.Arg252Gln",
          "transcript": "NM_001323414.2",
          "protein_id": "NP_001310343.1",
          "transcript_support_level": null,
          "aa_start": 252,
          "aa_end": null,
          "aa_length": 795,
          "cds_start": 755,
          "cds_end": null,
          "cds_length": 2388,
          "cdna_start": 1206,
          "cdna_end": null,
          "cdna_length": 3660,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.749G>A",
          "hgvs_p": "p.Arg250Gln",
          "transcript": "NM_001017423.2",
          "protein_id": "NP_001017423.1",
          "transcript_support_level": null,
          "aa_start": 250,
          "aa_end": null,
          "aa_length": 793,
          "cds_start": 749,
          "cds_end": null,
          "cds_length": 2382,
          "cdna_start": 892,
          "cdna_end": null,
          "cdna_length": 3346,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.749G>A",
          "hgvs_p": "p.Arg250Gln",
          "transcript": "NM_001323415.2",
          "protein_id": "NP_001310344.1",
          "transcript_support_level": null,
          "aa_start": 250,
          "aa_end": null,
          "aa_length": 793,
          "cds_start": 749,
          "cds_end": null,
          "cds_length": 2382,
          "cdna_start": 1043,
          "cdna_end": null,
          "cdna_length": 3497,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.650G>A",
          "hgvs_p": "p.Arg217Gln",
          "transcript": "NM_001323417.2",
          "protein_id": "NP_001310346.1",
          "transcript_support_level": null,
          "aa_start": 217,
          "aa_end": null,
          "aa_length": 760,
          "cds_start": 650,
          "cds_end": null,
          "cds_length": 2283,
          "cdna_start": 793,
          "cdna_end": null,
          "cdna_length": 3247,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.422G>A",
          "hgvs_p": "p.Arg141Gln",
          "transcript": "NM_001323412.2",
          "protein_id": "NP_001310341.1",
          "transcript_support_level": null,
          "aa_start": 141,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 422,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": 683,
          "cdna_end": null,
          "cdna_length": 3137,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.422G>A",
          "hgvs_p": "p.Arg141Gln",
          "transcript": "NM_001323416.2",
          "protein_id": "NP_001310345.1",
          "transcript_support_level": null,
          "aa_start": 141,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 422,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": 834,
          "cdna_end": null,
          "cdna_length": 3288,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.416G>A",
          "hgvs_p": "p.Arg139Gln",
          "transcript": "NM_001323418.2",
          "protein_id": "NP_001310347.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 682,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 2049,
          "cdna_start": 828,
          "cdna_end": null,
          "cdna_length": 3282,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH18A1",
          "gene_hgnc_id": 9722,
          "hgvs_c": "c.119G>A",
          "hgvs_p": "p.Arg40Gln",
          "transcript": "NM_001323419.2",
          "protein_id": "NP_001310348.1",
          "transcript_support_level": null,
          "aa_start": 40,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 119,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": 428,
          "cdna_end": null,
          "cdna_length": 2882,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "ALDH18A1",
      "gene_hgnc_id": 9722,
      "dbsnp": "rs864321670",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7512038946151733,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.09000000357627869,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.433,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.2211,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.04,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.457,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.09,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 11,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PP3,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 11,
          "benign_score": 0,
          "pathogenic_score": 11,
          "criteria": [
            "PM2",
            "PP3",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000371224.7",
          "gene_symbol": "ALDH18A1",
          "hgnc_id": 9722,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD,SD",
          "hgvs_c": "c.755G>A",
          "hgvs_p": "p.Arg252Gln"
        }
      ],
      "clinvar_disease": " autosomal dominant 3,ALDH18A1 deficiency,Autosomal dominant spastic paraplegia type 9,Cutis laxa,Hereditary spastic paraplegia 9A,de Barsy syndrome,not provided",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:6",
      "phenotype_combined": "Hereditary spastic paraplegia 9A|ALDH18A1 deficiency|not provided|Cutis laxa, autosomal dominant 3;Autosomal dominant spastic paraplegia type 9;de Barsy syndrome",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}