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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 11-418559-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=11&pos=418559&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "11",
      "pos": 418559,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001012302.3",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2161G>A",
          "hgvs_p": "p.Ala721Thr",
          "transcript": "NM_001012302.3",
          "protein_id": "NP_001012302.2",
          "transcript_support_level": null,
          "aa_start": 721,
          "aa_end": null,
          "aa_length": 782,
          "cds_start": 2161,
          "cds_end": null,
          "cds_length": 2349,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000332826.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001012302.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2161G>A",
          "hgvs_p": "p.Ala721Thr",
          "transcript": "ENST00000332826.7",
          "protein_id": "ENSP00000332788.6",
          "transcript_support_level": 1,
          "aa_start": 721,
          "aa_end": null,
          "aa_length": 782,
          "cds_start": 2161,
          "cds_end": null,
          "cds_length": 2349,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001012302.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000332826.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "n.653G>A",
          "hgvs_p": null,
          "transcript": "ENST00000524802.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000524802.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "n.2272G>A",
          "hgvs_p": null,
          "transcript": "ENST00000528927.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000528927.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2302G>A",
          "hgvs_p": "p.Ala768Thr",
          "transcript": "ENST00000884108.1",
          "protein_id": "ENSP00000554167.1",
          "transcript_support_level": null,
          "aa_start": 768,
          "aa_end": null,
          "aa_length": 829,
          "cds_start": 2302,
          "cds_end": null,
          "cds_length": 2490,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884108.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2197G>A",
          "hgvs_p": "p.Ala733Thr",
          "transcript": "ENST00000884106.1",
          "protein_id": "ENSP00000554165.1",
          "transcript_support_level": null,
          "aa_start": 733,
          "aa_end": null,
          "aa_length": 794,
          "cds_start": 2197,
          "cds_end": null,
          "cds_length": 2385,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884106.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2191G>A",
          "hgvs_p": "p.Ala731Thr",
          "transcript": "ENST00000884103.1",
          "protein_id": "ENSP00000554162.1",
          "transcript_support_level": null,
          "aa_start": 731,
          "aa_end": null,
          "aa_length": 792,
          "cds_start": 2191,
          "cds_end": null,
          "cds_length": 2379,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884103.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2185G>A",
          "hgvs_p": "p.Ala729Thr",
          "transcript": "ENST00000884111.1",
          "protein_id": "ENSP00000554170.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 790,
          "cds_start": 2185,
          "cds_end": null,
          "cds_length": 2373,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884111.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2155G>A",
          "hgvs_p": "p.Ala719Thr",
          "transcript": "ENST00000884101.1",
          "protein_id": "ENSP00000554160.1",
          "transcript_support_level": null,
          "aa_start": 719,
          "aa_end": null,
          "aa_length": 780,
          "cds_start": 2155,
          "cds_end": null,
          "cds_length": 2343,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884101.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2155G>A",
          "hgvs_p": "p.Ala719Thr",
          "transcript": "ENST00000884104.1",
          "protein_id": "ENSP00000554163.1",
          "transcript_support_level": null,
          "aa_start": 719,
          "aa_end": null,
          "aa_length": 780,
          "cds_start": 2155,
          "cds_end": null,
          "cds_length": 2343,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884104.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2095G>A",
          "hgvs_p": "p.Ala699Thr",
          "transcript": "ENST00000884110.1",
          "protein_id": "ENSP00000554169.1",
          "transcript_support_level": null,
          "aa_start": 699,
          "aa_end": null,
          "aa_length": 760,
          "cds_start": 2095,
          "cds_end": null,
          "cds_length": 2283,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884110.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2086G>A",
          "hgvs_p": "p.Ala696Thr",
          "transcript": "ENST00000884105.1",
          "protein_id": "ENSP00000554164.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 757,
          "cds_start": 2086,
          "cds_end": null,
          "cds_length": 2274,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884105.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2080G>A",
          "hgvs_p": "p.Ala694Thr",
          "transcript": "ENST00000924670.1",
          "protein_id": "ENSP00000594729.1",
          "transcript_support_level": null,
          "aa_start": 694,
          "aa_end": null,
          "aa_length": 755,
          "cds_start": 2080,
          "cds_end": null,
          "cds_length": 2268,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000924670.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2032G>A",
          "hgvs_p": "p.Ala678Thr",
          "transcript": "ENST00000884109.1",
          "protein_id": "ENSP00000554168.1",
          "transcript_support_level": null,
          "aa_start": 678,
          "aa_end": null,
          "aa_length": 739,
          "cds_start": 2032,
          "cds_end": null,
          "cds_length": 2220,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884109.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2008G>A",
          "hgvs_p": "p.Ala670Thr",
          "transcript": "ENST00000884107.1",
          "protein_id": "ENSP00000554166.1",
          "transcript_support_level": null,
          "aa_start": 670,
          "aa_end": null,
          "aa_length": 731,
          "cds_start": 2008,
          "cds_end": null,
          "cds_length": 2196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884107.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.2002G>A",
          "hgvs_p": "p.Ala668Thr",
          "transcript": "ENST00000884102.1",
          "protein_id": "ENSP00000554161.1",
          "transcript_support_level": null,
          "aa_start": 668,
          "aa_end": null,
          "aa_length": 729,
          "cds_start": 2002,
          "cds_end": null,
          "cds_length": 2190,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884102.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "c.1729G>A",
          "hgvs_p": "p.Ala577Thr",
          "transcript": "NM_001347882.2",
          "protein_id": "NP_001334811.1",
          "transcript_support_level": null,
          "aa_start": 577,
          "aa_end": null,
          "aa_length": 638,
          "cds_start": 1729,
          "cds_end": null,
          "cds_length": 1917,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001347882.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "n.2242G>A",
          "hgvs_p": null,
          "transcript": "ENST00000526142.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000526142.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "n.4866G>A",
          "hgvs_p": null,
          "transcript": "ENST00000532094.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000532094.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO9",
          "gene_hgnc_id": 20679,
          "hgvs_c": "n.1534G>A",
          "hgvs_p": null,
          "transcript": "ENST00000534161.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000534161.5"
        }
      ],
      "gene_symbol": "ANO9",
      "gene_hgnc_id": 20679,
      "dbsnp": "rs753401969",
      "frequency_reference_population": 0.00001798008,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 29,
      "gnomad_exomes_af": 0.0000184845,
      "gnomad_genomes_af": 0.0000131397,
      "gnomad_exomes_ac": 27,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7975773811340332,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.451,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.3738,
      "alphamissense_prediction": "Uncertain_significance",
      "bayesdelnoaf_score": -0.12,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.289,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001012302.3",
          "gene_symbol": "ANO9",
          "hgnc_id": 20679,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2161G>A",
          "hgvs_p": "p.Ala721Thr"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}