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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 11-74949325-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=11&pos=74949325&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "11",
      "pos": 74949325,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_014752.3",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "NM_014752.3",
          "protein_id": "NP_055567.2",
          "transcript_support_level": null,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 226,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 681,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000263672.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014752.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000263672.11",
          "protein_id": "ENSP00000263672.6",
          "transcript_support_level": 1,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 226,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 681,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_014752.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000263672.11"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000532972.5",
          "protein_id": "ENSP00000436759.1",
          "transcript_support_level": 2,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 257,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 774,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000532972.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000931774.1",
          "protein_id": "ENSP00000601833.1",
          "transcript_support_level": null,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 257,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 774,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931774.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000931772.1",
          "protein_id": "ENSP00000601831.1",
          "transcript_support_level": null,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 224,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 675,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931772.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000931776.1",
          "protein_id": "ENSP00000601835.1",
          "transcript_support_level": null,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931776.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000931775.1",
          "protein_id": "ENSP00000601834.1",
          "transcript_support_level": null,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 203,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 612,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931775.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000530257.5",
          "protein_id": "ENSP00000433439.1",
          "transcript_support_level": 3,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 157,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 474,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000530257.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000931771.1",
          "protein_id": "ENSP00000601830.1",
          "transcript_support_level": null,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931771.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly",
          "transcript": "ENST00000931773.1",
          "protein_id": "ENSP00000601832.1",
          "transcript_support_level": null,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 129,
          "cds_start": 40,
          "cds_end": null,
          "cds_length": 390,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000931773.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "c.-142A>G",
          "hgvs_p": null,
          "transcript": "ENST00000526361.1",
          "protein_id": "ENSP00000434248.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 87,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 264,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000526361.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "n.47A>G",
          "hgvs_p": null,
          "transcript": "ENST00000527225.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000527225.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "n.55A>G",
          "hgvs_p": null,
          "transcript": "ENST00000527290.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000527290.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPCS2",
          "gene_hgnc_id": 28962,
          "hgvs_c": "n.48A>G",
          "hgvs_p": null,
          "transcript": "ENST00000528265.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000528265.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "XRRA1",
          "gene_hgnc_id": 18868,
          "hgvs_c": "c.-470T>C",
          "hgvs_p": null,
          "transcript": "NM_001378157.1",
          "protein_id": "NP_001365086.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 800,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2403,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000684022.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001378157.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "XRRA1",
          "gene_hgnc_id": 18868,
          "hgvs_c": "c.-470T>C",
          "hgvs_p": null,
          "transcript": "ENST00000684022.1",
          "protein_id": "ENSP00000507107.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 800,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2403,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001378157.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000684022.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "XRRA1",
          "gene_hgnc_id": 18868,
          "hgvs_c": "c.-470T>C",
          "hgvs_p": null,
          "transcript": "ENST00000527087.5",
          "protein_id": "ENSP00000435838.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 598,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1797,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000527087.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "XRRA1",
          "gene_hgnc_id": 18868,
          "hgvs_c": "c.-1027T>C",
          "hgvs_p": null,
          "transcript": "ENST00000321448.12",
          "protein_id": "ENSP00000319303.8",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 517,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1554,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000321448.12"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "XRRA1",
          "gene_hgnc_id": 18868,
          "hgvs_c": "c.-293T>C",
          "hgvs_p": null,
          "transcript": "ENST00000705661.1",
          "protein_id": "ENSP00000516156.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2556,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000705661.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "XRRA1",
          "gene_hgnc_id": 18868,
          "hgvs_c": "c.-470T>C",
          "hgvs_p": null,
          "transcript": "NM_182969.4",
          "protein_id": "NP_892014.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 792,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2379,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_182969.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
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        {
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      ],
      "gene_symbol": "SPCS2",
      "gene_hgnc_id": 28962,
      "dbsnp": "rs1054747868",
      "frequency_reference_population": 0.000012254049,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 19,
      "gnomad_exomes_af": 0.0000121575,
      "gnomad_genomes_af": 0.0000131413,
      "gnomad_exomes_ac": 17,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.02051493525505066,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.043,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0492,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.47,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.675,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -10,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -10,
          "benign_score": 10,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Moderate",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_014752.3",
          "gene_symbol": "SPCS2",
          "hgnc_id": 28962,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.40A>G",
          "hgvs_p": "p.Ser14Gly"
        },
        {
          "score": -4,
          "benign_score": 6,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001378157.1",
          "gene_symbol": "XRRA1",
          "hgnc_id": 18868,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-470T>C",
          "hgvs_p": null
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}