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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 11-113977879-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=11&pos=113977879&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "11",
      "pos": 113977879,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_213621.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.176C>T",
          "hgvs_p": "p.Thr59Ile",
          "transcript": "NM_000869.6",
          "protein_id": "NP_000860.3",
          "transcript_support_level": null,
          "aa_start": 59,
          "aa_end": null,
          "aa_length": 478,
          "cds_start": 176,
          "cds_end": null,
          "cds_length": 1437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000504030.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000869.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.176C>T",
          "hgvs_p": "p.Thr59Ile",
          "transcript": "ENST00000504030.7",
          "protein_id": "ENSP00000424189.2",
          "transcript_support_level": 1,
          "aa_start": 59,
          "aa_end": null,
          "aa_length": 478,
          "cds_start": 176,
          "cds_end": null,
          "cds_length": 1437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000869.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000504030.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.194C>T",
          "hgvs_p": "p.Thr65Ile",
          "transcript": "ENST00000375498.6",
          "protein_id": "ENSP00000364648.2",
          "transcript_support_level": 1,
          "aa_start": 65,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 194,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375498.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.194C>T",
          "hgvs_p": "p.Thr65Ile",
          "transcript": "ENST00000355556.6",
          "protein_id": "ENSP00000347754.2",
          "transcript_support_level": 2,
          "aa_start": 65,
          "aa_end": null,
          "aa_length": 516,
          "cds_start": 194,
          "cds_end": null,
          "cds_length": 1551,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000355556.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.176C>T",
          "hgvs_p": "p.Thr59Ile",
          "transcript": "NM_213621.4",
          "protein_id": "NP_998786.3",
          "transcript_support_level": null,
          "aa_start": 59,
          "aa_end": null,
          "aa_length": 510,
          "cds_start": 176,
          "cds_end": null,
          "cds_length": 1533,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_213621.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.176C>T",
          "hgvs_p": "p.Thr59Ile",
          "transcript": "ENST00000506841.6",
          "protein_id": "ENSP00000424776.2",
          "transcript_support_level": 2,
          "aa_start": 59,
          "aa_end": null,
          "aa_length": 510,
          "cds_start": 176,
          "cds_end": null,
          "cds_length": 1533,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000506841.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.176C>T",
          "hgvs_p": "p.Thr59Ile",
          "transcript": "ENST00000936751.1",
          "protein_id": "ENSP00000606810.1",
          "transcript_support_level": null,
          "aa_start": 59,
          "aa_end": null,
          "aa_length": 477,
          "cds_start": 176,
          "cds_end": null,
          "cds_length": 1434,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936751.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.131C>T",
          "hgvs_p": "p.Thr44Ile",
          "transcript": "NM_001161772.3",
          "protein_id": "NP_001155244.1",
          "transcript_support_level": null,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 131,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001161772.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.131C>T",
          "hgvs_p": "p.Thr44Ile",
          "transcript": "ENST00000299961.5",
          "protein_id": "ENSP00000299961.4",
          "transcript_support_level": 2,
          "aa_start": 44,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 131,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000299961.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "c.176C>T",
          "hgvs_p": "p.Thr59Ile",
          "transcript": "ENST00000936750.1",
          "protein_id": "ENSP00000606809.1",
          "transcript_support_level": null,
          "aa_start": 59,
          "aa_end": null,
          "aa_length": 438,
          "cds_start": 176,
          "cds_end": null,
          "cds_length": 1317,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936750.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "n.176C>T",
          "hgvs_p": null,
          "transcript": "ENST00000510849.5",
          "protein_id": "ENSP00000423653.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000510849.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HTR3A",
          "gene_hgnc_id": 5297,
          "hgvs_c": "n.394C>T",
          "hgvs_p": null,
          "transcript": "NR_046363.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_046363.2"
        }
      ],
      "gene_symbol": "HTR3A",
      "gene_hgnc_id": 5297,
      "dbsnp": "rs770614768",
      "frequency_reference_population": 0.0000018585173,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000136809,
      "gnomad_genomes_af": 0.00000656607,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8544199466705322,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.704,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.3323,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.25,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 5.855,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_213621.4",
          "gene_symbol": "HTR3A",
          "hgnc_id": 5297,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.176C>T",
          "hgvs_p": "p.Thr59Ile"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}