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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 11-22279634-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=11&pos=22279634&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "11",
      "pos": 22279634,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_213599.3",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2611A>C",
          "hgvs_p": "p.Met871Leu",
          "transcript": "NM_213599.3",
          "protein_id": "NP_998764.1",
          "transcript_support_level": null,
          "aa_start": 871,
          "aa_end": null,
          "aa_length": 913,
          "cds_start": 2611,
          "cds_end": null,
          "cds_length": 2742,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000324559.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_213599.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2611A>C",
          "hgvs_p": "p.Met871Leu",
          "transcript": "ENST00000324559.9",
          "protein_id": "ENSP00000315371.9",
          "transcript_support_level": 1,
          "aa_start": 871,
          "aa_end": null,
          "aa_length": 913,
          "cds_start": 2611,
          "cds_end": null,
          "cds_length": 2742,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_213599.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000324559.9"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2608A>C",
          "hgvs_p": "p.Met870Leu",
          "transcript": "NM_001142649.2",
          "protein_id": "NP_001136121.1",
          "transcript_support_level": null,
          "aa_start": 870,
          "aa_end": null,
          "aa_length": 912,
          "cds_start": 2608,
          "cds_end": null,
          "cds_length": 2739,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001142649.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2569A>C",
          "hgvs_p": "p.Met857Leu",
          "transcript": "NM_001410963.1",
          "protein_id": "NP_001397892.1",
          "transcript_support_level": null,
          "aa_start": 857,
          "aa_end": null,
          "aa_length": 899,
          "cds_start": 2569,
          "cds_end": null,
          "cds_length": 2700,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410963.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2569A>C",
          "hgvs_p": "p.Met857Leu",
          "transcript": "ENST00000682341.1",
          "protein_id": "ENSP00000508251.1",
          "transcript_support_level": null,
          "aa_start": 857,
          "aa_end": null,
          "aa_length": 899,
          "cds_start": 2569,
          "cds_end": null,
          "cds_length": 2700,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000682341.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2566A>C",
          "hgvs_p": "p.Met856Leu",
          "transcript": "NM_001410964.1",
          "protein_id": "NP_001397893.1",
          "transcript_support_level": null,
          "aa_start": 856,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": 2566,
          "cds_end": null,
          "cds_length": 2697,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410964.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2566A>C",
          "hgvs_p": "p.Met856Leu",
          "transcript": "ENST00000684663.1",
          "protein_id": "ENSP00000508009.1",
          "transcript_support_level": null,
          "aa_start": 856,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": 2566,
          "cds_end": null,
          "cds_length": 2697,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000684663.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2533A>C",
          "hgvs_p": "p.Met845Leu",
          "transcript": "NM_001441294.1",
          "protein_id": "NP_001428223.1",
          "transcript_support_level": null,
          "aa_start": 845,
          "aa_end": null,
          "aa_length": 887,
          "cds_start": 2533,
          "cds_end": null,
          "cds_length": 2664,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441294.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2530A>C",
          "hgvs_p": "p.Met844Leu",
          "transcript": "NM_001441295.1",
          "protein_id": "NP_001428224.1",
          "transcript_support_level": null,
          "aa_start": 844,
          "aa_end": null,
          "aa_length": 886,
          "cds_start": 2530,
          "cds_end": null,
          "cds_length": 2661,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441295.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2518A>C",
          "hgvs_p": "p.Met840Leu",
          "transcript": "NM_001441296.1",
          "protein_id": "NP_001428225.1",
          "transcript_support_level": null,
          "aa_start": 840,
          "aa_end": null,
          "aa_length": 882,
          "cds_start": 2518,
          "cds_end": null,
          "cds_length": 2649,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441296.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2497A>C",
          "hgvs_p": "p.Met833Leu",
          "transcript": "ENST00000950081.1",
          "protein_id": "ENSP00000620140.1",
          "transcript_support_level": null,
          "aa_start": 833,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 2497,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950081.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2491A>C",
          "hgvs_p": "p.Met831Leu",
          "transcript": "NM_001441297.1",
          "protein_id": "NP_001428226.1",
          "transcript_support_level": null,
          "aa_start": 831,
          "aa_end": null,
          "aa_length": 873,
          "cds_start": 2491,
          "cds_end": null,
          "cds_length": 2622,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441297.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2455A>C",
          "hgvs_p": "p.Met819Leu",
          "transcript": "NM_001441298.1",
          "protein_id": "NP_001428227.1",
          "transcript_support_level": null,
          "aa_start": 819,
          "aa_end": null,
          "aa_length": 861,
          "cds_start": 2455,
          "cds_end": null,
          "cds_length": 2586,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441298.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2455A>C",
          "hgvs_p": "p.Met819Leu",
          "transcript": "ENST00000878905.1",
          "protein_id": "ENSP00000548964.1",
          "transcript_support_level": null,
          "aa_start": 819,
          "aa_end": null,
          "aa_length": 861,
          "cds_start": 2455,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000878905.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2452A>C",
          "hgvs_p": "p.Met818Leu",
          "transcript": "NM_001441299.1",
          "protein_id": "NP_001428228.1",
          "transcript_support_level": null,
          "aa_start": 818,
          "aa_end": null,
          "aa_length": 860,
          "cds_start": 2452,
          "cds_end": null,
          "cds_length": 2583,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441299.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2452A>C",
          "hgvs_p": "p.Met818Leu",
          "transcript": "ENST00000878904.1",
          "protein_id": "ENSP00000548963.1",
          "transcript_support_level": null,
          "aa_start": 818,
          "aa_end": null,
          "aa_length": 860,
          "cds_start": 2452,
          "cds_end": null,
          "cds_length": 2583,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878904.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2254A>C",
          "hgvs_p": "p.Met752Leu",
          "transcript": "ENST00000950082.1",
          "protein_id": "ENSP00000620141.1",
          "transcript_support_level": null,
          "aa_start": 752,
          "aa_end": null,
          "aa_length": 794,
          "cds_start": 2254,
          "cds_end": null,
          "cds_length": 2385,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000950082.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2176A>C",
          "hgvs_p": "p.Met726Leu",
          "transcript": "NM_001441300.1",
          "protein_id": "NP_001428229.1",
          "transcript_support_level": null,
          "aa_start": 726,
          "aa_end": null,
          "aa_length": 768,
          "cds_start": 2176,
          "cds_end": null,
          "cds_length": 2307,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001441300.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2176A>C",
          "hgvs_p": "p.Met726Leu",
          "transcript": "ENST00000939029.1",
          "protein_id": "ENSP00000609088.1",
          "transcript_support_level": null,
          "aa_start": 726,
          "aa_end": null,
          "aa_length": 768,
          "cds_start": 2176,
          "cds_end": null,
          "cds_length": 2307,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939029.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.2173A>C",
          "hgvs_p": "p.Met725Leu",
          "transcript": "NM_001441301.1",
          "protein_id": "NP_001428230.1",
          "transcript_support_level": null,
          "aa_start": 725,
          "aa_end": null,
          "aa_length": 767,
          "cds_start": 2173,
          "cds_end": null,
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      "computational_score_selected": 0.02430519461631775,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
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      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.5,
      "bayesdelnoaf_prediction": "Benign",
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      "phylop100way_prediction": "Benign",
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      "acmg_classification": "Likely_benign",
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      "acmg_by_gene": [
        {
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          "transcript": "NM_213599.3",
          "gene_symbol": "ANO5",
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          "hgvs_p": "p.Met871Leu"
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      ],
      "clinvar_disease": "Autosomal recessive limb-girdle muscular dystrophy type 2L,Gnathodiaphyseal dysplasia,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Autosomal recessive limb-girdle muscular dystrophy type 2L;Gnathodiaphyseal dysplasia|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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