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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-109933156-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=109933156&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 109933156,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_057169.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2102G>A",
          "hgvs_p": "p.Arg701His",
          "transcript": "NM_057169.5",
          "protein_id": "NP_476510.1",
          "transcript_support_level": null,
          "aa_start": 701,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 2102,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000355312.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_057169.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2102G>A",
          "hgvs_p": "p.Arg701His",
          "transcript": "ENST00000355312.8",
          "protein_id": "ENSP00000347464.3",
          "transcript_support_level": 1,
          "aa_start": 701,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 2102,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_057169.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000355312.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1868G>A",
          "hgvs_p": "p.Arg623His",
          "transcript": "ENST00000457474.6",
          "protein_id": "ENSP00000391813.2",
          "transcript_support_level": 1,
          "aa_start": 623,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": 1868,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000457474.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2099G>A",
          "hgvs_p": "p.Arg700His",
          "transcript": "ENST00000876497.1",
          "protein_id": "ENSP00000546556.1",
          "transcript_support_level": null,
          "aa_start": 700,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 2099,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876497.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2099G>A",
          "hgvs_p": "p.Arg700His",
          "transcript": "ENST00000876510.1",
          "protein_id": "ENSP00000546569.1",
          "transcript_support_level": null,
          "aa_start": 700,
          "aa_end": null,
          "aa_length": 758,
          "cds_start": 2099,
          "cds_end": null,
          "cds_length": 2277,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876510.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2057G>A",
          "hgvs_p": "p.Arg686His",
          "transcript": "ENST00000876499.1",
          "protein_id": "ENSP00000546558.1",
          "transcript_support_level": null,
          "aa_start": 686,
          "aa_end": null,
          "aa_length": 744,
          "cds_start": 2057,
          "cds_end": null,
          "cds_length": 2235,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876499.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2054G>A",
          "hgvs_p": "p.Arg685His",
          "transcript": "ENST00000876494.1",
          "protein_id": "ENSP00000546553.1",
          "transcript_support_level": null,
          "aa_start": 685,
          "aa_end": null,
          "aa_length": 743,
          "cds_start": 2054,
          "cds_end": null,
          "cds_length": 2232,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876494.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2012G>A",
          "hgvs_p": "p.Arg671His",
          "transcript": "NM_001135214.3",
          "protein_id": "NP_001128686.1",
          "transcript_support_level": null,
          "aa_start": 671,
          "aa_end": null,
          "aa_length": 729,
          "cds_start": 2012,
          "cds_end": null,
          "cds_length": 2190,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001135214.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2012G>A",
          "hgvs_p": "p.Arg671His",
          "transcript": "ENST00000361006.9",
          "protein_id": "ENSP00000354282.5",
          "transcript_support_level": 5,
          "aa_start": 671,
          "aa_end": null,
          "aa_length": 729,
          "cds_start": 2012,
          "cds_end": null,
          "cds_length": 2190,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361006.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.2009G>A",
          "hgvs_p": "p.Arg670His",
          "transcript": "ENST00000876504.1",
          "protein_id": "ENSP00000546563.1",
          "transcript_support_level": null,
          "aa_start": 670,
          "aa_end": null,
          "aa_length": 728,
          "cds_start": 2009,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1967G>A",
          "hgvs_p": "p.Arg656His",
          "transcript": "ENST00000876503.1",
          "protein_id": "ENSP00000546562.1",
          "transcript_support_level": null,
          "aa_start": 656,
          "aa_end": null,
          "aa_length": 714,
          "cds_start": 1967,
          "cds_end": null,
          "cds_length": 2145,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876503.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1964G>A",
          "hgvs_p": "p.Arg655His",
          "transcript": "ENST00000935696.1",
          "protein_id": "ENSP00000605755.1",
          "transcript_support_level": null,
          "aa_start": 655,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 1964,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000935696.1"
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        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1952G>A",
          "hgvs_p": "p.Arg651His",
          "transcript": "ENST00000876495.1",
          "protein_id": "ENSP00000546554.1",
          "transcript_support_level": null,
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          "aa_length": 709,
          "cds_start": 1952,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000876495.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1949G>A",
          "hgvs_p": "p.Arg650His",
          "transcript": "NM_001330153.2",
          "protein_id": "NP_001317082.1",
          "transcript_support_level": null,
          "aa_start": 650,
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          "cds_start": 1949,
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          "cdna_start": null,
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        {
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          "intron_rank": null,
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          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1949G>A",
          "hgvs_p": "p.Arg650His",
          "transcript": "ENST00000551209.5",
          "protein_id": "ENSP00000448832.1",
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          "aa_length": 708,
          "cds_start": 1949,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000551209.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1868G>A",
          "hgvs_p": "p.Arg623His",
          "transcript": "NM_001135213.3",
          "protein_id": "NP_001128685.1",
          "transcript_support_level": null,
          "aa_start": 623,
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          "aa_length": 681,
          "cds_start": 1868,
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          "biotype": "protein_coding",
          "feature": "NM_001135213.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1862G>A",
          "hgvs_p": "p.Arg621His",
          "transcript": "NM_014776.5",
          "protein_id": "NP_055591.2",
          "transcript_support_level": null,
          "aa_start": 621,
          "aa_end": null,
          "aa_length": 679,
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          "biotype": "protein_coding",
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        },
        {
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          "exon_count": 18,
          "intron_rank": null,
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          "gene_symbol": "GIT2",
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          "hgvs_c": "c.1862G>A",
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          "transcript": "ENST00000876500.1",
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          "cds_start": 1862,
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          "biotype": "protein_coding",
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        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "GIT2",
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          "hgvs_c": "c.1859G>A",
          "hgvs_p": "p.Arg620His",
          "transcript": "ENST00000876509.1",
          "protein_id": "ENSP00000546568.1",
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          "aa_start": 620,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000876509.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GIT2",
          "gene_hgnc_id": 4273,
          "hgvs_c": "c.1853G>A",
          "hgvs_p": "p.Arg618His",
          "transcript": "ENST00000876501.1",
          "protein_id": "ENSP00000546560.1",
          "transcript_support_level": null,
          "aa_start": 618,
          "aa_end": null,
          "aa_length": 676,
          "cds_start": 1853,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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        {
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          "biotype": "nonsense_mediated_decay",
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        {
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          "biotype": "pseudogene",
          "feature": "ENST00000550780.1"
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      ],
      "gene_symbol": "GIT2",
      "gene_hgnc_id": 4273,
      "dbsnp": "rs753666949",
      "frequency_reference_population": 0.0000468057,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 74,
      "gnomad_exomes_af": 0.0000475912,
      "gnomad_genomes_af": 0.0000394301,
      "gnomad_exomes_ac": 68,
      "gnomad_genomes_ac": 6,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.37204933166503906,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.506,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1133,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.22,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.905,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_057169.5",
          "gene_symbol": "GIT2",
          "hgnc_id": 4273,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.2102G>A",
          "hgvs_p": "p.Arg701His"
        },
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000549524.1",
          "gene_symbol": "TCHP",
          "hgnc_id": 28135,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "n.2433C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}