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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-123707685-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=123707685&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 123707685,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000303372.7",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.2066G>C",
          "hgvs_p": "p.Trp689Ser",
          "transcript": "NM_024809.5",
          "protein_id": "NP_079085.2",
          "transcript_support_level": null,
          "aa_start": 689,
          "aa_end": null,
          "aa_length": 697,
          "cds_start": 2066,
          "cds_end": null,
          "cds_length": 2094,
          "cdna_start": 2194,
          "cdna_end": null,
          "cdna_length": 2908,
          "mane_select": "ENST00000303372.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.2066G>C",
          "hgvs_p": "p.Trp689Ser",
          "transcript": "ENST00000303372.7",
          "protein_id": "ENSP00000304941.5",
          "transcript_support_level": 1,
          "aa_start": 689,
          "aa_end": null,
          "aa_length": 697,
          "cds_start": 2066,
          "cds_end": null,
          "cds_length": 2094,
          "cdna_start": 2194,
          "cdna_end": null,
          "cdna_length": 2908,
          "mane_select": "NM_024809.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.2063G>C",
          "hgvs_p": "p.Trp688Ser",
          "transcript": "NM_001143850.3",
          "protein_id": "NP_001137322.1",
          "transcript_support_level": null,
          "aa_start": 688,
          "aa_end": null,
          "aa_length": 696,
          "cds_start": 2063,
          "cds_end": null,
          "cds_length": 2091,
          "cdna_start": 2191,
          "cdna_end": null,
          "cdna_length": 2905,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.2063G>C",
          "hgvs_p": "p.Trp688Ser",
          "transcript": "ENST00000426174.6",
          "protein_id": "ENSP00000395171.2",
          "transcript_support_level": 2,
          "aa_start": 688,
          "aa_end": null,
          "aa_length": 696,
          "cds_start": 2063,
          "cds_end": null,
          "cds_length": 2091,
          "cdna_start": 2191,
          "cdna_end": null,
          "cdna_length": 2513,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.1931G>C",
          "hgvs_p": "p.Trp644Ser",
          "transcript": "NM_001410989.1",
          "protein_id": "NP_001397918.1",
          "transcript_support_level": null,
          "aa_start": 644,
          "aa_end": null,
          "aa_length": 652,
          "cds_start": 1931,
          "cds_end": null,
          "cds_length": 1959,
          "cdna_start": 2059,
          "cdna_end": null,
          "cdna_length": 2773,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.1931G>C",
          "hgvs_p": "p.Trp644Ser",
          "transcript": "ENST00000680574.1",
          "protein_id": "ENSP00000505356.1",
          "transcript_support_level": null,
          "aa_start": 644,
          "aa_end": null,
          "aa_length": 652,
          "cds_start": 1931,
          "cds_end": null,
          "cds_length": 1959,
          "cdna_start": 2062,
          "cdna_end": null,
          "cdna_length": 2374,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.1928G>C",
          "hgvs_p": "p.Trp643Ser",
          "transcript": "XM_017019974.2",
          "protein_id": "XP_016875463.1",
          "transcript_support_level": null,
          "aa_start": 643,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 1928,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": 2056,
          "cdna_end": null,
          "cdna_length": 2770,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "W",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.1154G>C",
          "hgvs_p": "p.Trp385Ser",
          "transcript": "XM_047429553.1",
          "protein_id": "XP_047285509.1",
          "transcript_support_level": null,
          "aa_start": 385,
          "aa_end": null,
          "aa_length": 393,
          "cds_start": 1154,
          "cds_end": null,
          "cds_length": 1182,
          "cdna_start": 1266,
          "cdna_end": null,
          "cdna_length": 1980,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000255839",
          "gene_hgnc_id": null,
          "hgvs_c": "n.238C>G",
          "hgvs_p": null,
          "transcript": "ENST00000538837.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 321,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "n.1167G>C",
          "hgvs_p": null,
          "transcript": "ENST00000680394.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1496,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "n.*438G>C",
          "hgvs_p": null,
          "transcript": "ENST00000680500.1",
          "protein_id": "ENSP00000506438.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2497,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "n.*438G>C",
          "hgvs_p": null,
          "transcript": "ENST00000680500.1",
          "protein_id": "ENSP00000506438.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2497,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": 17,
          "intron_rank_end": null,
          "gene_symbol": "TCTN2",
          "gene_hgnc_id": 25774,
          "hgvs_c": "c.1982-519G>C",
          "hgvs_p": null,
          "transcript": "ENST00000679504.1",
          "protein_id": "ENSP00000505006.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 669,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2010,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2246,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "TCTN2",
      "gene_hgnc_id": 25774,
      "dbsnp": "rs771335558",
      "frequency_reference_population": 0.000006815323,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 11,
      "gnomad_exomes_af": 0.00000410432,
      "gnomad_genomes_af": 0.0000328653,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7377206087112427,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.649,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.1775,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.09,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 6.686,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000303372.7",
          "gene_symbol": "TCTN2",
          "hgnc_id": 25774,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2066G>C",
          "hgvs_p": "p.Trp689Ser"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000538837.1",
          "gene_symbol": "ENSG00000255839",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.238C>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " type 8,Inborn genetic diseases,Joubert syndrome,Joubert syndrome 24,Meckel syndrome,Meckel-Gruber syndrome",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:3",
      "phenotype_combined": "Meckel-Gruber syndrome;Joubert syndrome|Meckel syndrome, type 8;Joubert syndrome 24|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}