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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 11-87309668-A-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=11&pos=87309668&ref=A&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "11",
      "pos": 87309668,
      "ref": "A",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_022918.4",
      "consequences": [
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.932A>T",
          "hgvs_p": "p.Tyr311Phe",
          "transcript": "NM_022918.4",
          "protein_id": "NP_075069.3",
          "transcript_support_level": null,
          "aa_start": 311,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 932,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000305494.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_022918.4"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.932A>T",
          "hgvs_p": "p.Tyr311Phe",
          "transcript": "ENST00000305494.6",
          "protein_id": "ENSP00000306344.5",
          "transcript_support_level": 1,
          "aa_start": 311,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 932,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_022918.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000305494.6"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.866A>T",
          "hgvs_p": "p.Tyr289Phe",
          "transcript": "ENST00000340353.11",
          "protein_id": "ENSP00000345513.6",
          "transcript_support_level": 1,
          "aa_start": 289,
          "aa_end": null,
          "aa_length": 436,
          "cds_start": 866,
          "cds_end": null,
          "cds_length": 1311,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000340353.11"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.977A>T",
          "hgvs_p": "p.Tyr326Phe",
          "transcript": "ENST00000954970.1",
          "protein_id": "ENSP00000625029.1",
          "transcript_support_level": null,
          "aa_start": 326,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 977,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000954970.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.929A>T",
          "hgvs_p": "p.Tyr310Phe",
          "transcript": "ENST00000934696.1",
          "protein_id": "ENSP00000604755.1",
          "transcript_support_level": null,
          "aa_start": 310,
          "aa_end": null,
          "aa_length": 457,
          "cds_start": 929,
          "cds_end": null,
          "cds_length": 1374,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000934696.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.890A>T",
          "hgvs_p": "p.Tyr297Phe",
          "transcript": "ENST00000854944.1",
          "protein_id": "ENSP00000525003.1",
          "transcript_support_level": null,
          "aa_start": 297,
          "aa_end": null,
          "aa_length": 444,
          "cds_start": 890,
          "cds_end": null,
          "cds_length": 1335,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854944.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.866A>T",
          "hgvs_p": "p.Tyr289Phe",
          "transcript": "NM_001168724.2",
          "protein_id": "NP_001162195.1",
          "transcript_support_level": null,
          "aa_start": 289,
          "aa_end": null,
          "aa_length": 436,
          "cds_start": 866,
          "cds_end": null,
          "cds_length": 1311,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001168724.2"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.839A>T",
          "hgvs_p": "p.Tyr280Phe",
          "transcript": "ENST00000854940.1",
          "protein_id": "ENSP00000524999.1",
          "transcript_support_level": null,
          "aa_start": 280,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 839,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854940.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.932A>T",
          "hgvs_p": "p.Tyr311Phe",
          "transcript": "ENST00000854942.1",
          "protein_id": "ENSP00000525001.1",
          "transcript_support_level": null,
          "aa_start": 311,
          "aa_end": null,
          "aa_length": 411,
          "cds_start": 932,
          "cds_end": null,
          "cds_length": 1236,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854942.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.785A>T",
          "hgvs_p": "p.Tyr262Phe",
          "transcript": "ENST00000954972.1",
          "protein_id": "ENSP00000625031.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 409,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 1230,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000954972.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.782A>T",
          "hgvs_p": "p.Tyr261Phe",
          "transcript": "ENST00000954969.1",
          "protein_id": "ENSP00000625028.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 408,
          "cds_start": 782,
          "cds_end": null,
          "cds_length": 1227,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000954969.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.773A>T",
          "hgvs_p": "p.Tyr258Phe",
          "transcript": "ENST00000854945.1",
          "protein_id": "ENSP00000525004.1",
          "transcript_support_level": null,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 405,
          "cds_start": 773,
          "cds_end": null,
          "cds_length": 1218,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854945.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.743A>T",
          "hgvs_p": "p.Tyr248Phe",
          "transcript": "ENST00000854946.1",
          "protein_id": "ENSP00000525005.1",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854946.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.866A>T",
          "hgvs_p": "p.Tyr289Phe",
          "transcript": "ENST00000954971.1",
          "protein_id": "ENSP00000625030.1",
          "transcript_support_level": null,
          "aa_start": 289,
          "aa_end": null,
          "aa_length": 389,
          "cds_start": 866,
          "cds_end": null,
          "cds_length": 1170,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.677A>T",
          "hgvs_p": "p.Tyr226Phe",
          "transcript": "ENST00000854941.1",
          "protein_id": "ENSP00000525000.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 677,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854941.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.611A>T",
          "hgvs_p": "p.Tyr204Phe",
          "transcript": "ENST00000934697.1",
          "protein_id": "ENSP00000604756.1",
          "transcript_support_level": null,
          "aa_start": 204,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 611,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000934697.1"
        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.545A>T",
          "hgvs_p": "p.Tyr182Phe",
          "transcript": "ENST00000532959.5",
          "protein_id": "ENSP00000436179.1",
          "transcript_support_level": 2,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 329,
          "cds_start": 545,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "Y",
          "aa_alt": "F",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.548A>T",
          "hgvs_p": "p.Tyr183Phe",
          "transcript": "XM_017018141.3",
          "protein_id": "XP_016873630.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 330,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 993,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017018141.3"
        },
        {
          "aa_ref": "Y",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.515A>T",
          "hgvs_p": "p.Tyr172Phe",
          "transcript": "XM_017018142.2",
          "protein_id": "XP_016873631.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 319,
          "cds_start": 515,
          "cds_end": null,
          "cds_length": 960,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017018142.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "c.768+3663A>T",
          "hgvs_p": null,
          "transcript": "ENST00000854943.1",
          "protein_id": "ENSP00000525002.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 402,
          "cds_start": null,
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          "cds_length": 1209,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000854943.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TMEM135",
          "gene_hgnc_id": 26167,
          "hgvs_c": "n.1010A>T",
          "hgvs_p": null,
          "transcript": "NR_033149.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_033149.2"
        }
      ],
      "gene_symbol": "TMEM135",
      "gene_hgnc_id": 26167,
      "dbsnp": "rs776270617",
      "frequency_reference_population": 0.00000342229,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000342229,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 5,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5263512134552002,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.2,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2709,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.25,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 9.15,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_022918.4",
          "gene_symbol": "TMEM135",
          "hgnc_id": 26167,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.932A>T",
          "hgvs_p": "p.Tyr311Phe"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}